Development of a fluorescence in situ hybridization (FISH) method for rapid detection of Ulva prolifera

Zhang, Qing‐Chun; Liu, Qing; Kang, Zhenjun; Yu, Rencheng; Tian, Yun; Zhou, Meng

doi:10.1016/j.ecss.2014.09.004

Cited by 26 publications

(7 citation statements)

References 37 publications

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“…Both morphological characteristics (Ding and Luan, 2009) and fluorescence in situ hybridization (Zhang et al, 2015) were used for on-site species identification.…”

Section: Species Identification and Biomass Measurementmentioning

confidence: 99%

Cruise observation of Ulva prolifera bloom in the southern Yellow Sea, China

Liu

Wang

et al. 2015

Estuarine, Coastal and Shelf Science

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“…Both morphological characteristics (Ding and Luan, 2009) and fluorescence in situ hybridization (Zhang et al, 2015) were used for on-site species identification.…”

Section: Species Identification and Biomass Measurementmentioning

confidence: 99%

Cruise observation of Ulva prolifera bloom in the southern Yellow Sea, China

Liu

Wang

et al. 2015

Estuarine, Coastal and Shelf Science

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“…Research and monitoring over 10 years have shown that the outbreak mechanisms of U. prolifera green tides can be attributed to multiple factors. Remote sensing data have demonstrated that U. prolifera green tides originally occurred along the Jiangsu coast [ 6–13 ] and gene sequencing data have confirmed that the U. prolifera growing in Porphyra mariculture areas along the Jiangsu coast is the same species as that found in the Yellow Sea [ 14 ]. It also reveals that the unique mud flats for cultivation of Porphyra are favourable habitats for U. prolifera and that more than 20 000 ha of mariculture rafts are good substrata for the propagation of U. prolifera [ 2 , 5 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Ulva prolifera green-tide outbreaks and their environmental impact in the Yellow Sea, China

Zhang

et al. 2019

National Science Review

152

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The Ulva prolifera green tides in the Yellow Sea, China, which have been occurring since 2007, are a serious environmental problem attracting worldwide attention. Despite extensive research, the outbreak mechanisms have not been fully understood. Comprehensive analysis of anthropogenic and natural biotic and abiotic factors reveals that human activities, regional physicochemical conditions and algal physiological characteristics as well as ocean warming and biological interactions (with microorganism or other macroalgae) are closely related to the occurrence of green tides. Dynamics of these factors and their interactions could explain why green tides suddenly occurred in 2007 and decreased abruptly in 2017. Moreover, the consequence of green tides is serious. The decay of macroalgal biomass could result in hypoxia and acidification, possibly induce red tide and even have a long-lasting impact on coastal carbon cycles and the ecosystem. Accordingly, corresponding countermeasures have been proposed in our study for future reference in ecosystem management strategies and sustainable development policy.

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“…Our detection of Ulva species in the gut content samples of U. unicinctus collected at Yantai suggests that spoon worms feed on seaweed detritus. This trophic relationship is easily explained by the green-tide species Ulva frequently blooming in the summer at the Yellow Sea in recent years ( 64 ), resulting in some seaweed detritus drifting to and being buried in the intertidal zones of the coastal line of Yantai and then being consumed. This finding supports the food value of macroalgae to the sediment-dwelling macrofaunal community ( e.g.…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection of Eukaryotic Diets and Gut Mycobiomes in Two Marine Sediment-Dwelling Worms, <i>Sipunculus nudus</i> and <i>Urechis unicinctus</i>

et al. 2018

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The present study aimed to reveal the eukaryotic diets of two economically important marine sediment-inhabiting worms, Sipunculus nudus (peanut worm) and Urechis unicinctus (spoon worm), using clone libraries and phylogenetic analyses of 18S rRNA genes. Fungal rDNA was also targeted and analyzed to reveal mycobiomes. Overall, we detected a wide range of eukaryotic phylotypes associated with the larvae of S. nudus and in the gut contents of both worms. These phylotypes included ciliates, diatoms, dinoflagellates, eustigmatophytes, placidids, oomycetes, fungi, nematodes, flatworms, seaweeds, and higher plants. Oomycetes were associated with the planktonic larvae of S. nudus. The composition of eukaryotic diets shifted greatly across the larval, juvenile, and adult stages of S. nudus, and among different gut sections in U. unicinctus, reflecting lifestyle changes during the ontogeny of the peanut worm and progressive digestion in the spoon worm. Malassezia-like fungi were prevalent in mycobiomes. Epicoccum and Trichosporon-related phylotypes dominated mycobiomes associated with larval individuals and in the gut contents of adults, respectively. The gut mycobiome of S. nudus was successively characterized through the midgut, aspiratory intestines, hindgut, and rectum as having a high proportion of Climacodon-Rhizochaete, Ceriporiopsis, Cladosporium-Pseudomicrostroma, and Malassezia-related species in the libraries. These results emphasize the dynamics of diets and gut mycobiomes in marine benthic animals.

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Development of a fluorescence in situ hybridization (FISH) method for rapid detection of Ulva prolifera

Cited by 26 publications

References 37 publications

Cruise observation of Ulva prolifera bloom in the southern Yellow Sea, China

Cruise observation of Ulva prolifera bloom in the southern Yellow Sea, China

Ulva prolifera green-tide outbreaks and their environmental impact in the Yellow Sea, China

Molecular Detection of Eukaryotic Diets and Gut Mycobiomes in Two Marine Sediment-Dwelling Worms, <i>Sipunculus nudus</i> and <i>Urechis unicinctus</i>

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