Development of a dural substitute from synthetic bioabsorbable polymers

Yamada, Keisuke; Miyamoto, Susumu; Nagata, Izumi; Kikuchi, Hiroe; Ikada, Yoshito; Iwata, Hiroo; Yamamoto, Kazuo

doi:10.3171/jns.1997.86.6.1012

Cited by 109 publications

(65 citation statements)

References 28 publications

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“…More research is needed to optimize the intended in vivo delivery construct. Others have previously shown that PCL is an excellent synthetic dural substitute showing no infection or significant adhesion to the underlying cortex [34]. Therefore, delivery of transplanted DSCs on a dural substitute material may encourage host DSCs to migrate to the wound bed to participate in tissue repair.…”

Section: Introductionmentioning

confidence: 97%

Osteogenic differentiation of dura mater stem cells cultured in vitro on three-dimensional porous scaffolds of poly(ε-caprolactone) fabricated via co-extrusion and gas foaming

et al. 2008

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A novel scaffold fabrication method utilizing both polymer blend extrusion and gas foaming techniques to control pore size distribution is presented. Seventy-five per cent of all pores produced using polymer blend extrusion alone were less than 50microm. Introducing a gas technique provided better control of pore size distribution, expanding the range from 0-50 to 0-350microm. Varying sintering time, annealing temperature and foaming pressure also helped to reduce the percentage of pore sizes below 50microm. Scaffolds chosen for in vitro cellular studies had a pore size distribution of 0-300microm, average pore size 66+/-17microm, 0.54+/-0.02% porosity and 98% interconnectivity, measured by micro-computed tomography (microCT) analysis. The ability of the scaffolds to support osteogenic differentiation for subsequent cranial defect repair was evaluated by static and dynamic (0.035+/-0.006ms(-1) terminal velocity) cultivation with dura mater stem cells (DSCs). In vitro studies showed minimal increases in proliferation over 28 days in culture in osteogenic media. Alkaline phosphatase expression remained constant throughout the study. Moderate increases in matrix deposition, as assessed by histochemical staining and microCT analysis, occurred at later time points, days 21 and 28. Although constructs cultured dynamically showed greater mineralization than static conditions, these trends were not significant. It remains unclear whether bioreactor culture of DSCs is advantageous for bone tissue engineering applications. However, these studies show that polycaprolactone (PCL) scaffolds alone, without the addition of other co-polymers or ceramics, support long-term attachment and mineralization of DSCs throughout the entire porous scaffold.

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Section: Introductionmentioning

confidence: 97%

Osteogenic differentiation of dura mater stem cells cultured in vitro on three-dimensional porous scaffolds of poly(ε-caprolactone) fabricated via co-extrusion and gas foaming

et al. 2008

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“…Dural substitutes could be placed as an onlay over dural defects or sutured [3]. Autologous dura grafts such as pericranium, temporalis fascia and fascia lata could be harvested to complete the dural closure, but several drawbacks were encountered for example insufficient quantity, unsuitable quality, morbidity and pain [4,5]. Allograft based on lyophilized homologous dura mater could be employed, but it was associated with the development of atrophy or adhesion with the brain & Warinkarn Hemstapat kamon_ann@hotmail.com; warinkarn.hem@mahidol.ac.th surface and suffered from the risk of latent virus infections and Creutzfeldt-Jacob disease (CJD) [6,7].…”

Section: Introductionmentioning

confidence: 99%

Bilayer oxidized regenerated cellulose/poly ε-caprolactone knitted fabric-reinforced composite for use as an artificial dural substitute

Suwanprateeb

Luangwattanawilai

Theeranattapong

et al. 2016

J Mater Sci: Mater Med

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A novel bilayer knitted fabric-reinforced composite for potentially being used as a dural substitute was developed by solution infiltration of oxidized regenerated cellulose knitted fabric (ORC) with poly ε-caprolactone (PCL) solution at various concentrations ranging 10-40 g/100 mL. It was found that the density of all formulations did not differ significantly and was lower than that of the human dura. Microstructure of the samples typically comprised a bilayer structure having a nonporous PCL layer on one side and the ORC/PCL composite layer on another side. Tensile modulus and strength of the samples initially decreased with increasing PCL solution concentration for up to 20 g/100 mL and re-increased again with further increasing PCL solution concentration. Strain at break of all formulations were not significantly different. Watertight test revealed that all composites could prevent leakage at the pressure within the normal range of intracranial pressure. In vitro degradation study revealed that the weight loss percentage and change in tensile properties of all samples displayed biphasic profile comprising an initially rapid decrease and followed by a gradual decrease with incubation times afterward. Micro and macro porous channels were observed to be in situ generated in the composite layer by ORC dissolution and PCL resorption during degradation while nonporous layer remained relatively unchanged. The degradation rate was found to decrease with increasing PCL solution concentration. In vitro biocompatibility using alamar blue assay on selected samples showed that fibroblasts could attach and proliferate well at all incubation periods.

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“…Moreover, in watertight closure holes created by suturing graft to dura mater could cause a cerebrospinal fluid leakage. The strength of synthetic absorbable grafts is only guaranteed for the first 2 weeks: these materials are often brittle and they tends to give cerebrospinal fluid leaks, without preserving the guest from serious inflammatory reactions (Yamada et al, 1997). Other drawbacks of polytetrafluoroethylene are represented by its strong surface tension and its lack of adaptability, frequent appearance of friction injury with underlying brain and meninges, which may cause bleeding and inflammation (Islam, 2004, as cited in Yamagata, 1993.…”

Section: Synthetic Graftsmentioning

confidence: 99%

“…Others have developed a composite sheet composed of two layers of L-lactic acid -caprolactone with polyglycolic acid nonwoven fabric sandwiched between the layers. Clinical trials of this material are pending (Yamada et al, 1997). The vicryl mesh as a suitable dural substitute, with potential advantages, was performed to avoid cerebrospinal fluid leak, following posterior fossa surgery (Verheggen et al, 1997).…”

Section: Future Trendsmentioning

confidence: 99%