Development of a one-step approach for the reconstruction of full thickness skin defects using minced split thickness skin grafts and biodegradable synthetic scaffolds as a dermal substitute

In Vitro and In Vivo Comparison Study of Electrospun PLA and PLA/PVA/SA Fiber Membranes for Wound Healing

“…Collagen is an integral component of healthy skin and is present in large doses in areas of tissue regeneration and cell proliferation [28]. Masson's staining results showed that on day 16, the collagens were intermittently found in the control group, indicating incomplete wound healing ( Figure 10A(a)), and the wound areas of dressing-treated groups ( Figure 10A(b,c)) showed relatively denser and more continuous collagen (blue staining).…”

“…IHC revealed that TNF-α levels in the PLA/PVA/SA groups were significantly lower than the control and PLA group on day 3, suggesting that PLA/PVA/SA dressings reduce inflammatory responses during early wound healing. This may be due to the anti-inflammatory and antibacterial activities of SA [28]. SA is biocompatible and highly hygroscopic, and is able to absorb wound exudate, maintaining a moist microenvironment to shorten the inflammatory phase of wound recovery and accelerate skin wound healing.…”

“…PVA is a synthetic but biocompatible polymer, and it is also being used widely for various medical applications as a medicinal excipient. It has been reported that in vitro synthetic electrospun PLA scaffolds can, as a dermal substitute, induce skin cell migration and proliferation along the fibers of the scaffold to form new collagen [28]. Nguyen et al produced curcumin-loaded PLA fibers and assessed their in vivo wound healing capability in rat models, displaying effective wound-healing performance [29].…”

Exploring treatments for ocular surface diseases

“…Electrospun scaffolds are being used for ophthalmology [102], skin [103], spinal cord [104] and bone [105]. With respect to limbal stem cell deficiency, electrospun scaffolds are being explored as an alternative to the amniotic membrane.…”

Investigation of Toxicological Properties of P(3HB-co-4HB) Electrospun Membranes as an Experimental Wound Dressings

“…Among the most promising cell scaffolds for derma restoration, nonwoven polymer membranes, produced by electrospinning, demonstrate better mechanical and biological properties, so as they have similar structure to that of mesh layer of derma, forming with the interlacing of collagen and elastin fibers (Sill, von Recum, 2008;Sharma et al, 2014 In accordance with the international systems of estimation of products, intended for the contact with the body and biological mediums, they must had no hazard effect and be examined with specific tests, depending on the purpose of the application. There are distinct requirements for toxicological safety for the medical materials, intended for prolonged direct contact with opened wound.…”