Development of a highly sensitive and specific monoclonal antibody based enzyme‐linked immunosorbent assay for the detection of a new β‐agonist, phenylethanolamine A, in food samples

Jiang, Dongliang; Cao, Biyun; Yang, Hong; Zhao, Kang; Li, Jianguo; Li, Mingxin; Sun, Lulu; Deng, Anping

doi:10.1002/jsfa.7826

Cited by 17 publications

(5 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Immunological and instrumental methods are currently the primary analytical methods for β-agonists in animal-derived foods. Enzyme-linked immunosorbent assays (ELISA) [9] and colloidal gold immunochromatography assays (GICA) [10] are examples of immunological techniques. Instrumental methods include capillary electrophoresis (CE) [11], gas chromatography-tandem mass spectrometry (GC-MS/MS) [12], liquid chromatography-tandem mass spectrometry (LC-MS/MS) [13].…”

Section: Introductionmentioning

confidence: 99%

Rapid and High-Throughput Determination of Sixteen β-agonists in Livestock Meat Using One-Step Solid-Phase Extraction Coupled with UHPLC-MS/MS

Yan

Ning

Cheng

et al. 2022

Foods

View full text Add to dashboard Cite

β-agonists are illegally added to animal feed because they can greatly increase carcasses’ leanness, which impairs the safety of animal-derived foods and indirectly endangers human health. This study aimed to develop an ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for determining sixteen β-agonists in livestock meat. The homogenized samples were subjected to enzymatic hydrolysis using β-glucuronidase/sulfatase at 40 °C for 2 h, extracted with acetonitrile containing 1% acetic acid (v/v), and purified by the one-step Qvet-AG extraction column. The residue was redissolved by 0.1% aqueous formic acid/methanol (9:1, v/v) after blow-drying by nitrogen, and then determined by UHPLC-MS/MS. The results demonstrated that the well linearity was in the range of 0.1–50 μg/L with the correlation coefficient (R2) ≥0.9928, and the limits of detection (LOD) and quantification (LOQ) were 0.01–0.11 μg/kg and 0.04–0.38 μg/kg, respectively. With intraday and interday relative standard deviations (RSDs) being less than 10%, the average recoveries of pork, beef, and lamb at various spiked levels ranged from 62.62–115.93%, 61.35–106.34%, and 62.00–111.83%, respectively. In conclusion, the established method is simple, efficient, sensitive, and suitable for the simultaneous detection of several β-agonist residues in livestock meat.

show abstract

Section: Introductionmentioning

confidence: 99%

Rapid and High-Throughput Determination of Sixteen β-agonists in Livestock Meat Using One-Step Solid-Phase Extraction Coupled with UHPLC-MS/MS

Yan

Ning

Cheng

et al. 2022

Foods

View full text Add to dashboard Cite

show abstract

“…Introduction β-Agonists exhibit short-acting effects and long-acting effects and are frequently used to promote growth in livestock, but problems arise when the residues of such veterinary drugs have the potential to influence human health after being consumed in animal products (1)(2)(3)(4)(5). Evaluation of β-agonist as a veterinary drug is a new concept to implement the principle using medicines for veterinarian by motto: logic and responsibility.…”

Section: ‫تقييم‬ ‫طرح‬ ‫مدة‬ ‫واألغنام‬ ‫الماعز‬ ‫في‬ ‫الراكتوبامين‬ mentioning

confidence: 99%

Assessment of the withdrawal period for ractopamine hydrochloride in the goat and sheep

Lazuardi¹,

Hermanto²,

Restiadi³

2020

IJVS

View full text Add to dashboard Cite

Ractopamine hydrochloride often used as a bronchodilator, but its β-adrenergic agonist effects on un-striated muscle and its withdrawal time have not been assessed for Etawah goats and sheep. The aim of this study was to determine the safe time to slaughter goats and sheep post-treatment with ractopamine. Five clinically healthy adult goats and sheep (20 kg body weight) were treated with a single dose of ractopamine (1 mg, intravenously). Whole blood was sampled from the jugular vein at 120 min, 180 min and 300 min post-treatment. Ractopamine as a veterinary drug was analysed using HPLC at wavelength 225 nm.

show abstract

“…However, the various chromatographic methods reported, such as high-performance liquid chromatography (HPLC) [8,9], gas chromatography-mass spectrometry (GC-MS) [10,11], ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS) [12,13] and capillary electrophoresis (CE) [14,15] are not suitable for field analysis because of the requirement of sophisticated instruments and complicated sample pretreatment. Therefore, plenty of immunoassays based on the enzyme-linked immunosorbent assay (ELISA) [16,17], electrochemistry [18], fluorescence [19], chemiluminescence [20] and surface-enhanced Raman scattering [21] have been prevalent methods recently and are particularly suitable for rapid screening. However, these methods are mostly designed for detection of single or a small amount of analyte due to the complex antibody preparation procedure.…”

Section: Introductionmentioning

confidence: 99%

Expression of Codon Optimized ��2-Adrenergic Receptor in Sf9 Insect Cells for Multianalyte Detection of ��-Agonist Residues in Pork

Liu

Wang

Liu

et al. 2019

Journal of Microbiology and Biotechnology

View full text Add to dashboard Cite

2-AR) was expressed efficiently using Bac-to-Bac Baculovirus Expression System in Sf9 cells as a bio-recognition element for multianalyte screening of β-agonist residues in pork. Sf9 cells were selected as the expression system, and codon optimization of wild-type nucleic acid sequence and time-dependent screening of expression conditions were then carried out for enhancing expression level and biological activity. Under optimum conditions of multiplicity of infection (MOI) = 5 and 48 h post transfection, the protein yield was up to 1.23 mg/ml. After purification by chromatographic techniques, the purified recombinant protein was applied to develop a direct competitive enzyme-linked receptor assay (ELRA) and the efficiency and reliability of the assay was determined. The IC50 values of clenbuterol, salbutamol, and ractopamine were 28.36, 50.70, and 59.57 μg/l, and clenbuterol showed 47.61% and 55.94% cross-reactivities with ractopamine and salbutamol, respectively. The limit of detection (LOD) was 3.2 μg/l and the relevant recoveries in pork samples were in the range of 73.0-91.2%, 69.4-84.6%, and 63.7-80.2%, respectively. The results showed that it had better performance compared with other present nonradioactive receptorbased assays, indicating that the genetically modified β 2-AR would have great application potential in detection of β-agonist residues.

show abstract

Development of a highly sensitive and specific monoclonal antibody based enzyme‐linked immunosorbent assay for the detection of a new β‐agonist, phenylethanolamine A, in food samples

Abstract: The proposed mAb-based ELISA was highly sensitive and specific for PA and could be used as a quantitative/screening method for PA analysis in food samples. © 2016 Society of Chemical Industry.

Cited by 17 publications

References 33 publications

Rapid and High-Throughput Determination of Sixteen β-agonists in Livestock Meat Using One-Step Solid-Phase Extraction Coupled with UHPLC-MS/MS

Rapid and High-Throughput Determination of Sixteen β-agonists in Livestock Meat Using One-Step Solid-Phase Extraction Coupled with UHPLC-MS/MS

Assessment of the withdrawal period for ractopamine hydrochloride in the goat and sheep

Expression of Codon Optimized ��2-Adrenergic Receptor in Sf9 Insect Cells for Multianalyte Detection of ��-Agonist Residues in Pork

Contact Info

Product

Resources

About