Development of a Novel Polymer-Based mRNA Coating for Surgical Suture to Enhance Wound Healing

Abstract

A therapeutic strategy to improve wound healing has become an increasingly important medical task due to the rising incidence of adiposity and type II diabetes as well as the proceeding population aging. In order to cope with the resulting burdens, new strategies to achieve rapid and complete wound healing must now be developed. Accordingly, the development of a bioactive wound dressing in the form of a messengerRNA (mRNA)-bearing poly(lactide-co-glycolide acid) (PLGA) coating on surgical suture is being pushed further with this study. Furthermore, the evaluation of the polymer-based transfection reagent Viromer RED has shown that it can be used for the transfection of eukaryotic cells: The mRNA gets properly complexed and translated into a functional protein.In addition, the mRNA-PLGA coating triggered the expression of the keratinocyte growth factor (KGF) in HaCat cells although KGF is not expressed under physiological conditions. Moreover, transfection via surgical sutures coated with mRNA does not affect the cell viability and a proinflammatory reaction in the transfected cells is not induced. These properties make the mRNA-PLGA coating very attractive for the in vivo application. For the future, this could mean that through the use of mRNA-coated sutures in surgical wound closure, cells in the wound area can be transfected directly, thus accelerating and improving wound healing.

Surgical suture material—fundamentals

“…Bei der Auswahl des Nahtmaterials gilt der Grundsatz, mit einem Minimum an unerwünschten Gewebereaktionen und infektiösem Potenzial eine ausreichende Festigkeit der Wunde

Festigkeit der Wunde

für die notwendige Dauer der Wundheilung zu erreichen. Stärkeres oder zugfesteres Nahtmaterial ist jedoch nicht immer besser, da das Fadenkaliber erhöht werden muss und es zu einer unbeabsichtigten Strangulierung des Gewebes kommen kann, was einerseits die Durchblutung gefährdet, möglicherweise auch die Entzündungsreaktivität erhöht [ 10 ]. Die einzelnen Gewebe, Schichten und Indikationen erfordern Konditionen von der Sehnenreparatur bis zur feinen kosmetischen Naht im Gesicht (Abb.…”

“…Experimentelle Untersuchungen lassen für die Zukunft die Entwicklung von mRNA-beschichtetem Nahtmaterial beim chirurgischen Wundverschluss in den Fokus rücken. Zellen im Wundbereich könnten direkt übertragen werden, wodurch die Wundheilung beschleunigt und verbessert werden könnte [ 10 ].…”

Harnessing Multifaceted Next-Generation Technologies for Improved Skin Wound Healing

“…Multiple strategies have been developed for gene/mRNA-based growth factor delivery. 132,133 Nucleotide-based growth factor delivery strategies have been further discussed in section 4.5.…”

“…This platform delivers the gene/mRNA (encoding the desired protein) inside the host cells at a wound site, which then translates the corresponding protein/growth factor and increases its availability in the local microenvironment. Multiple strategies have been developed for gene/mRNA-based growth factor delivery. , Nucleotide-based growth factor delivery strategies have been further discussed in section .…”

Enhancing Dental Applications: A Novel Approach for Incorporating Bioactive Substances into Textile Threads

“…Post immersion, excess solution removal can be accomplished through mechanical squeezing [10] or drying procedures [11]. Alternatively, more sophisticated methods are available, including non-contact deposition techniques like drop coating [12], spray coating [13][14][15], ink-jet coating or printing [16,17], coaxial nozzle coating [18], plasma and ionized gas deposition [19], or electrostatic spinning [20][21][22]. Furthermore, active agents can be introduced into the textile yarns through contact deposition, employing coating principles [23].…”