Development of a Novel Surface Porous Polymer Core-shell Ion-Exchange Filler and its Elution Behavior with Carbohydrates

Abstract

Shun-ichi Mitomo (Member), Yukiko Negishi (Non-member), Toshiki Mutai (Non-member)* and Yutaka Inoue (Non-member)** Abstract A novel core-shell ion-exchange filler composed of an ion-exchange porous shell layer formed on a hard polymer core was prepared for application to HPLC. We examined the effect of the thick shell of ion-exchange filler St-80 controlled by the weight ratio of the monomer for the core and shell (20:80) on the retention time and the theoretical plate number (N) in the separation of saccharides. A mixed aqueous sample of inositol, glucose, fructose and sucrose was well separated under alkaline conditions (100 and 150 mmol/L NaOH) at flow rates of 0.3-0.9 mL/min. The St-80 filler provides good chromatographic separation of saccharides, a good degree of separation between glucose and fructose and a high number of theoretical plates for saccharides.

Elution Behavior of Carbohydrates for Core-Shell Ion-Exchange Resin St-50 with Different Degrees of Cross-Linking in the Porous Shell

“…33 No.3 (2022) 63 also prepared using the same method (monomer ratio = 0:100) for comparison. Carbohydrate analysis via HPLC using St-80 (55%) demonstrated a shorter retention time compared with that demonstrated by the fully porous resin [28][29][30][31][32][33] . Core-shell ion-exchange resins (St-80) with a 20:80 core/shell monomer weight ratio were prepared for HPLC, and the effect of the degree of cross-linking in the porous shell (10, 40, and 55%) on the separation and theoretical plate number (N) was examined.…”

Elution Behavior of Carbohydrates for Core-Shell Ion-Exchange Resins with Different Degrees of Cross-Linking in Porous Shell Layer

“…The two important factors in the development of resins are the thickness [28][29][30] and the change in the degree of cross-linking in the porous layer. Therefore, it is important to clarify the appropriate combination of shell thickness and degree of cross-linking in the porous layer.…”

Development of Core-Shell Ion-Exchange Resin by Changing the Core-Shell Ratio and Its Elution Behavior with Carbohydrates

“…The HPLC analysis of carbohydrates for St-80 provided shorter retention time with similarly good resolution compared to the fully porous resin [17]. The porous shell layer and fully porous resin have the same degree of crosslinking (55%).…”

“…Therefore, we have prepared the core-shell ion-exchange resin (St-80) by controlling the weight ratio of the monomer for the core and the shell (20:80) and subsequent suspension polymerization as the other method [14][15][16][17]. The fully porous resin was also prepared by same method (monomer ratio = 0:100) for comparison with St-80.…”