Development of a multi-component analysis system

Villanueva, Victor R.; Mardon, M.; Goff, M.-T. Le; Moncelon, F.

doi:10.1016/s0021-9673(01)94207-4

Cited by 13 publications

(2 citation statements)

References 19 publications

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“…The post-column derivatization methods previously reported for methylated arginines require complicated gradient profile and long separation time of more than 80 min. 18,19 It should be noted that, as far as we know, cyclohexanecarboxylic acid has not been used as an ion-pair reagent and is not available in the market in a pure form, thus it must be entirely purified before use as described in the experimental section.…”

Section: Separation Of Methylated Argininesmentioning

confidence: 99%

“…Also, although the post-column derivatization method with OPA and thiol after separation on ion-exchange chromatography made it possible to avoid such tedious pre-treatment procedures, it requires complicated gradient profiles and a long separation time ( > 80 min). 18,19 Nevertheless, the methylated arginines were still not satisfactorily separated.…”

Section: Introductionmentioning

confidence: 99%

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An automated analyzer for methylated arginines in rat plasma by high-performance liquid chromatography with post-column fluorescence reaction

Dobashi

Santa

Nakagomi

et al. 2001

Analyst

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A fully automated analyzer for methylated L-arginine metabolites [N,N-dimethyl-L-arginine (ADMA), N-methylarginine (NMMA) and N,N'-dimethyl-L-arginine (SDMA)] by high-performance liquid chromatography with post-column fluorescence derivatization was developed. This system consists of an on-line extraction, a separation on a reversed phase ion-pair chromatograph, a post-column derivatization by o-phthaladehyde (OPA) and thiol reaction, and fluorescence detection. NMMA, ADMA and SDMA were separated in 40 min with isocratic elution by a combination of octanoate and cyclohexane carboxylate as ion-pair reagents. The eluate was monitored at 450 nm with excitation at 337 nm. The calibration curves for NMMA, ADMA and SDMA showed linearity over the range from 0.05 micromol l(-1) (0.5 pmol on column) to 5.0 micromol l(-1) (50 pmol on column). This method does not require any time-consuming pre-treatment and requires only 10 microl of plasma sample for assay.

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Section: Separation Of Methylated Argininesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An automated analyzer for methylated arginines in rat plasma by high-performance liquid chromatography with post-column fluorescence reaction

Dobashi

Santa

Nakagomi

et al. 2001

Analyst

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Contribution to Forest Decline Studies; Biochemical Effects of Atmospheric Pollution (Acid Rain) in Picea Trees

Villanueva

Santerre

1991

Acid Deposition

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Amino acids, polyamines and proteins during seed germination of two species of Dipterocarpaceae

Huang

Villanueva

1993

Trees

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Summary. Amino acid, polyamine and protein concentrations in seeds and their evolution during seed germination of two dipterocarp species, Hopea odorata and Dipterocarpus alatus, were determined with the help of a multianalytical system. Glutamic acid and glutamine were the major amino compounds present. Hopea seeds also contain high levels of aspartic acid/asparagine, serine, threonine, arginine and alanine, while those of Dipterocarpus contain high levels of alanine, arginine and threonine. These species were quite different in their germination behavior and thus in their protein and amine metabolism rates. In Hopea, polyamines increased during the first 3 days of germination and reached a maximum by the 3rd day, 1 day before maximum germination rate. In Dipterocarpus polyamines reached their maximum at the 6th day while maximum germination rate is observed by the 7th day. This suggests that polyamine compounds could play a role in the early part of the germination process in Hopea and Dipterocarpus seeds. The possibility that control of polyamine biosynthesis could be used for the establishment of biochemical methods to improve seed storage and to control germination of these recalcitrant seeds is discussed.

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Development of a multi-component analysis system

Cited by 13 publications

References 19 publications

An automated analyzer for methylated arginines in rat plasma by high-performance liquid chromatography with post-column fluorescence reaction

An automated analyzer for methylated arginines in rat plasma by high-performance liquid chromatography with post-column fluorescence reaction

Contribution to Forest Decline Studies; Biochemical Effects of Atmospheric Pollution (Acid Rain) in Picea Trees

Amino acids, polyamines and proteins during seed germination of two species of Dipterocarpaceae

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