Development of a reproducible method to determine minimum inhibitory concentration (MIC) of plant extract against a slow-growing mycoplasmas organism

Abstract

Abstract

Antimycoplasmal activity of some plant species from northern Nigeria compared to the currently used therapeutic agent

“…Filter-sterilized amoxicillin-clavulanic acid was added at a concentration of 5 × 10 5 iu/L growth medium. One percent glucose and phenol red, which served as indicators for detection of the presence of Mycoplasma growth, was added to an aliquot of the enriched PPLO medium (Muraina et al, 2009).…”

“…To determine the minimum inhibitory concentration (MIC), a widely used method of two-fold serial microplate dilution was used with tetrazolium violet as an indicator of growth (Eloff, 1998b). This method did not work well, and a new method was developed (Muraina et al, 2009). The microplate was covered with thin aluminum foil to prevent light-associated damage to plant extracts and then incubated at 37°C for 24-36 h before the results were read by observation of color change of the reaction mixture from pinkish-red to yellow, indicating the presence of growth of metabolically active organisms (Muraina et al, 2009).…”

“…This method did not work well, and a new method was developed (Muraina et al, 2009). The microplate was covered with thin aluminum foil to prevent light-associated damage to plant extracts and then incubated at 37°C for 24-36 h before the results were read by observation of color change of the reaction mixture from pinkish-red to yellow, indicating the presence of growth of metabolically active organisms (Muraina et al, 2009). The minimum mycoplasmacidal concentration (MMC) of extracts with the lowest MICs was assessed by inoculating different concentrations of the extracts on PPLO agar and incubating for 3-10 days under a 5% carbon dioxide atmosphere to detect growth.…”

Chemical composition, antibacterial and antimycoplasma activities of four Eugenia species growing in Brazil

“…Infections caused by numerous microorganisms are a constant public health problem, and bacterial infections are responsible for several diseases such as pneumonia, meningitis, and endocarditis (Souza et al, 2004). Infections caused by resistant microorganisms and those caused by "commensal" microorganisms, are increasing every year (Muraina et al, 2009). Mycoplasmas are bacteria that belong to the class Mycoplasma, and cause respiratory and urogenital diseases in human beings (Muraina et al, 2009).…”

“…Infections caused by resistant microorganisms and those caused by "commensal" microorganisms, are increasing every year (Muraina et al, 2009). Mycoplasmas are bacteria that belong to the class Mycoplasma, and cause respiratory and urogenital diseases in human beings (Muraina et al, 2009). They are the smallest microorganisms that are capable of self-replication, lacking a cell wall and presenting variable susceptibility to antibiotics (Murray, 2007).…”