An automated analyzer for methylated arginines in rat plasma by high-performance liquid chromatography with post-column fluorescence reaction

Dobashi, Yoshinori; Santa, Tomofumi; Nakagomi, Kazuya; Imai, Kazuhiro

doi:10.1039/b106828h

Cited by 30 publications

(19 citation statements)

References 25 publications

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“…These methods require tedious sample preparation with cation-exchange solid-phase extraction. A fully automated version of such a procedure including sample preparation is presented by Dobashi et al [15], employing a very complicated system with several pumps and switching valves, which is not easily set up in all laboratories. An HPLC method comprising OPA derivatization and fluorescence detection without sample pretreatments other than protein precipitation is presented by Chen et al [16], but presumably they have not achieved separation of ADMA from SDMA.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous detection of arginine, asymmetric dimethylarginine, symmetric dimethylarginine and citrulline in human plasma and urine applying liquid chromatography–mass spectrometry with very straightforward sample preparation

Martens‐Lobenhoffer

Bode‐Böger

2003

Journal of Chromatography B

102

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Section: Introductionmentioning

confidence: 99%

Simultaneous detection of arginine, asymmetric dimethylarginine, symmetric dimethylarginine and citrulline in human plasma and urine applying liquid chromatography–mass spectrometry with very straightforward sample preparation

Martens‐Lobenhoffer

Bode‐Böger

2003

Journal of Chromatography B

102

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“…Several HPLC methods have been reported for the analysis of ADMA [9,17,18]. The HPLC methods utilize several detection techniques, but the most common method for ADMA analysis has been fluorescence detection with ortho-phthaldialdehyde (OPA) or naphthalene-2,3-dicarboxaldehyde (NDA) derivatization due to their high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Comparison of HPLC method and commercial ELISA assay for asymmetric dimethylarginine (ADMA) determination in human serum

Valtonen

Karppi

Nyyssönen

et al. 2005

Journal of Chromatography B

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“…The plasma concentration of ADMA was measured by high-performance liquid chromatography (HPLC) with a fluorescent detection method, 22 using blood samples that had been collected in EDTA tubes. The plasma was separated and 10 l was added to 40 l of mobile phase solution for HPLC.…”

Section: Measurement Of Admamentioning

confidence: 99%

Amelioration of Vascular Endothelial Dysfunction in Obstructive Sleep Apnea Syndrome by Nasal Continuous Positive Airway Pressure-Possible Involvement of Nitric Oxide and Asymmetric NG, NG-Dimethylarginine-

Ohike

Kozaki

Iijima

et al. 2005

Circ J

Self Cite

145

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ndothelial dysfunction is recognized as an early phase of arteriosclerosis 1 and an important cause of that dysfunction is impaired nitric oxide (NO) release from the endothelium. Endothelial NO is a key regulator of vascular homeostasis; it induces vasorelaxation by generating cyclic GMP in the underlying smooth muscle cells, and prevents monocyte adhesion to the endothelium, platelet activation, and smooth muscle cell proliferation. Hence, impaired NO release from injured endothelial cells is regarded as an initiator and promoter of arteriosclerosis.Endothelial NO is produced when L-arginine is con- February 2005 verted to L-citrulline by the enzyme endothelial nitric oxide synthase (eNOS). Endothelial NOS is inhibited by endogenous inhibitors, NG-monomethyl-L-arginine (L-NMMA) and asymmetric dimethylarginine (ADMA), which are structural analogues of L-arginine. 2,3 Plasma ADMA is eliminated by renal excretion and by degradation to citrulline and dimethylamine by the enzyme dimethylarginine dimethylaminohydrolase (DDAH). 4 Increased plasma concentration of ADMA is associated with hypertension, 5 pulmonary hypertension, 6 hypercholesterolemia, 7,8 carotid intima -media thickening, 9 severe peripheral artery occlusive disease, 10 and the clustering of coronary risk factors. 9 These findings suggest that ADMA is responsible for endothelial dysfunction. Obstructive sleep apnea syndrome (OSAS) has been recently attracting attention as a significant disorder. Frequent apnea/hypopnea attacks followed by arousal results in insufficient sleep at night, causing daytime sleepiness, leading to work inefficiency, and even traffic accidents. In addition, OSAS often accompanies hypertension, obesity, glucose intolerance, and dyslipidemia, all of which are factors in metabolic syndrome. Hence, OSAS is recognized as a risk factor for cardiovascular disease. 11-14 It has been Background Asymmetric NG,NG-dimethylarginine (ADMA) is an endogenous inhibitor of endothelial nitric oxide (NO) synthase and its plasma concentration is elevated in patients with cardiovascular risk factors, including hyperlipidemia, hypertension, diabetes, and hyperhomocysteinemia. Obstructive sleep apnea syndrome (OSAS) has been attracting attention as a risk factor for cardiovascular disorders because it often accompanies hypertension, obesity, glucose impairment, and dyslipidemia, all of which are factors in metabolic syndrome and risk factors for cardiovascular disease. Methods and ResultsIn the present study, flow-mediated vasodilatation (FMD) of the brachial artery and plasma concentrations of ADMA were measured before and after nasal continuous positive airway pressure (nCPAP) therapy, which abrogates apnea, in 10 male patients aged 36-69 years old, who were given a diagnosis of OSAS by polysomnography. The percent FMD (%FMD) improved significantly from 3.3±0.3% to 5.8±0.4% (p<0.01) and 6.6±0.3% (p<0.01), before, 1 week, and 4 weeks after nCPAP, respectively. At the same time, the plasma NOx concentrations, metabolites of NO, tended to increase, bu...

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An automated analyzer for methylated arginines in rat plasma by high-performance liquid chromatography with post-column fluorescence reaction

Cited by 30 publications

References 25 publications

Simultaneous detection of arginine, asymmetric dimethylarginine, symmetric dimethylarginine and citrulline in human plasma and urine applying liquid chromatography–mass spectrometry with very straightforward sample preparation

Simultaneous detection of arginine, asymmetric dimethylarginine, symmetric dimethylarginine and citrulline in human plasma and urine applying liquid chromatography–mass spectrometry with very straightforward sample preparation

Comparison of HPLC method and commercial ELISA assay for asymmetric dimethylarginine (ADMA) determination in human serum

Amelioration of Vascular Endothelial Dysfunction in Obstructive Sleep Apnea Syndrome by Nasal Continuous Positive Airway Pressure-Possible Involvement of Nitric Oxide and Asymmetric NG, NG-Dimethylarginine-

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