Development of a facile and sensitive HPLC‐FLD method via fluorescence labeling for triterpenic acid bioavailability investigation

Abstract

Triterpenic acids are widely distributed in many fruits and are known for their medicinal benefits. The study of bioavailability has been an important task for a better understanding of the triterpenic acids. Although many methods based on fluorescence labeling for triterpenic acid determination have been established, these reported methods needed anhydrous conditions, which are not suitable for the convenient study of triterpenic acid bioavailability. Inspired by that, a versatile method, which overcomes the difficulty of the reported methods, has been first developed in this study. The novel method using 2-[12-benzo[b]acridin-5- (12H)-yl]-acetohydrazide (BAAH) as the fluorescence labeling reagent coupled with high-performance liquid chromatography with fluorescence detection was first developed for the study of triterpenic acid bioavailability. Furthermore, the labeling conditions have been optimized in order to achieve the best fluorescence labeling yield. Under the optimal conditions, the quantitative linear range of analytes was 2-1000 ng mL , and the correlation coefficients were >0.9998. The detection limits for all triterpenic acid derivatives were achieved within the range of 0.28-0.29 ng mL . The proposed method was successfully applied to the study of triterpenic acid bioavailability with excellent applicability and good reproducibility.

Techniques for the analysis of pentacyclic triterpenoids in medicinal plants

“…In some cases, formic acid , ammonium acetate , and acetic acid were added during HPLC analysis to prevent tailing and facilitate the resolution of the components. Furthermore, HPLC technique is usually combined with different detection systems, such as UV detector , diode‐array detector (DAD) , charged aerosol detector (CAD) , fluorescence detector (FLD) , and evaporative light scattering detector (ELSD) . Additionally, HPLC–MS is a more advanced analytical technique that exhibits high sensitivity and selectivity .…”

Determination of Pentacyclic Triterpenoids in Plant Biomass by Porous Graphitic Carbon Liquid Chromatography—Tandem Mass Spectrometry

“…Due to the non-volatility of PCTs, the use of gas chromatography is limited by the need for the derivatization of analytes, which in turn requires additional preliminary purification of the studied extracts [18,19]. In this regard, high-performance liquid chromatography, which allows for the avoidance of tedious and time-consuming sample preparation procedures and the carrying out of direct analyses of plant extracts, can be considered the most promising separation method [20][21][22][23]. Among the detection techniques, atmospheric pressure chemical ionization tandem mass spectrometry (APCI-MS/MS) [18] in the multiple reaction monitoring (MRM) mode demonstrated undoubted advantages, providing the highest selectivity and sensitivity of the analysis [24,25].…”

Simultaneous Determination of Oleanolic Acid and Ursolic Acid by in Vivo Microdialysis via UHPLC-MS/MS Using Magnetic Dispersive Solid Phase Extraction Coupling with Microwave-Assisted Derivatization and Its Application to a Pharmacokinetic Study of Arctiumlappa L. Root Extract in Rats

“…However, OA and UA lack a chromophore and cannot easily gain a charge because of their carboxyl group; thus, they provide a very low sensitivity in relation to UV, fluorescence, and MS detection. Under these circumstances, sensitivity enhancement by chemical derivatization can solve problems. , Some derivatization reagents have been reported for them by HPLC fluorescence detection − and GC-MS . However, there are almost no synthesized derivatization reagents for the enhanced UHPLC-MS/MS determination of OA and UA.…”