Simultaneous Determination of Oleanolic Acid and Ursolic Acid by in Vivo Microdialysis via UHPLC-MS/MS Using Magnetic Dispersive Solid Phase Extraction Coupling with Microwave-Assisted Derivatization and Its Application to a Pharmacokinetic Study of <i>Arctiumlappa</i> L. Root Extract in Rats

Zheng, Zhenjia; Zhu, Shuyun; Dang, Jun; Qiao, Xuguang; Qiu, Zhichang; Tao, Yanduo

doi:10.1021/acs.jafc.7b06015

Cited by 41 publications

(16 citation statements)

References 36 publications

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“…Platanic acid was isolated primarily from Syzygium claviflorum leaves that exhibit anti-HIV activity, with studies of its derivatives suggesting antitumoral activity [30][31][32]. We observed that the G3 sub-network contains a [26]. Both acids are used in Chinese medicine as hepatoprotectives, with oleanolic acid also possessing anti-tumor activity [27,28], and ursolic acid possessing anti-HIV, anti-inflammatory, antiulcer, hypolipidemic, and antiatherosclerotic activities [29].…”

Section: Molecular Networkingmentioning

confidence: 92%

“…The metabolites here are present in all 17 plant extracts, except for triterpenes that are mainly detected in Salacia species. [26]. Both acids are used in Chinese medicine as hepatoprotectives, with oleanolic acid also possessing anti-tumor activity [27,28], and ursolic acid possessing anti-HIV, anti-inflammatory, antiulcer, hypolipidemic, and antiatherosclerotic activities [29].…”

Section: Molecular Networkingmentioning

confidence: 99%

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A Molecular Networking Strategy: High-Throughput Screening and Chemical Analysis of Brazilian Cerrado Plant Extracts against Cancer Cells

Cortelo

Demarque

Dusi

et al. 2021

Cells

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Plants have historically been a rich source of successful anticancer drugs and chemotherapeutic agents, with research indicating that this trend will continue. In this contribution, we performed high-throughput cytotoxicity screening of 702 extracts from 95 plant species, representing 40 families of the Brazilian Cerrado biome. Activity was investigated against the following cancer cell lines: colon (Colo205 and Km12), renal (A498 and U031), liver (HEP3B and SKHEP), and osteosarcoma (MG63 and MG63.3). Dose-response tests were conducted with 44 of the most active extracts, with 22 demonstrating IC50 values ranging from <1.3 to 20 µg/mL. A molecular networking strategy was formulated using the Global Natural Product Social Molecular Networking (GNPS) platform to visualize, analyze, and annotate the compounds present in 17 extracts active against NCI-60 cell lines. Significant cytotoxic activity was found for Salacia crassifolia, Salacia elliptica, Simarouba versicolor, Diospyros hispida, Schinus terebinthifolia, Casearia sylvestris var. lingua, Magonia pubescens, and Rapanea guianensis. Molecular networking resulted in the annotation of 27 compounds. This strategy provided an initial overview of a complex and diverse natural product data set, yielded a large amount of chemical information, identified patterns and known compounds, and assisted in defining priorities for further studies.

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Section: Molecular Networkingmentioning

confidence: 92%

Section: Molecular Networkingmentioning

confidence: 99%

A Molecular Networking Strategy: High-Throughput Screening and Chemical Analysis of Brazilian Cerrado Plant Extracts against Cancer Cells

Cortelo

Demarque

Dusi

et al. 2021

Cells

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“…Currently, studies related to drug–drug interactions mainly concentrate on chemical variations and conversions. Limited attention is given to PK study, which could accurately illustrate the actual biological activity of related ingredients 12 . Thus, a strategy should be developed for the PK study of active ingredients in DZXW and its single herbs simultaneously.…”

Section: Introductionmentioning

confidence: 99%

Comparative pharmacokinetics of Ding‐Zhi‐Xiao‐Wan preparation and its single herbs in rats by using a putative multiple‐reaction monitoring UPLC‐MS/MS method

Sun

Liu

et al. 2020

Phytochemical Analysis

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Introduction The formula of Chinese medicine, Ding‐Zhi‐Xiao‐Wan (DZXW), has the distinct feature of compatibility therapy, which is attributed to the interactions of multi‐herbs. However, the quantification problem caused by the absence of pure reference standards is a bottleneck to clarify the compatibility advantages from the perspective of pharmacokinetics (PKs). Objective This study aimed to develop a putative multiple‐reaction monitor (PMRM) strategy for exploring the comparative PKs of DZXW and its single herbs. Methods First, precursor ion and tandem mass spectrometry (MS/MS) chromatograms were obtained via ultra‐high‐performance liquid chromatography coupled with quadrupole time‐of‐flight MS (UHPLC–Q‐TOF‐MS) under different collision energy (CE) values. Then, the two most abundance ions in the MS/MS chromatograms were chosen as product ions, and CE values were selected according to the abundance of the product ion peaks. Next, a PMRM strategy consisting of optimal MRM parameters was constructed. Finally, the established PMRM parameters were imported to UHPLC coupled with triple quadrupole MS (UHPLC–TQ‐MS) for quantification. Results The strategy was exemplified by the comparative PK study of DZXW and its single herbs. This strategy could extend the PK scopes of multi‐components. The quantitative results displayed substantial variations in PK parameters between DZXW and its single herbs. Conclusion The PK parameters indicated that the DZXW formula could increase the exposure levels of most ingredients and reduce the maximum concentration (Cmax) of Radix Polygala, indicating that herb compatibility could produce synergistic effects and diminish possible toxic effects. This study provides a viable orientation for the compatibility investigation of traditional Chinese medicine.

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“…Among many techniques, solid-phase extraction (SPE) is considered one of the most effective techniques for coextraction due to its convenience, robustness, and rapidity. Therefore, SPE was used here for the analysis of tea samples [14,15].…”

Section: Introductionmentioning

confidence: 99%

Determination of anthraquinone in tea by stable isotope dilution assay-gas chromatography-tandem mass spectrometry

Xie¹,

Huang²,

Deng³

et al. 2020

SN Appl. Sci.

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The aim of this study was to develop an analytical method for the accurate determination of the anthraquinone residue in tea samples (Pu'er tea, oolong tea, green tea, and black tea) by stable isotope dilution assay-gas chromatographytandem mass spectrometry (SIDA-GC-MS/MS). Samples were purified by solid-phase extraction after extraction with n-hexane:acetone (1:1, v/v). The anthraquinone residue was then detected by selected reaction monitoring (SRM) in electron ionization mode. Anthraquinone-d8 was added in the tea sample extraction process to eliminate the matrix effect. The average recoveries were in the range of 84.2-98.1% at different spiked levels (0.02, 0.04 and 0.08 mg kg −1 ), and the relative standard deviations were below 9.7%. The limits of quantification, calculated as 10 times the standard deviation, was 0.02 mg kg −1 . The developed method is simple, rapid, and accurate to quantitate the concentration of anthraquinone residues in tea samples.

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Simultaneous Determination of Oleanolic Acid and Ursolic Acid by in Vivo Microdialysis via UHPLC-MS/MS Using Magnetic Dispersive Solid Phase Extraction Coupling with Microwave-Assisted Derivatization and Its Application to a Pharmacokinetic Study of Arctiumlappa L. Root Extract in Rats

Cited by 41 publications

References 36 publications

A Molecular Networking Strategy: High-Throughput Screening and Chemical Analysis of Brazilian Cerrado Plant Extracts against Cancer Cells

A Molecular Networking Strategy: High-Throughput Screening and Chemical Analysis of Brazilian Cerrado Plant Extracts against Cancer Cells

Comparative pharmacokinetics of Ding‐Zhi‐Xiao‐Wan preparation and its single herbs in rats by using a putative multiple‐reaction monitoring UPLC‐MS/MS method

Determination of anthraquinone in tea by stable isotope dilution assay-gas chromatography-tandem mass spectrometry

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