Development of a needle shaped microelectrode for electrochemical detection of the sepsis biomarker interleukin-6 (IL-6) in real time

Russell, Christopher; Ward, Andrew; Vezza, Vincent; Hoskisson, Paul A.; Alcorn, David; Steenson, D.P.; Corrigan, Damion K.

doi:10.1016/j.bios.2018.11.053

Cited by 154 publications

(103 citation statements)

References 34 publications

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“…This is analogous to direct enzyme linked immunoassay (ELISA) approach which produces fluorescence when the antigen and antibody complexes are formed. Antibodies against IL-6 can be directly immobilized on the surface of a functionalized electrode [100] or nano-features can be introduced in the sensing layer [101][102][103] for signal amplification purposes as shown in Figure 2. A robust dECIA method with a wide linear range was demonstrated by Tertis et al [101], and aptamer against IL-6 was immobilized on polypyrrole nanoparticles (PPy) decorated with gold nanoparticles (AuNPs) of 50 nm diameter.…”

Section: Direct Electrochemical Immunoassay (Decia)mentioning

confidence: 99%

“…Different sensing mechanisms developed for IL-6 detection. Direct electrochemical immunoassay (ECIA) based on IL-6 and the primary antibody (Ab 1 ) interaction [100]; using a conjugate of polypyrrole-Au Nanoparticles-aptamer [101]; carbon nanotubes (CNTs) coated with (Ab 1 ) [102]; carbon nanotubes coated with gold nanoparticles (AuNPs) and (Ab 1 ) [103]. Indirect electrochemical immunoassay in which a competitive antigen bound to AgNP-coated Polystyrene nanobeads [104].…”

Section: Direct Electrochemical Immunoassay (Decia)mentioning

confidence: 99%

“…The geometry of the electrode's surface plays a pivotal role in preventing the counter-ions screening at the double layer [123], and a concave surface promotes efficient electron transfer kinetics, thereby increasing the sensitivity of the sensor. An array of eight gold disc-shaped microelectrodes was engraved on a silicon needle with an objective of real-time in-situ monitoring of IL-6 [100] ( Figure 4A). This approach was envisioned following the development of a robust microelectrode-based electrochemical sensing platform for chemical and DNA sensing applications [124][125][126][127].…”

Section: Electrode Interfacementioning

confidence: 99%

“…Different schemes of sensing layer, as discussed earlier, were proposed with an aim to amplify the readout signal which ultimately improves sensitivity and dynamic range of biosensor. Some of the effective approaches which significantly boost the outgoing signals of sensor are (1) modification of electrode geometry and size [100]; (2) coating of electrode with nano-features, such as SWCNT, AuNP, and Polypyrrole nanoparticles [101][102][103][104]; and (3) labeling of conjugate in sandwich configuration with nanoparticles, enzymes or a combination of two [105,106,[108][109][110] (Figure 4A). Figure 4B shows a comparison of intensity of readout signal against the least concentration detected by each configuration, i.e., direct and sandwich electrochemical immunoassay.…”

Section: Signal Amplificationmentioning

confidence: 99%

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Recent Advances in Electrochemical and Optical Biosensors Designed for Detection of Interleukin 6

Khan

Mujahid

2020

Sensors

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Interleukin 6 (IL-6), being a major component of homeostasis, immunomodulation, and hematopoiesis, manifests multiple pathological conditions when upregulated in response to viral, microbial, carcinogenic, or autoimmune stimuli. High fidelity immunosensors offer real-time monitoring of IL-6 and facilitate early prognosis of life-threatening diseases. Different approaches to augment robustness and enhance overall performance of biosensors have been demonstrated over the past few years. Electrochemical- and fluorescence-based detection methods with integrated electronics have been subjects of intensive research due to their ability to offer a better signal-to-noise ratio, high specificity, ultra-sensitivity, and wide dynamic range. In this review, the pleiotropic role of IL-6 and its clinical significance is discussed in detail, followed by detection schemes devised so far for their quantitative analysis. A critical review on underlying signal amplification strategies and performance of electrochemical and optical biosensors is presented. In conclusion, we discuss the reliability and feasibility of the proposed detection technologies for commercial applications.

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Section: Direct Electrochemical Immunoassay (Decia)mentioning

confidence: 99%

Section: Direct Electrochemical Immunoassay (Decia)mentioning

confidence: 99%

Section: Electrode Interfacementioning

confidence: 99%

Section: Signal Amplificationmentioning

confidence: 99%

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Recent Advances in Electrochemical and Optical Biosensors Designed for Detection of Interleukin 6

Khan

Mujahid

2020

Sensors

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“…Nevertheless, progress has been made with the development of biosensors that can be incorporated into these devices for the measurement of biomarkers such as PCT [124]. Recent work has led to the development of a microelectrode sensor for the measurement of IL-6 with an assay time of around 2.5 min [125]. Work on more novel biomarkers has also been done by Min and co-workers [126], who developed a biosensor for the measurement of IL-3 and have attempted to validate its use in a small cohort of septic and control patients.…”

Section: Point-of-care Testing (Poct) For Sepsismentioning

confidence: 99%

Biomarkers for Point-of-Care Diagnosis of Sepsis

2020

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Sepsis is defined as a life-threatening organ dysfunction caused by a dysregulated host response to infection. In 2017, almost 50 million cases of sepsis were recorded worldwide and 11 million sepsis-related deaths were reported. Therefore, sepsis is the focus of intense research to better understand the complexities of sepsis response, particularly the twin underlying concepts of an initial hyper-immune response and a counter-immunological state of immunosuppression triggered by an invading pathogen. Diagnosis of sepsis remains a significant challenge. Prompt diagnosis is essential so that treatment can be instigated as early as possible to ensure the best outcome, as delay in treatment is associated with higher mortality. In order to address this diagnostic problem, use of a panel of biomarkers has been proposed as, due to the complexity of the sepsis response, no single marker is sufficient. This review provides background on the current understanding of sepsis in terms of its epidemiology, the evolution of the definition of sepsis, pathobiology and diagnosis and management. Candidate biomarkers of interest and how current and developing point-of-care testing approaches could be used to measure such biomarkers is discussed.

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Sensing Inflammation Biomarkers with Electrolyte‐Gated Organic Electronic Transistors

Burtscher

Urbina

Diacci

et al. 2021

Adv Healthcare Materials

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An overview of cytokine biosensing is provided, with a focus on the opportunities provided by organic electronic platforms for monitoring these inflammation biomarkers which manifest at ultralow concentration levels in physiopathological conditions. Specifically, two of the field's state-of-the-art technologies-organic electrochemical transistors (OECTs) and electrolyte gated organic field effect transistors (EGOFETs)-and their use in sensing cytokines and other proteins associated with inflammation are a particular focus. The overview will include an introduction to current clinical and "gold standard" quantification techniques and their limitations in terms of cost, time, and required infrastructure. A critical review of recent progress with OECT-and EGOFET-based protein biosensors is presented, alongside a discussion onthe future of these technologies in the years and decades ahead. This is especially timely as the world grapples with limited healthcare diagnostics during the Coronavirus disease (COVID-19)pandemic where one of the worst-case scenarios for patients is the "cytokine storm." Clearly, low-cost point-of-care technologies provided by OECTs and EGOFETs can ease the global burden on healthcare systems and support professionals by providing unprecedented wealth of data that can help to monitor disease progression in real time.

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Development of a needle shaped microelectrode for electrochemical detection of the sepsis biomarker interleukin-6 (IL-6) in real time

Cited by 154 publications

References 34 publications

Recent Advances in Electrochemical and Optical Biosensors Designed for Detection of Interleukin 6

Recent Advances in Electrochemical and Optical Biosensors Designed for Detection of Interleukin 6

Biomarkers for Point-of-Care Diagnosis of Sepsis

Sensing Inflammation Biomarkers with Electrolyte‐Gated Organic Electronic Transistors

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