Development of a codominant CAPS marker for allelic selection between canary yellow and red watermelon based on SNP in lycopene β-cyclase (LCYB) gene

Bang, Hyochoong; Kim, Sunggil; Leskovar, Daniel I.; King, Stephen R.

doi:10.1007/s11032-006-9076-4

Cited by 112 publications

(97 citation statements)

References 16 publications

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“…In different maize genotypes, polymorphisms capable of increasing the proportion of provitamin A carotenoids have been identified in the genes for lycopene «-cyclase (Harjes et al, 2008) and b-carotene hydroxylase 1 . A single SNP leading to an amino acid replacement in a conserved position of LCY-b, and probably leading to impaired catalytic function, was found to define red-and yellow-fleshed watermelon (Citrullis vulgaris) due to the accumulation of lycopene and cyclic carotenoids, respectively (Bang et al, 2007). A single amino acid substitution in the enzyme LCY-e is responsible for the introduction of one or two ionone rings (Cunningham and Gantt, 2001).…”

Section: Discussionmentioning

confidence: 99%

Provitamin A Accumulation in Cassava (Manihot esculenta) Roots Driven by a Single Nucleotide Polymorphism in a Phytoene Synthase Gene

et al. 2010

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Cassava (Manihot esculenta) is an important staple crop, especially in the arid tropics. Because roots of commercial cassava cultivars contain a limited amount of provitamin A carotenoids, both conventional breeding and genetic modification are being applied to increase their production and accumulation to fight vitamin A deficiency disorders. We show here that an allelic polymorphism in one of the two expressed phytoene synthase (PSY) genes is capable of enhancing the flux of carbon through carotenogenesis, thus leading to the accumulation of colored provitamin A carotenoids in storage roots. A single nucleotide polymorphism present only in yellow-rooted cultivars cosegregates with colored roots in a breeding pedigree. The resulting amino acid exchange in a highly conserved region of PSY provides increased catalytic activity in vitro and is able to increase carotenoid production in recombinant yeast and Escherichia coli cells. Consequently, cassava plants overexpressing a PSY transgene produce yellow-fleshed, high-carotenoid roots. This newly characterized PSY allele provides means to improve cassava provitamin A content in cassava roots through both breeding and genetic modification.

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Section: Discussionmentioning

confidence: 99%

Provitamin A Accumulation in Cassava (Manihot esculenta) Roots Driven by a Single Nucleotide Polymorphism in a Phytoene Synthase Gene

et al. 2010

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“…The different alleles identified in the Bob-LCY1 gene of B. orellana associated with fruit and flower colour and bixin contents might also be involved in the regulation of carotenoid synthesis, as proposed for watermelon, papaya, chilli and maize (Bang et al 2007;Ha et al 2007;Harjes et al 2008;Devitt et al 2010). The identification of the genes involved in the bixin pathway is necessary to completely understand the regulation of this gene and the molecular basis for the heterosis phenotype associated with bixin production.…”

Section: Discussionmentioning

confidence: 90%

“…We therefore investigated allelic variation in the Bob-LCY1 gene using the SSCP method (Orita et al 1989), a technique that detects DNA variation in small amplicons, to identify SNPs associated with phenotypic traits. This approach has been successfully used to detect molecular markers in papaya, maize and watermelon (Devitt et al 2010;Harjes et al 2008;Bang et al 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Bang et al (2007) characterized the b-LCY gene in two varieties of watermelon with contrasting fruit types and found that a SNPs resulting in a Phe to Val change at position 226 of the b-LCY protein was associated with the red to yellow colour change in the fruit. A similar case in papaya involved fruit carotene content (Devitt et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Thus, in two accessions of achiote differing in pigment content, Bob-LCY1 mRNA expression was correlated with carotene accumulation (Rodríguez-Á vila et al 2011). Additionally, in some plants, such as capsicum, watermelon, papaya and maize, DNA sequence polymorphisms in cyclase genes are associated with the pigment phenotype (Bang et al 2007;Ha et al 2007;Harjes et al 2008;Devitt et al 2010). Based on these observations, the aim of the present study was to investigate the existence of polymorphisms in the Bob-LCY1 gene in different achiote accessions and test a potential correlation to bixin levels.…”

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confidence: 96%

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Functional polymorphism in lycopene beta-cyclase gene as a molecular marker to predict bixin production in Bixa orellana L. (achiote)

et al. 2016

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Bixin is an apocarotenoid obtained from the seed aril of Bixa orellana L., a tropical plant known as achiote in Mexico. This compound is the second most commonly used natural colouring for food and pharmaceutical industries. B. orellana is an outcrossing species that displays high genetic variability. Recently, the colour traits of sexual organs were associated with the biosynthesis and accumulation of bixin in mature seeds. Herein, we describe a new approach for genotype-phenotype association by surveying lycopene beta-cyclase (Bob-LCY1) gene variation in sixteen achiote accessions divided into three groups according to contrasting traits, such as flower colour, fruit colour and bixin production. Using a combination of single-strand conformational polymorphism techniques and the sequencing of polymorphic bands, we identified several single-nucleotide polymorphisms that divided the accessions into three haplotypes. Surprisingly, we observed that these three haplotypes were consistent with the same three groups previously characterized by phenotypic traits. We derived a putative sequence for the Bob-LCY1 gene and surveyed the variations in this sequence. The heterozygosity of Bob-LCY1 alleles resulted in a higher bixin content, likely associated with heterosis for this metabolite. These findings augment the Electronic supplementary material The online version of this article (doi:10.1007/s11032-016-0555-y) contains supplementary material, which is available to authorized users.

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Plant Breeding for Human Nutritional Quality

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Pollak

Clevidence

et al. 2009

Plant Breeding Reviews

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Development of a codominant CAPS marker for allelic selection between canary yellow and red watermelon based on SNP in lycopene β-cyclase (LCYB) gene

Cited by 112 publications

References 16 publications

Provitamin A Accumulation in Cassava (Manihot esculenta) Roots Driven by a Single Nucleotide Polymorphism in a Phytoene Synthase Gene

Provitamin A Accumulation in Cassava (Manihot esculenta) Roots Driven by a Single Nucleotide Polymorphism in a Phytoene Synthase Gene

Functional polymorphism in lycopene beta-cyclase gene as a molecular marker to predict bixin production in Bixa orellana L. (achiote)

Plant Breeding for Human Nutritional Quality

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