Development of a new clinically applicable device for embryo evaluation which measures embryo oxygen consumption

Kurosawa, Hiroki; Utsunomiya, Hiroki; Shiga, Naomi; Takahashi, Aiko; Ihara, Masataka; Ishibashi, Masayoshi; Nishimoto, Masashi; Watanabe, Zen; Abé, Hiroyuki; Kumagai, Jin; Igarashi, Hideki; Takahashi, Toshifumi; Fukui, Atsushi; Suganuma, Ryota; Tachibana, Masahito; Yaegashi, Nobuo

doi:10.1093/humrep/dew187

Cited by 45 publications

(45 citation statements)

References 40 publications

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“…The existing methods from the 1990s for measuring oxygen consumption failed to become a gold standard due to their lack of high sensitivity coupled by their invasive nature. Another innovative method, scanning electrochemical microscopy, employed to measure the oxygen consumption rate failed to excite embryologists as reported by Kurosawa et al due to the fact that it requires micromanipulation and further training (73). Finally, a new device developed by Kurosawa et al that included a chip sensing embryo respiration monitoring system achieved an automated measurement of oxygen consumption rate in a satisfactory fashion (73).…”

Section: Latest Trendsmentioning

confidence: 99%

“…Another innovative method, scanning electrochemical microscopy, employed to measure the oxygen consumption rate failed to excite embryologists as reported by Kurosawa et al due to the fact that it requires micromanipulation and further training (73). Finally, a new device developed by Kurosawa et al that included a chip sensing embryo respiration monitoring system achieved an automated measurement of oxygen consumption rate in a satisfactory fashion (73). No matter how exciting these latest trends sound, usability within the clinical spectrum of the IVF laboratory is a required condition that should be seriously accounted for.…”

Section: Latest Trendsmentioning

confidence: 99%

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Considerations Regarding Embryo Culture Conditions: From Media to Epigenetics

Simopoulou

Sfakianoudis²,

Rapani

et al. 2018

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Section: Latest Trendsmentioning

confidence: 99%

Section: Latest Trendsmentioning

confidence: 99%

Considerations Regarding Embryo Culture Conditions: From Media to Epigenetics

Simopoulou

Sfakianoudis²,

Rapani

et al. 2018

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show abstract

“…[43][44][45] In one such device, a single mouse embryo was positioned in a microwell surrounded by electrodes (Fig. 8), and the O 2 consumption of the two-cell, morula, and blastocyst stages was successfully measured.…”

Section: Electrode Array Devicesmentioning

confidence: 99%

Micro/nanoelectrochemical probe and chip devices for evaluation of three-dimensional cultured cells

Ino

Şen

Shiku

et al. 2017

Analyst

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Herein, we present an overview of recent research progress in the development of micro/nanoelectrochemical probe and chip devices for the evaluation of three-dimensional (3D) cultured cells. First, we discuss probe devices: a general outline, evaluation of O 2 consumption, enzyme-modified electrodes, evaluation of endogenous enzyme activity, and the collection of cell components from cell aggregates are discussed. The next section is focused on integrated chip devices: a general outline, electrode array devices, smart electrode array devices, droplet detection of 3D cultured cells, cell manipulation using dielectrophoresis (DEP), and electrodeposited hydrogels used for fabrication of 3D cultured cells on chip devices are discussed. Finally, we provide a summary and discussion of future directions of research in this field.

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“…The “quiet” metabolism of embryos shifts from oxidative phosphorylation to glycolysis during the morula and blastocyst stages—particularly in cells of the inner cell mass, whereas trophoblasts consume much more oxygen (Houghton, ; reviewed by Gardner and Harvey, ). This metabolic characteristic, first discovered by Otto Warburg in tumors (Warburg, ), is shared by proliferating cells (Heiden, Cantley, & Thompson, ; Lunt & Vander Heiden, ) and persists through later embryonic stages (Krisher & Prather, ; Redel et al, ; Smith & Sturmey, ), although the increasing population of oxidative phosphorylation‐utilizing trophoblasts could account for the net increase in oxygen consumption reported between the morula and blastocyst stages (Kurosawa et al, ). This shift to glycolytic metabolism in the inner cell mass is not a result of anaerobic glycolysis, however, because embryonic stem cells, derived from the inner cell mass (Evans & Kaufman, ), also exhibit high rates of glycolysis over oxidative phosphorylation when they are cultured in high oxygen‐partial pressure conditions (Turner et al, ).…”

Section: Embryo Development and The In Vivo Environmentmentioning

confidence: 99%

Metabolite availability as a window to view the early embryo microenvironment in vivo

2017

Molecular Reproduction Devel

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A preimplantation embryo exists independent of blood supply, and relies on energy sources from its in vivo environment (e.g., oviduct and uterine fluid) to sustain its development. The embryos can survive in this aqueous environment because it contains amino acids, proteins, lactate, pyruvate, oxygen, glucose, antioxidants, ions, growth factors, hormones, and phospholipids-albeit the concentration of each component varies by species, stage of the estrous cycle, and anatomical location. The dynamic nature of this environment sustains early development from the one-cell zygote to blastocyst, and is reciprocally influenced by the embryo at each embryonic stage. Focusing on embryo metabolism allowed us to identify how the local environment was deliberately selected to meet the dynamic needs of the preimplantation embryo, and helped reveal approaches to improve the in vitro culture of human embryos for improved implantation rates and pregnancy outcome. | INTRODUCTIONThe basic energy needs-for example, glucose, pyruvate, and lactateof mammalian early embryos were determined for their in vitro culture nearly half a century ago (Biggers & Stern, 1973;Biggers, Whittingham, & Donahue, 1967), with the impact of endogenous fuels, including lipids and amino acids, identified soon thereafter (Chatot, Tasca, & Ziomek, 1990;Hillman & Flynn, 1980;Lane & Gardner, 1998;Quinn & Whittingham, 1982). Considerable progress has been made since these first studies, specifically defining a relatively complete system that accounts for the embryo's changing metabolism, which allowed for better reproducibility of in vitro culture as well as non-invasive assessment of embryo quality (Gardner & Leese, 1986;Gardner & Wale, 2013;Prasad, Tiwari, Koch, & Chaube, 2015;Houghton et al., 2002;Leese, Conaghan, Martin, & Hardy, 1993;Smith & da Rocha, 2012;Thompson, Brown, & Sutton-McDowall, 2016;Vajta, Rienzi, Cobo, & Yovich, 2010). Unfortunately, much of the data on embryo metabolism has come from in vitro studies (AbsalonMedina, Butler, & Gilbert, 2014;Gardner and Harvey, 2015;Leese, 2012Leese, , 2015Menezo, Lichtblau, & Elder, 2013

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Development of a new clinically applicable device for embryo evaluation which measures embryo oxygen consumption

Abstract: Not applicable.

Cited by 45 publications

References 40 publications

Considerations Regarding Embryo Culture Conditions: From Media to Epigenetics

Considerations Regarding Embryo Culture Conditions: From Media to Epigenetics

Micro/nanoelectrochemical probe and chip devices for evaluation of three-dimensional cultured cells

Metabolite availability as a window to view the early embryo microenvironment in vivo

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