Development of a multiwalled carbon nanotube coated collagen dish

Abstract

Carbon nanotubes (CNTs) are one of the most interesting nanomaterials because of their excellent characteristics. In this study, a transparent CNTs coating for cell culture dishes was developed and its properties for cell culture were estimated. Carboxylated multiwalled carbon nanotubes (MWCNTs) were dispersed in aqueous sodium cholate solution and applied on a collagen type I-coated cell culture dish (cover glass). The dish surface was homogeneously covered by MWCNTs without aggregation. The MWCNT-coated dish was slightly gray and had good transparency, so conventional optical microscopic observation of the cells on the MWCNT-coated dish was possible. Rat osteoblast-like cells cultured on the MWCNT-coated dish showed slightly lower viability and proliferation compared to the collagen-coated dish. The cell adhesion on the MWCNT-coated dish was much higher than that on the collagen-coated dish. Therefore, MWCNT-coating for dishes will be a useful new material for cell culture.

Carbon nanotube coating on titanium substrate modified with TiO2 nanotubes

“…Some filopodia protruding from cells seemed to make contact with CNTs, which would assist in the improvement of cell adhesion as shown in Fig.5(c). Some previous studies also reported that the strong cell adhesion was suggested to be due to the mechanical interaction between CNTs and the cellsurface filopodia [4,11] . CNTs are loaded on the TiO 2 NT substrate, which not only being benefi cial for cell adhesion but also provides excellent conductivities that provide more efficient transportation of energies and substances, owing to their aromatic graphite structure.…”

Temporomandibular Disorders and BIO-IPNs: in vitro approach to find molecular solution to biological problem

“…In particular, bone tissue compatibility is extremely important for using CNTs in biomaterials. Usui et al, who first developed CNT, found that these tubes have good bone tissue compatibility and are capable of permitting bone repair and becoming closely integrated with bone tissue and accelerate bone formation stimulated by recombinant human bone morphogenetic protein-2 [150].…”

Hydrogels based on collagen and fibrin – frontiers and applications

“…They are rolled-up graphite sheets building single-walled (SWCNT) or multi-walled (MWCNT) hollow cylinders [61]. CNTs are interesting materials because of their unique mechanical and electrical properties [62]. They may be used as scaffolds for cardiac or neural tissue [63].…”