Development of a highly permissive cell line from spotted knifejaw (Oplegnathus punctatus) for red sea bream iridovirus

Kawato, Yasuhiko; Yamashita, Hirofumi; Yuasa, Kei; Miwa, Satoshi; Nakajima, Kazuhiro

doi:10.1016/j.aquaculture.2017.02.027

Cited by 27 publications

(20 citation statements)

References 31 publications

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“…The DNA samples derived from the seawater and fish samples were analyzed by real-time PCR targeting the MCP gene of RSIV ( 58 ). To evaluate the recovery rate of KHV as the external standard virus in the seawater sample, another real-time PCR ( 59 ) was performed separately.…”

Section: Methodsmentioning

confidence: 99%

“…Each well contained 20 μl of reaction mixture consisting of 2 μl nucleic acid template and 10 μl of FastStart essential DNA probes master mix (Roche Diagnostics K.K., Tokyo, Japan), with a final concentration of 200 nM for each primer and probe. For the positive control, a serial 10-fold dilution of the plasmid containing the MCP region of RSIV KagYT-96 isolate ( 58 ) was used to draw the standard curve. Real-time PCR assays were performed using a LightCycler 96 Instrument (Roche Diagnostics).…”

Section: Methodsmentioning

confidence: 99%

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Application of Environmental DNA for Monitoring Red Sea Bream Iridovirus at a Fish Farm

Kawato¹,

Mekata²,

Inada³

et al. 2021

Microbiol Spectr

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Application of Environmental DNA for Monitoring Red Sea Bream Iridovirus at a Fish Farm

Kawato¹,

Mekata²,

Inada³

et al. 2021

Microbiol Spectr

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“…Besides, there was also a reduction in fish melanoma cell culture development despite its high applicability as a model for human malignancies. Interestingly, two fish larvae cell lines were developed in the past decade to study fish virology and parasitology (Grunow et al 2011;Kawato et al 2017). A diverse tissue types and fishes were used to develop cell lines mainly to study species specific responses to infections aside other applications discussed in the following sections.…”

Section: Diversity Of Fish Cell Linesmentioning

confidence: 99%

Comprehensive update on inventory of finfish cell lines developed during the last decade (2010–2020)

Thangaraj

Narendrakumar

Geetha

et al. 2021

Reviews in Aquaculture

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Cell culture has become an indispensable part of modern research and is considered the best alternatives for different biological research on whole animals as they mimic the host cellular genetic homogeneity and also reduces the variability of in vivo responses. Hence, there has been increasing development of cell lines by different research groups and companies globally. For this reason, to cover the dearth of knowledge of newly developed fish cell lines, we have made a comprehensive update on new fish cell lines established in the past decade (2010)(2011)(2012)(2013)(2014)(2015)(2016)(2017)(2018)(2019)(2020) to reflect the number of cell lines now available. This review focuses on the appraisal of the literature and of work done in finfish cell culture around the globe during the last decade and summarizes 280 newly established fish cell lines derived from 20 different tissue types of 44 fish families belonging to 21 different countries raising the total finfish cell lines to 783. The highest number of cell lines developed in the past decade was from the fish of the family Cyprinidae, which are most widely cultured in Asian countries. Also, the review discusses the need of new cell line development, different methods of cell line development and further enumerates the various applications and future prospects for these cell lines which we believe will serve as a referral document for researchers in the field.

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“…Due to its taste and high meat quality, spotted knifejaw has great economic potential and emerged as an important aquatic fish species (Shimada, Nokubi, Yamamoto, Murata, & Kumai, ). However, viral infection has become the bottleneck in the spotted knifejaw industry and has greatly limited the annual productivity (Dong et al, ; Kawato, Yamashita, Yuasa, Miwa, & Nakajima, ; Shuang et al, ). Among the viruses that infect spotted knifejaw, the megalocytivirus genus has attracted special scientific interest because of its wide infectious spectrum in fish.…”

Section: Introductionmentioning

confidence: 99%

Cloning of rpl10 in spotted knifejaw ( Oplegnathus punctatus ) and expression pattern analysis after iridovirus infection

Wang

et al. 2019

Aquac Res

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Iridoviridae is an important virus family in aquaculture that is characterized by its wide spectrum of infection in aquatic animals. Recently, spotted knifejaw iridovirus (SKIV) has attracted particular attention because it causes a high death rate and severe economic loss. The ribosomal protein L10 gene (rpl10) is widely reported to participate in the antiviral process in animals and plants. To illustrate its immune roles in spotted knifejaw, we cloned rpl10 and studied its expression patterns in this study. The 3,463 bp rpl10 genomic sequence consisted of 6 exons and 5 introns, with a 1,473 bp transcript encoding a 215 amino acid protein. Tissue expression analysis revealed a wide distribution of rpl10, while higher expression was observed in immune‐related tissues (liver and kidney). Time‐course expression patterns were further examined in three tissues (spleen, liver and kidney) after SKIV infection. Significant upregulation was observed in the spleen and liver at 4 days post infection, while in the kidney, the upregulation occurred at 6 hr post infection. This study would facilitate further investigation into the functionality of rpl10 in the antiviral defence of spotted knifejaw.

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Development of a highly permissive cell line from spotted knifejaw (Oplegnathus punctatus) for red sea bream iridovirus

Cited by 27 publications

References 31 publications

Application of Environmental DNA for Monitoring Red Sea Bream Iridovirus at a Fish Farm

Application of Environmental DNA for Monitoring Red Sea Bream Iridovirus at a Fish Farm

Comprehensive update on inventory of finfish cell lines developed during the last decade (2010–2020)

Cloning of rpl10 in spotted knifejaw ( Oplegnathus punctatus ) and expression pattern analysis after iridovirus infection

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