Development of a cryopreservation protocol for testicular interstitial cells with the account of temperature intervals for controlled cooling below −60°С

Gurina, T. M.; Pakhomov, Oleksandr; Kyryliuk, A.L.; Божок, Г. А.

doi:10.1016/j.cryobiol.2011.01.011

Cited by 10 publications

(10 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present study used a combination of DMSO and EG in both vitrification protocols with varying concentrations, finding that lower concentrations of cryoprotectants (15% of DMSO, 15% of EG) were more efficient in preserving cell viability. This result obtained on cell viability corroborates with the best protocol tested by Gurina et al . (2011) - slow cryopreservation of testicular tissue from adult Wistar rats using DMSO as cryoprotectant at a rate of 67.7% viability.…”

Section: Discussionsupporting

confidence: 91%

Wistar rats immature testicular tissue vitrification and heterotopic grafting

Benvenutti

Salvador

Til

et al. 2018

JBRA Assisted Reproduction

View full text Add to dashboard Cite

ObjectiveTo evaluate the efficiency of two vitrification protocols for rat immature testicular tissue and heterotopic transplantation.MethodsTwenty-four pre-pubertal Wistar rats were divided into three groups (n=8). After orchiectomy, testicular fragments (3mm) from Groups 1 and 2 were vitrified with different cryoprotectant concentration solutions, using sterile inoculation loops as support. After warming up, the fragments were submitted to cell viability assessment by Trypan blue and histological evaluation. Vitrified (Groups 1 and 2) and fresh (Group 3) fragments were grafted to the animals periauricular region. After 8 weeks of grafting, the implant site was histologically analyzed.ResultsThe viability recovery rate from Group 1 (72.09%) was higher (p=0.02) than that from Group 2 (59.19%). Histological analysis showed similar tubular integrity between fresh fragments from Groups 1 and 3. Group 2 samples presented lower tubular integrity. We ran histological analyses in the grafts from the Groups. In all groups, it was possible to see the implant site, however, no fragment of testicular tissue or signs of inflammation were histologically found in most samples from Groups 1 and 3. In one sample from Group 2, we found degenerated seminiferous tubules with necrosis and signs of an inflammatory process. In another sample from Group 2, we found seminiferous tubules in the implant site.ConclusionThe vitrification of pre-pubertal testicular tissue of rats showed little damage to cell viability through histological analysis when we used cryoprotectants in a lower concentration. Heterotopic transplantation could not preserve the structural organization of the testicular tissue.

show abstract

Section: Discussionsupporting

confidence: 91%

Wistar rats immature testicular tissue vitrification and heterotopic grafting

Benvenutti

Salvador

Til

et al. 2018

JBRA Assisted Reproduction

View full text Add to dashboard Cite

show abstract

“…When the temperature -70 °C was reached the samples were plunged into liquid nitrogen (-196 °C). The method was developed in the Institute for problems of cryobiology and cryomedicine, NAS of Ukraine, and used CM-1 [7][8][9]. The method was compared with method 3.…”

Section: Methodsmentioning

confidence: 99%

“…The cited researches also use the cooling rate 1 °C/min. We have worked out and described an alternative method for cryopreservation of ICs that promotes the survival of Leydig cells in IC suspension [7][8][9]. These two methods are common in a way they both use DMSO and blood serum but they differ in the cooling rate in the bulk ice mass melting and the eutectic melting ranges of cryopreservation media that promotes higher survival of Leydig cells in samples.…”

Section: Introductionmentioning

confidence: 99%

The impact of cryopreservation with polyvinyl alcohol on the survival and functional activity of rat testis interstitial cells

Pakhomov

Sidorenko

2021

Biopolym. Cell

View full text Add to dashboard Cite

“…); (iii) 10 ng/mL hCG + 10 À8 M T-2 toxin; (iv) 10 ng/mL hCG + 10 À9 M T-2 toxin. The Cell viability was determined by the trypan blue dye-exclusion test, according to Gurina in 2011(Gurina et al, 2011. The viability of the control and treated cells was over 90%.…”

Section: Cell Treatmentmentioning

confidence: 99%