"Development of a rapid capillary zone electrophoresis method to quantify Levofloxacin and Meloxicam from transdermal therapeutic systems "

Abstract

Capillary zone electrophoresis (CZE) could be a useful technique for the quantification of active substances from transdermal therapeutic systems (TTSs). TTSs are pharmaceutical forms in development that may release one or more active substances with some significant advantages as increased compliance to treatment, avoidance of first hepatic passage and low manufacturing costs. A simple, reliable, efficient, and lowcost CZE method was developed and validated for the simultaneous determination of levofloxacin (fluoroquinolone) and meloxicam (non-steroidal anti-inflammatory) from TTSs. The selected experimental parameters were 50 mM borax (pH 9.3) as background electrolyte, +25 kV applied voltage, 50 mbar/5 seconds hydrodynamic injection and 40°C temperature, using an uncoated fused-silica capillary with (51 cm total length/43 cm effective length, 50 µm i.d.). CZE experiments were performed in less than four minutes with a resolution of 7.79 at a wavelength of 335 nm. Validation of the method presented good linearity data, precision (RDS% < 1 for migration times and RDS% < 2 for peaks area) and sensitivity (LOD 3.43 and 16.05 μg•mL-1 , LOQ 10.38 and 54.55 μg•mL-1 for levofloxacin and meloxicam, respectively). Recovery of the active substances ranged between 85.14% and 96.38%. Our developed CZE method proved its applicability for analysis of the two substances from TTSs.

Lamellar α-Zirconium Phosphate Nanoparticles Supported on N-Doped Graphene Nanosheets as Electrocatalysts for the Detection of Levofloxacin

“…Currently, various methods have been established for the detection of LEV, including methods based on fluorescence, , chemiluminescence, , capillary electrophoresis, , and high-performance liquid chromatography (HPLC). – Although these methods are reliable and widely accepted, they also have considerable shortcomings, such as being costly and time-consuming, in addition to requiring tedious operations, skilled technicians, and the consumption of large amounts of toxic solvents. Alternatively, electrochemical methods – have demonstrated outstanding advantages, such as reduced costs, simple operations, short response times, and high sensitivities.…”

Electrocatalytic Determination of the antibiotic Levofloxacin using modified carbon paste electrode with a poly‐murexide thin film voltammetrically

“…Thus, determining the LEVO amount has become necessary to maintain human health and protect the environment from pollution. Several accurate and sensitive analytical techniques have been extensively employed to determine LEVO, including spectrophotometry [6], high-performance liquid chromatography [7], enzyme-linked immune-sorbent assay [8], capillary electrophoresis [9], and Raman spectroscopy [10]. However, most of these techniques are complicated, requiring costly equipment and a long time for sample preparation.…”

Spectrophotometric and Multivariate Calibration Techniques for Simultaneous Determination of Different Drugs in Pharmaceutical Formulations and Human Urine: Evaluation of Greenness Profile

“…It is used for urinary, respiratory, skin, and soft tissue infections [33]. Several reports are found in the literature for the determination of LV including different techniques such as HPLC [34,35], spectrophotometry [34,36], voltammetry [37], and capillary electrophoresis [38].…”