Development of a Cross-Linked Polysaccharide of<i>Ligusticum wallichii</i>– Squid Skin Collagen Scaffold Fabrication and Property Studies for Tissue-Engineering Applications

Zhang, Weijie; Wang, Peng; Wang, Yonggang; Fu, Wei; Pu, Ximing; Zhang, Fei; Hua, Dawei; Ma, Shuhua; Chen, Zhuo; Wang, Minggang

doi:10.1080/00914037.2013.769249

Cited by 11 publications

(4 citation statements)

References 48 publications

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“…One of the key factors of tissue engineering is to create a three-dimensional scaffold with suitable properties also, such as degradation rate, high porosity, and interconnected pores. Typically, biodegradable polymeric scaffolds are fabricated using different methods [14,15]. In natural tissues, cells are surrounded by extracellular matrix, which has physical structural features ranging from nanometer to micrometer scale.…”

Section: Introductionmentioning

confidence: 99%

Cellular Response of Limbal Stem Cells on PHBV/Gelatin Nanofibrous Scaffold for Ocular Epithelial Regeneration

Baradaran‐Rafii

Biazar

Keshel

2015

International Journal of Polymeric Materials and Polymeric Biom

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The aim of this study was to develop a blend of nanofibrous poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)=gelatin substrate for limbal stem cell (LSC) expansion that can serve as a potential alternative substrate to replace human amniotic membrane. The human Limbus stem cell was used to evaluate the biocompatibility of substrates (nanofibrous scaffold, and human amniotic membrane) based on their phenotypic profile, viability, proliferation, and attachment ability. Biocompatibility results indicated that the all substrates were highly biocompatible, as LSCs could favorably attach and proliferate on the nanofibrous surface. Microscopic figures showed that the human LSCs were firmly anchored to the substrates and were able to retain a normal corneal stem cell phenotype. Microscopic analyses illustrated that cells infiltrated the nanofibers and successfully formed a three-dimensional corneal epithelium, which was viable for two weeks. Immunocytochemistry (ICC) and real time-PCR results revealed no change in the expression profile of LSCs grown on nanofibrous substrate when compared to those grown on human amniotic membrane. In addition, electrospun nanofibrous PHBV substrate provides not only a milieu supporting LSCs expansion, but also serves as a useful alternative carrier for ocular surface tissue engineering and could be used as an alternative substrate to amniotic membrane.

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Section: Introductionmentioning

confidence: 99%

Cellular Response of Limbal Stem Cells on PHBV/Gelatin Nanofibrous Scaffold for Ocular Epithelial Regeneration

Baradaran‐Rafii

Biazar

Keshel

2015

International Journal of Polymeric Materials and Polymeric Biom

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“…Creation of a three-dimensional scaffold which has suitable properties such as high porosity, interconnected pores, and high degradation rate is one of the key factors of tissue engineering. Biodegradable polymeric scaffolds can typically be fabricated by different methods [3,4]. In natural tissues, extracellular matrixes which have physical structures ranging from nanometer to micrometer scale.…”

Section: Introductionmentioning

confidence: 99%

Design of Curcumin-Loaded Electrospun Polyhydroxybutyrate Mat as a Wound Healing Material

L¹,

Biazar²,

Taleghani³

et al. 2020

Nano BioMed ENG

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Nanotechnology and tissue engineering have accelerated wound healing. Polyhydroxyalkanoates, with suitable physical, biological and mechanical properties, can be considered as a good candidate in tissue repair and regeneration. In this study, nanofibrous mats of polyhydroxybutyrate (PHB) containing curcumin as a wound healing agent, were designed by electrospinning method. The samples were evaluated by microscopic and mechanical analyses, cell assays and microbial tests. The results of microscopic images showed that the diameter of fibers increased with the increase in the curcumin concentration. The elongation and elasticity modulus of nanofibers increased and decreased respectively, with the increase in the amount of curcumin. Drug release study indicated that increasing the curcumin concentration into nanofibers accelerated rate of drug release. Cytotoxicity results of nanofibrous samples with lower curcumin showed better biocompatibility. The strongest antibacterial activity was shown by the sample with 3% curcumin. In addition, Curcumin-loaded nanofibrous PHB can be potential candidates for wound healing.

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“…So far, various materials and scaffolds have been tested for the transportation of stem cells. To treat severe ocular surface damage and a deficiency in limbal stem cells (LSCs), which are irreplaceable for corneal healing, various carriers for the culturing of LSCs and for their transplantation onto the recipient eye have been tested (Biazar 2013, Hartgerink et al 2002, Rama et al 2001, Schwab et al 2006, Tsai et al 2000, Zhang et al 2013. In natural tissues, cells are surrounded by extracellular matrix (ECM), which has physical structural features ranging from nanometer to micrometer scale.…”

Section: Introductionmentioning

confidence: 99%

Electrospun mat with eyelid fat-derived stem cells as a scaffold for ocular epithelial regeneration

Momenzadeh

Baradaran‐Rafii

Keshel

et al. 2016

Artificial Cells, Nanomedicine, and Biotechnology

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The aim of this study was to develop nanofibrous gelatin substrates for eyelid fat stem cell (EFSC) expansion that can serve as a potential alternative substrate to replace human amniotic membrane. Biocompatibility results indicated that all substrates were highly biocompatible, as EFSCs could favorably attach and proliferate on the nanofibrous surfaces. Microscopic figures showed that the EFSC were firmly anchored to the substrates and were able to retain a normal stem cell phenotype. Immunocytochemistry (ICC) and real time-PCR results revealed change in the expression profile of EFSCs grown on nanofibrous substrates when compared to those grown on control in epithelial induction condition. In addition, electrospun gelatin mats especially oriented scaffold provides not only a milieu supporting EFSCs expansion, but also serves as a useful alternative carrier for ocular surface tissue engineering and could be used as an alternative substrate to amniotic membrane.

show abstract

Development of a Cross-Linked Polysaccharide ofLigusticum wallichii– Squid Skin Collagen Scaffold Fabrication and Property Studies for Tissue-Engineering Applications

Cited by 11 publications

References 48 publications

Cellular Response of Limbal Stem Cells on PHBV/Gelatin Nanofibrous Scaffold for Ocular Epithelial Regeneration

Cellular Response of Limbal Stem Cells on PHBV/Gelatin Nanofibrous Scaffold for Ocular Epithelial Regeneration

Design of Curcumin-Loaded Electrospun Polyhydroxybutyrate Mat as a Wound Healing Material

Electrospun mat with eyelid fat-derived stem cells as a scaffold for ocular epithelial regeneration

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