Development of a multiplex assay for the detection of antibodies to Borrelia burgdorferi in horses and its validation using Bayesian and conventional statistical methods

Comparison of Immunologic Responses Following Intranasal and Oral Administration of a USDA-Approved, Live-Attenuated Streptococcus equi Vaccine

“…OspC antibodies were found during early infection and OspF antibodies were found with chronic infection (Wagner et al 2011). OspA antibodies are found with vaccination as in humans.…”

“…Microsphere immunoassays have been developed to detect serologic responses to a variety of equine diseases including West Nile virus (WNV) (Balasuriya et al 2006), flavivirusassociated neurologic diseases , equine viral arteritis (Go et al 2008), African horse sickness (Sanchez-Matamoros et al 2015), Lyme disease (Wagner et al 2011), andHendra virus (McNabb et al 2014). An MIA incorporating recombinant WNV envelope protein (rE) was found to have diagnostic sensitivity and specificity (99.3% and 97.4%, respectively) compared to plaque reduction neutralization test (PRNT) (Balasuriya et al 2006).…”

“…A multiplex MIA utilizing three outer surface proteins (Osp) of Borrelia burgdorferi was developed to determine vaccination versus early or chronic infection (Wagner et al 2011).…”

“…Compared to the "relative" gold standard of WB, the multiplex MIA had improved sensitivity with antibody detection by the MIA in pg/ml compared to the WB in µg/ml (Wagner et al 2011). The MIA provided a quantitative measurement compared to WB which would be useful for treatment response; and the MIA is more specific than quantitative ELISA tests.…”

Lyme borreliosis in the horse: A mini-review

“…This assay has improved analytical and diagnostic sensitivities compared to Western blot analysis. Multiplex analysis is a valuable quantitative tool that simultaneously detects antibodies indicative for natural infection with and/or vaccination against the Lyme pathogen (Wagner et al, 2011).…”