Comprehensive update on inventory of finfish cell lines developed during the last decade (2010–2020)

Thangaraj, Raja Swaminathan; Narendrakumar, Lekshmi; Geetha, P.; Shanmuganathan, Arun Raja; Dharmaratnam, Arathi; Nithianantham, Sundar Raj

doi:10.1111/raq.12566

Cited by 35 publications

(20 citation statements)

References 243 publications

(248 reference statements)

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“…Cryopreservation at different passage levels followed by the revival after two months of cryo-storage, the DRS cells were successfully recovered with a viability of 70-75% approximately, forming a con uent monolayer of cells after 7 days of plating, which indicates that the cryo-storage is not detrimental to the cells. No changes in the indicators of the functionality of cells, including morphology, attachment, and growth [30], were observed after recovery, which is in agreement with the earlier reports [32,45].…”

Section: Discussionsupporting

confidence: 93%

“…Further, zebra sh skin also possesses a higher regeneration capacity similar to other organs such as ns, heart, and optic nerve [53]. Therefore, cell lines established from the skin tissue could be applicable in regenerative medicine for producing sh skin grafts [45] and the cytotoxicity assessment of silica nanoparticles [9]. Zebra sh skin also offers a relevant biological model to study pigment research [12].…”

Section: Introductionmentioning

confidence: 99%

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Establishment and characterization of a new fibroblast-like cell line from the skin of a vertebrate model, zebrafish (Danio rerio)

et al. 2022

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A novel cell line designated as DRS (Danio rerio skin) was established and characterized from the skin tissue of wild-type zebra sh, Danio rerio, by the explant technique. The cells thrived well in the Leibovitz's -15 medium supplemented with 15% FBS and routinely passaged at regular intervals. The DRS cells mainly feature broblast-like morphology. The culture conditions of the cells were determined by incubating the cells at varying concentrations of FBS and temperature; the optimum was 15% FBS and 28°C, respectively. Cells were cryopreserved and revived with 70-75% viability at different passage levels.Two extracellular products from bacterial species Aeromonas hydrophila and Edwardsiella tarda were tested and found toxic to the DRS cells. Mitochondrial genes, namely COI and 16S rRNA PCR ampli cation and partial sequencing authenticated the species of origin of cells. The modal diploid (2n) chromosome number of the cells was 50. The cells were transfected with pmaxGFP plasmid tested positive for green uorescence at 24-48 h post-transfection, con rming the usefulness of the developed cell line for various in vitro applications.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Establishment and characterization of a new fibroblast-like cell line from the skin of a vertebrate model, zebrafish (Danio rerio)

et al. 2022

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“…Fish cell lines are an extremely valuable tool because of their low cost and reproducibility and are widely used in cell biology, oncology, genomics, genetics, and resource conservation [ 1 , 40 ]. The present study reported the establishment of the C.nasus gonadal somatic cell line and differentiation of medaka SG3 into sperm, which verifies the effect of somatic cells on spermatogonial stem cells.…”

Section: Discussionmentioning

confidence: 99%

Establishment of a Coilia nasus Gonadal Somatic Cell Line Capable of Sperm Induction In Vitro

Kan

Zhang

Jawad

et al. 2022

Biology

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Coilia nasus is an important economic anadromous migratory fish of the Yangtze River in China. In recent years, overfishing and the deterioration of the ecological environment almost led to the extinction of the wild resources of C.nasus. Thus, there is an urgent need to protect this endangered fish. Recently, cell lines derived from fish have proven a promising tool for studying important aspects of aquaculture. In this study, a stable C. nasus gonadal somatic cell line (CnCSC) was established and characterized. After over one year of cell culture (>80 passages), this cell line kept stable growth. RT-PCR results revealed that the CnGSC expressed some somatic cell markers such as clu, fshr, hsd3β, and sox9b instead of germ cell markers like dazl, piwi, and vasa. The strong phagocytic activity of CnGSC suggested that it contained a large number of Sertoli cells. Interestingly, CnGSC could induce medaka spermatogonial cells (SG3) to differentiate into elongated spermatids while co-cultured together. In conclusion, we established a C. nasus gonadal somatic cell line capable of sperm induction in vitro. This research provides scientific evidence for the long-term culture of a gonadal cell line from farmed fish, which would lay the foundation for exploring the regulatory mechanisms between germ cells and somatic cells in fish.

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“…Fibroblasts or other extracellular matrix-secreting cell types could also be incorporated. Fortunately, fibroblast-like cells and cells derived from fin tissue are abundant among fish cell lines (Thangaraj et al 2021 ) and are easily isolated and cultured. Future research into the use of fibroblasts in CS should focus on identifying optimal culture conditions for serum-free co-cultures of fibroblasts with myogenic and adipogenic cells and investigating the effects of these cell types on one another’s proliferation and differentiation.…”

Section: Fish Connective Tissue Vascular Tissue and Skinmentioning

confidence: 99%

Differentiation and Maturation of Muscle and Fat Cells in Cultivated Seafood: Lessons from Developmental Biology

Bomkamp¹,

Musgrove

Marques

et al. 2022

Mar Biotechnol

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Cultivated meat, also known as cultured or cell-based meat, is meat produced directly from cultured animal cells rather than from a whole animal. Cultivated meat and seafood have been proposed as a means of mitigating the substantial harms associated with current production methods, including damage to the environment, antibiotic resistance, food security challenges, poor animal welfare, and—in the case of seafood—overfishing and ecological damage associated with fishing and aquaculture. Because biomedical tissue engineering research, from which cultivated meat draws a great deal of inspiration, has thus far been conducted almost exclusively in mammals, cultivated seafood suffers from a lack of established protocols for producing complex tissues in vitro. At the same time, fish such as the zebrafish Danio rerio have been widely used as model organisms in developmental biology. Therefore, many of the mechanisms and signaling pathways involved in the formation of muscle, fat, and other relevant tissue are relatively well understood for this species. The same processes are understood to a lesser degree in aquatic invertebrates. This review discusses the differentiation and maturation of meat-relevant cell types in aquatic species and makes recommendations for future research aimed at recapitulating these processes to produce cultivated fish and shellfish.

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Comprehensive update on inventory of finfish cell lines developed during the last decade (2010–2020)

Cited by 35 publications

References 243 publications

Establishment and characterization of a new fibroblast-like cell line from the skin of a vertebrate model, zebrafish (Danio rerio)

Establishment and characterization of a new fibroblast-like cell line from the skin of a vertebrate model, zebrafish (Danio rerio)

Establishment of a Coilia nasus Gonadal Somatic Cell Line Capable of Sperm Induction In Vitro

Differentiation and Maturation of Muscle and Fat Cells in Cultivated Seafood: Lessons from Developmental Biology

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