Estrogenic activity of tropical fish food can alter baseline vitellogenin concentrations in male fathead minnow (<i>Pimephales promelas)</i>

Beresford, Nicola; Brian, Jayne V.; Runnalls, Tamsin J.; Sumpter, John P.; Jobling, Susan

doi:10.1002/etc.479

Cited by 23 publications

(12 citation statements)

References 41 publications

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“…In xenoestrogen-contaminated waters, male M. galloprovincialis , displayed elevated VTG while females did not (Pampanin et al, 2005) yet in D. polymorpha females showed a 40% higher rise in VTG than males (Quinn et al, 2004), and Gagné et al (2001) reported increased VTG in both sexes of E. complanata . In addition, recent reports of estrogenic activity of commercial fish food (Beresford et al, 2011) suggest that feeding regimens may contribute to increases in VTG seen in lab settings; yet the low VTG values in control males in our study indicate a minimal effect from feeding. Thus, the short exposure of 6 hr and/or the 100 μg/L estradiol used here may not have been appropriate for inducing VTG in Elliptio complanata .…”

Section: Discussioncontrasting

confidence: 61%

Burrowing in the Freshwater MusselElliptio complanatais Sexually Dimorphic and Feminized by Low Levels of Atrazine

Flynn

Wedin

Bonventre

et al. 2013

Journal of Toxicology and Environmental Health, Part A

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The widely used herbicide atrazine (ATR) may have endocrine-associated adverse effects, including on behavior. In this study, 120 adult freshwater mussels, Elliptio complanata, were exposed to ATR at the environmentally-relevant concentrations of 1.5, 15, or 150 μg/L. Burrowing depth was evaluated hourly for 6 hr and at sacrifice animals were sexed by gonad smear. Female controls burrowed overall approximately 30% less than males, the first report of sexual dimorphism in this behavior. Atrazine at 15 μg/L feminized burrowing in both sexes in that exposed animals burrowed 20% less than their same sex controls. Males treated with 1.5 μg /L ATR displayed approximately 20-fold higher vitellogenin (VTG) levels than same sex controls. Higher concentrations of ATR were not associated with increasing effects. A scatterplot showed a weak binomial curve associating low burrowing with high VTG levels. Taken together, these data suggest a non-linear dose-response in behavioral and physiological feminization produced by ATR and support the need to reconsider the widespread use of this compound.

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Section: Discussioncontrasting

confidence: 61%

Burrowing in the Freshwater MusselElliptio complanatais Sexually Dimorphic and Feminized by Low Levels of Atrazine

Flynn

Wedin

Bonventre

et al. 2013

Journal of Toxicology and Environmental Health, Part A

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“…The estrogenicity caused by commercial fish feeds has been reported in previous studies (Miyahara 403 et al, 2003;Matsumoto et al, 2004;Beresford et al, 2011). In the present work, 11 out of 32 diets 404 induced sbER-mediated transcriptional activity.…”

Section: Estrogenic Activity Of Sewage Water 274supporting

confidence: 84%

“…daidzein and genistein, in the bile of these fish (Kausik et al, 1995). In fact, 67 estrogenicity of commercial fish feeds has already been assessed using yeast estrogen-screen assays 68 (Matsumoto et al, 2004) or in vivo experiments (Beresford et al, 2011). 69…”

Section: Quesada Et Al Highlightsmentioning

confidence: 99%

Assessment of estrogenic and thyrogenic activities in fish feeds

et al. 2012

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Normal functioning of the endocrine system is essential for the proper development and reproduction of animals. Substances interfering with its homeostasis are called endocrine disruptors (EDs) and may represent a risk for the health of the organism. One of the mechanisms of endocrine disruption that has attracted great attention in recent years concerns alterations in the normal functioning of the estrogen receptor (ER), but far less attention has been paid to those substances interfering with the thyroid axis, which, in fish, plays several critical roles in a variety of biological functions. In aquaculture, feedstuffs can be a source of hormones or persistent pollutants which act as potential EDs. In this study, the main purpose was to assess the possible estrogenic and thyrogenic activities of 32 commercial fish feeds. For the assessment of estrogenicity, a new estrogen receptor specific reporter gene assay using sea bass ERα (sbER α) was developed and validated. Potential thyroidal disruption was screened with a cell line permanently transfected with luciferase as reporter gene under the control of avian (av) thyroid receptor α (THRα). The results obtained showed that 11 and 18 out of 32 assayed feeds were able to activate the sbERα or the avTHRα1, respectively. The present study is pioneer in demonstrating thyrogenic activity in fish diets commercially available and widely used in aquaculture. Given that maintaining the homeostasis in the endocrine system is critical for the proper development and reproduction of fish, any estrogenic or thyrogenic activity caused by the feedstuffs should be taken into account with regards to its potential impact on farmed fish. Dr. Mosconi has always worked on fish physiology and in the last years has moved to the study of the interaction of pollutants with the reproductive physiology of fish.Maren Ortiz-Zarragoitia Dr. Professor, Zoology and Cell Biology, University of the Basque Country maren.ortiz@ehu.es Dr. Ortiz-Zarragoitia has a wide expertise in the field of ecotoxicology. Since the article we are sending deals with estrogenic and thyrogenic activities of fish feed, Dr Ortiz-Zarragoitia can act as reviewer due to his knowledge about the effect of pollutants on the endocrine system of fish. LAP Hoogenboom Dr. RIKILT Inst Food Safety ron.hoogenboom@wur.nl Dr. Hoogenboom has a broad expertise in the field of residue contaminants in feed and food. He has performed a variety of studies related with the detection of very different contaminants in feed, including estrogenic substances, growth-promoting agents, veterinary drugs and environmental dioxin-like substances.The estrogenicity and thyrogenic activity of fish feed extracts was assessed by means of cell lines containing the estrogen receptor or the thyroid receptor and reporter genes under the control of the corresponding receptor. For the assessment of estrogenic activities a new cell line containing the sea bass estrogen receptor has been developed. Although some estrogenic activity could be detected, strikingly a ve...

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“…Based on measured E2 concentrations in the current study (6 and 12 lg/L), the lack of significant vitellogenin induction (measured as whole-body vitellogenin) compared with the that of the controls (which likely represent baseline vitellogenin concentrations for male fish (Beresford et al 2011)) is consistent with previous studies with adult fathead minnows. One possible explanation is that the actual E2 concentrations that reached the livers in our test fish were not sufficient to induce (significant) vitellogenin expression greater than baseline concentrations.…”

Section: Discussionsupporting

confidence: 55%

Do Laboratory Species Protect Endangered Species? Interspecies Variation in Responses to 17β-Estradiol, a Model Endocrine Active Compound

Jorgenson

Buhl

Bartell

et al. 2014

Arch Environ Contam Toxicol

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Although the effects of estrogens on model laboratory species are well documented, their utility as surrogates for other species, including those listed as endangered, are less clear. Traditionally, conservation policies are evaluated based on model organism responses but are intended to protect all species in an environment. We tested the hypothesis that the endangered Rio Grande silvery minnow (Hybognathus amarus) is more vulnerable to endocrine disruption-as assessed through its larval predator-escape performance, survival, juvenile sex ratios, and whole-body vitellogenin concentration-than the commonly used toxicological model species fathead minnow (Pimephales promelas) and the bluegill sunfish (Lepomis macrochirus). Fish were exposed concurrently for 21 days to the model endocrine active compound (EAC) 17ß-estradiol (E2) at 10 ng E2/L and 30 ng E2/L in a flow-through system using reconstituted water that simulated the physicochemical conditions of the Middle Rio Grande in New Mexico, USA. No significant differences were observed between the fathead and silvery minnow in larval predator-escape response or juvenile sex ratio. Rio Grande silvery minnow survival decreased significantly at day 14 compared with the other two species; by day 21, both cyprinid species (silvery minnow and fathead minnow) exhibited a significant decrease in survival compared with bluegill sunfish, a member of the family Centrarchidae. Male Rio Grande silvery minnow showed a significant increase in whole-body vitellogenin concentration in the 10 ng/L treatment, whereas fathead minnow and bluegill sunfish showed no significant increases in vitellogenin concentrations across treatments. Our study showed response differences to estrogen exposures between the two cyprinid species and further divergence in responses between the families Cyprinidae and Centrarchidae. These results suggest that commonly used laboratory model organisms may be less sensitive to EACs than the endangered Rio Grande silvery minnow. However, this study supports the continued use of surrogate species for the beneficial implementation of water-quality regulations for the protection of threatened and endangered species if phylogenetic relationships are taken into consideration.

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Estrogenic activity of tropical fish food can alter baseline vitellogenin concentrations in male fathead minnow (Pimephales promelas)

Cited by 23 publications

References 41 publications

Burrowing in the Freshwater MusselElliptio complanatais Sexually Dimorphic and Feminized by Low Levels of Atrazine

Burrowing in the Freshwater MusselElliptio complanatais Sexually Dimorphic and Feminized by Low Levels of Atrazine

Assessment of estrogenic and thyrogenic activities in fish feeds

Do Laboratory Species Protect Endangered Species? Interspecies Variation in Responses to 17β-Estradiol, a Model Endocrine Active Compound

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