Shun-ichi Mitomo (Member), Yukiko Negishi (Non-member), Toshiki Mutai (Non-member)* and Yutaka Inoue (Non-member)** Abstract A novel core-shell ion-exchange filler composed of an ion-exchange porous shell layer formed on a hard polymer core was prepared for application to HPLC. We examined the effect of the thick shell of ion-exchange filler St-80 controlled by the weight ratio of the monomer for the core and shell (20:80) on the retention time and the theoretical plate number (N) in the separation of saccharides. A mixed aqueous sample of inositol, glucose, fructose and sucrose was well separated under alkaline conditions (100 and 150 mmol/L NaOH) at flow rates of 0.3-0.9 mL/min. The St-80 filler provides good chromatographic separation of saccharides, a good degree of separation between glucose and fructose and a high number of theoretical plates for saccharides.
A novel core-shell ion-exchange resin (St-80) having a 20:80 weight ratio of the monomer for the core and the shell was prepared for use in high-performance liquid chromatography (HPLC), and the effect of the degree of cross-linking (10-55%) of the porous shell on the separation of carbohydrates was examined. A mixed aqueous sample of inositol, glucose, fructose, and sucrose was reasonably separated under strong alkaline conditions (0.10 and 0.15 mol/L NaOH eluent) at flow rates of 0.3-0.7 mL/min. As the degree of cross-linking in the shell portion increased, the retention time of sucrose, which had the longest elution time, decreased. Meanwhile, the theoretical plate number nearly doubled. The retention times obtained for the high degrees of cross-linking (40% and 55%) in the porous shell were shorter than that of the fully porous resin. The theoretical plate number observed for these resins provided excellent resolution, similar to that of the fully porous resin.
A novel core-shell ion-exchange resin composed of an ion-exchanging porous shell layer formed on a hard polymer core was prepared for application to HPLC. The effect of various core-shell ratios on the retention time and theoretical plate number (N) in the separation of carbohydrates was examined. A mixed aqueous sample of inositol, glucose, fructose, and sucrose was reasonably separated under alkaline conditions (100 and 150 mmol/L NaOH) at flow rates of 0.4-1.0 mL/min. The retention time was linearly related to the thickness of the porous layer. The values of theoretical plate number(N) of glucose, fructose, and sucrose depend on the shell thickness at a flow rate of 0.5 mL/min when using the 100 and 150 mmol/L NaOH eluent.
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