Development of a novel smartphone-based application for accurate and sensitive on-field hemoglobin measurement

Ghatpande, Niraj S.; Apte, Priti P.; Joshi, Bimba N.; Naik, Sadanand; Bodas, Dhananjay; Sande, Vishwajit; Uttarwar, Pravin; Kulkarni, Purushottam

doi:10.1039/c6ra24366e

Cited by 13 publications

(7 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of Hb detection show that our cell-phone-based acoustofluidic platform is portable and ready for POC testing, has a low requirement for sample volume (∼10 μL), and provides rapid (∼2 min) and easy readouts. Compared to previous methods, such as spectrophotometric measurement , and cell phone-assisted Hb quantitation, , the LOD of our method for Hb detection is much lower (Table S2). Moreover, our method is more biosafe than the Drabkin colorimetric assay (the international gold standard for Hb quantification), which employs highly toxic reagents (especially potassium cyanide) and generates highly hazardous waste.…”

Section: Resultsmentioning

confidence: 82%

A Cell-Phone-Based Acoustofluidic Platform for Quantitative Point-of-Care Testing

et al. 2020

View full text Add to dashboard Cite

Acoustofluidic methods, with advantages including simplicity of device design, biocompatible manipulation, and low power consumption, have been touted as promising tools for point-of-care (POC) testing. Here, we report a cellphone-based acoustofluidic platform that uses acoustic radiation forces to enrich nanoscale analytes and red and green fluorescence nanoparticles (SiO 2 @R and G@SiO 2 ) as probes for POC visual testing. Thus, the color signals from the fluorescent probes are enhanced, and colorimetric sensitivity is significantly improved. As a POC demonstration, the acoustofluidic platform is used to detect hemoglobin (Hb) from human blood, resulting in a rapid and straightforward measurement of normal blood Hb levels. Combining an acoustofluidic-based nanoparticle-concentration platform with cell-phone-based colorimetry, our method introduces a potential pathway toward practical POC testing.

show abstract

Section: Resultsmentioning

confidence: 82%

A Cell-Phone-Based Acoustofluidic Platform for Quantitative Point-of-Care Testing

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Smartphones offer an ideal POC platform, as they are already distributed widely worldwide and are in the hands of billions of users across the globe [18]. There has been emerging research on the potential role of smartphones to aid in the estimation of Hgb [19][20][21][22]. In particular, a novel, noninvasive smartphone application (app) described by Mannino, R.G., et al showed initial promising data on accurately measuring blood Hgb concentrations using only an unmodified smartphone [23].…”

Section: Introductionmentioning

confidence: 99%

Non-invasive hemoglobin measurement devices require refinement to match diagnostic performance with their high level of usability and acceptability

et al. 2021

View full text Add to dashboard Cite

Anemia remains an important global health problem. Inexpensive, accurate, and noninvasive solutions are needed to monitor and evaluate anemia in resource-limited settings. We evaluated the performance of multiple point-of-care hemoglobin devices, including a novel noninvasive smartphone application tested on Apple® and Android® cell phones, Masimo Pronto®, and HemoCue® Hb-301 and Hb-801, against a gold-standard hematology analyzer (reference hemoglobin) using venous blood. We examined correlations between hemoglobin devices and reference hemoglobin, device accuracy (average bias, Bland-Altman plots, clinical performance) and classification bias (sensitivity, specificity) among 299 refugees (10mo-65y) in Atlanta, GA. Semi-structured interviews (n = 19) with participants and staff assessed usability and acceptability. Mean reference hemoglobin was 13.7 g/dL (SD:1.8) with 12.5% anemia. Noninvasive hemoglobin devices were not well correlated with reference hemoglobin (Apple® R2 = 0.08, Android® R2 = 0.11, Masimo Pronto® R2 = 0.29), but stronger correlations were reported with HemoCue® Hb-301 (R2 = 0.87) and Hb-801 (R2 = 0.88). Bias (SD) varied across each device: Apple®: -1.6 g/dL (2.0), Android®: -0.7 g/dL (2.0), Masimo Pronto®: -0.4 g/dL (1.6), HemoCue® Hb-301: +0.4 g/dL (0.7) and HemoCue® Hb-801: +0.2 g/dL (0.6). Clinically acceptable performance (within ± 1 g/dL of reference hemoglobin) was higher for the invasive devices (HemoCue® Hb-301: 90.3%; HemoCue® Hb-801: 93.4%) compared to noninvasive devices (Apple®: 31.5%; Android®: 34.6%; Masimo Pronto®: 49.5%). Sensitivity and specificity were 63.9% and 48.2% for Apple®, 36.1% and 67.6% for Android®, 45.7% and 85.3% for Masimo Pronto®, 54.3% and 97.6% for HemoCue® Hb-301, and 66.7% and 97.6% for HemoCue® Hb-801. Noninvasive devices were considered easy to use and were the preferred method by participants. Among the only studies to compare multiple point-of-care approaches to hemoglobin testing, the diagnostic ability of HemoCue® was comparable to reference hemoglobin, while noninvasive devices had high user acceptability but considerable biases. Improvements in noninvasive device performance and further testing in anemic populations are recommended before broader use.

show abstract

“…Various biologically derived materials, such as enzymes, cells, nucleic acids, antigen–antibodies, and microorganisms, which could be tested on smartphones. Currently, many researchers have focused on how to combine smartphones and micro-biosensors, micro-bioelectronic mini-devices, microfluidic chips together to achieve real-time, point-of-care, and easy-to-use detections for analytes, especially for clinic and environmental samples [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…This has created unexpected opportunities for expanding the diagnostic and the prognostic approach to a large number of pathologies and for evaluating pharmaceutical therapies. With the aid of 3D-print technology, smartphone-based systems with 3D-printed cradles which were used to fix attachments have been explored in diagnostic field [ 8 , 9 , 10 ], food safety [ 11 , 12 ], environmental monitoring [ 13 ] and physical condition [ 3 , 14 ]. Though there are many different biosensor platforms based on smartphones, high-quality camera lenses on smartphones are a critical part and applied extensively for different smartphone biosensor platform.…”

Section: Introductionmentioning

confidence: 99%

Recent Progress in Optical Biosensors Based on Smartphone Platforms

Zhang

Fan

et al. 2017

Sensors

125

View full text Add to dashboard Cite

With a rapid improvement of smartphone hardware and software, especially complementary metal oxide semiconductor (CMOS) cameras, many optical biosensors based on smartphone platforms have been presented, which have pushed the development of the point-of-care testing (POCT). Imaging-based and spectrometry-based detection techniques have been widely explored via different approaches. Combined with the smartphone, imaging-based and spectrometry-based methods are currently used to investigate a wide range of molecular properties in chemical and biological science for biosensing and diagnostics. Imaging techniques based on smartphone-based microscopes are utilized to capture microscale analysts, while spectrometry-based techniques are used to probe reactions or changes of molecules. Here, we critically review the most recent progress in imaging-based and spectrometry-based smartphone-integrated platforms that have been developed for chemical experiments and biological diagnosis. We focus on the analytical performance and the complexity for implementation of the platforms.

show abstract

Development of a novel smartphone-based application for accurate and sensitive on-field hemoglobin measurement

Abstract: A smartphone-based app for the accurate and sensitive measurement of hemoglobin was developed.

Cited by 13 publications

References 22 publications

A Cell-Phone-Based Acoustofluidic Platform for Quantitative Point-of-Care Testing

A Cell-Phone-Based Acoustofluidic Platform for Quantitative Point-of-Care Testing

Non-invasive hemoglobin measurement devices require refinement to match diagnostic performance with their high level of usability and acceptability

Recent Progress in Optical Biosensors Based on Smartphone Platforms

Contact Info

Product

Resources

About