Development of a high sensitivity quantum dot-based fluorescent quenching lateral flow assay for the detection of zearalenone

Chen, Yuan; Fu, Qiangqiang; Xie, Jun; Wang, Hong; Tang, Yong

doi:10.1007/s00216-019-01652-1

Cited by 44 publications

(13 citation statements)

References 30 publications

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“…This is possible because of detection tags that accumulate and give color at T‐ and C‐lines. These tags include colloidal carbon, QDs, fluorescent probes, phosphors, AuNPs, silver NPs, selenium NPs, bioluminescent molecules, enzymes, iridium oxide (IV) NPs, and magnetic particles (Chen, Fu, Xie, Wang, & Tang, 2019; Huang, Aguilar, Xu, Lai, & Xiong, 2016; Quesada‐González et al., 2019; Wang, Xiao, et al., 2019; Zhang, Belwal, et al., 2020; Znoyko, Orlov, Bragina, Nikitin, & Nikitin, 2020). AuNPs (colloidal gold) are mostly used in LFA devices due to (a) ease of production, (b) low cost, (c) optical properties, and (d) higher stability (Morbioli, Mazzu‐Nascimento, Stockton, & Carrilho, 2017).…”

Section: Poc Testing Devicesmentioning

confidence: 99%

Rapid point‐of‐care testing methods/devices for meat species identification: A review

Kumar

Narsaiah

2020

Comp Rev Food Sci Food Safe

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The authentication of animal species is an important issue due to an increasing trend of adulteration and mislabeling of animal species in processed meat products. Polymerase chain reaction is the most sensitive and specific technique for nucleic acid‐based animal species detection. However, it is a time‐consuming technique that requires costly thermocyclers and sophisticated labs. In recent times, there is a need of on‐site detection by point‐of‐care (POC) testing methods and devices under low‐resource settings. These POC devices must be affordable, sensitive, specific, user‐friendly, rapid and robust, equipment free, and delivered to the end users. POC devices should also confirm the concept of micro total analysis system. This review discusses POC testing methods and devices that have been developed for meat species identification. Recent developments in lateral flow assay‐based devices for the identification of animal species in meat products are also reviewed. Advancements in increasing the efficiency of lateral flow detection are also discussed.

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Section: Poc Testing Devicesmentioning

confidence: 99%

Rapid point‐of‐care testing methods/devices for meat species identification: A review

Kumar

Narsaiah

2020

Comp Rev Food Sci Food Safe

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“…The LFIA with fluorescent microparticles have already been used for detection of various microbial pathogens and several inflammation markers [7,8]. Several novel nanoparticles have been generally applied to improve the sensitivity of LFIA, including carbon nanoparticles, quantum dots, fluorescent dyes, magnetic nanolabels and europium nanoparticles (EU-NPS) [9]. EU-NPS as carriers can improve 100-fold sensitivity contrast with colloidal gold nanoparticles labeled in LFIA [10].…”

Section: Introductionmentioning

confidence: 99%

Development of a europium nanoparticles lateral flow immunoassay for NGAL detection in urine and diagnosis of acute kidney injury

et al. 2022

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Background AKI is related to severe adverse outcomes and mortality with Coronavirus Disease 2019 (COVID-19) patients, that early diagnosed and intervened is imperative. Neutrophil gelatinase-associated lipocalin (NGAL) is one of the most promising biomarkers for detection of acute kidney injury (AKI), but current detection methods are inadequacy, so more rapid, convenient and accuracy methods are needed to detect NGAL for early diagnosis of AKI. Herein, we established a rapid, reliable and accuracy lateral flow immunoassay (LFIA) based on europium nanoparticles (EU-NPS) for the detection of NGAL in human urine specimens. Methods A double-antibody sandwich immunofluorescent assay using europium doped nanoparticles was employed and the NGAL monoclonal antibodies (MAbs) conjugate as labels were generated by optimizing electric fusion parameters. Eighty-three urine samples were used to evaluate the clinical application efficiency of this method. Results The quantitative detection range of NGAL in AKI was 1-3000 ng/mL, and the detection sensitization was 0.36 ng/mL. The coefficient of variation (CV) of intra-assay and inter-assay were 2.57-4.98 % and 4.11-7.83 %, respectively. Meanwhile, the correlation coefficient between europium nanoparticles-based lateral fluorescence immunoassays (EU-NPS-LFIA) and ARCHITECT analyzer was significant (R2 = 0.9829, n = 83, p < 0.01). Conclusions Thus, a faster and easier operation quantitative assay of NGAL for AKI has been established, which is very important and meaningful to diagnose the early AKI, suggesting that the assay can provide an early warning of final outcome of disease.

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“…[3,4] In order to reduce the harm of ZEN to human and animal health as well as the economic impact of agriculture and animal husbandry, it is necessary to establish a rapid, accurate, simple, and low-cost detection method for ZEN. [5] At present, thin layer chromatography, [6] high-performance liquid chromatography (HPLC), [7] immunoassay, [8] fluorescence analysis, [9] and electrochemical analysis [10] are frequently used. Among them, the electrochemical method is short in time, low in cost, simple in manufacture, easy to miniaturize, and can be detected on site, but it also has the shortcomings of insufficient stability and sensitivity.…”

Section: Introductionmentioning

confidence: 99%

A homogeneous electrochemical aptasensor based on DNA assembly for zearalenone detection

Xin

et al. 2021

J Chinese Chemical Soc

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In this work, a kind of homogeneous electrochemical aptasensor based on DNA assembly was designed to detect zearalenone (ZEN). The three DNA strands of ZEN aptamer (Apt), complementary strand DNA1, and complementary strand DNA2 are first mixed to form DNA duplex structure, which cannot be cleaved by RecJ f Exonuclease (RecJ f Exo) and hinders the electron transfer after addition to the electrode surface. With the existence of ZEN, the combination of ZEN and Apt destroys the duplex structure, and RecJ f Exo can catalyze the cleavage of Apt to form short DNA strands, which accelerates the electron transfer after addition to the electrode surface. According to the current difference with or without ZEN, the detection purpose is achieved. The linear relationship between I and LgC ZEN was found in the range of 5.0 Â 10 À6 ng/ml to 50 ng/ml, and the detection limit is as low as 0.51 fg/ml. The prepared sensor showed high selectivity, favorable repeatability, and reproducibility and was effectively used for testing the spiked corn flour and beer samples.

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Development of a high sensitivity quantum dot-based fluorescent quenching lateral flow assay for the detection of zearalenone

Cited by 44 publications

References 30 publications

Rapid point‐of‐care testing methods/devices for meat species identification: A review

Rapid point‐of‐care testing methods/devices for meat species identification: A review

Development of a europium nanoparticles lateral flow immunoassay for NGAL detection in urine and diagnosis of acute kidney injury

A homogeneous electrochemical aptasensor based on DNA assembly for zearalenone detection

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