Provitamin A Accumulation in Cassava (<i>Manihot esculenta</i>) Roots Driven by a Single Nucleotide Polymorphism in a Phytoene Synthase Gene

Welsch, Ralf; Arango, Jacobo; Bär, Cornelia; Salazar, Bertha; Al‐Babili, Salim; Beltrán, Jesús; Chavarriaga, Paul; Ceballos, Hernán; Tohmé, Joe; Beyer, Peter

doi:10.1105/tpc.110.077560

Cited by 256 publications

(224 citation statements)

References 38 publications

Supporting

Mentioning

212

Contrasting

Unclassified

Order By: Relevance

“…The beta carotene content of C. annuum jaune was lower than those of the other two varieties studied (Table 3). Marin et al [31] reported a high pro-vitamin A content in the mature stage of peppers. Recently, Guzman et al [11] indicated that orange or yellow color in Capsicum fruit can have very low amounts of the β-carotene level.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, Guzman et al [11] indicated that orange or yellow color in Capsicum fruit can have very low amounts of the β-carotene level. The level of β-carotene in pepper depends upon various factors: the level of expression of the genes governing carotenogenesis, physiological and morphological characteristics intrinsic to the cultivar, and probably growth conditions [31].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Bioactive compounds and some vitamins from varieties of pepper (Capsicum) grown in CÔTE D’IVOIRE

Kouassi¹

2012

PAB

View full text Add to dashboard Cite

The aim of this work was to isolate groups of bioactive compounds of biological interest from some varieties of pepper (Capsicum) grown and consumed in Côte d'Ivoire, to assess the ability of organic solvent to extract these compounds and to determine ascorbic acid and beta-carotene content. Polar, non polar and acid compounds of intermediate polarity obtained by thin layer chromatography (TLC) were used to isolate from the acetone extracts, from 33 to 34 compounds for C. annuum and from 27 to 33 for C. frutescens. The number of lipid compounds isolated from C. annuum varies from 32 to 33 and 24 to 29 in C. frutescens. The total non-lipid compounds obtained by the dichloromethane, acetone and methanol extract ranges from 88 to 103 for C. annuum, and from 84 to 96 for C. frutescens. The number of lipid compounds was higher for C. annuum than for C. frutescens. The lipid compounds and non-lipid compounds were highly variable depending on the variety of Capsicum. The ascorbic acid and beta carotene contents were obtained by High-performance liquid chromatography (HPLC). Beta-carotene content in pepper studied ranged from 68 to 535 µg 100 g -1 FW and ascorbic acid from 86 to 96 mg 100 g -1 FW. The current study may contribute to the knowledge of the nutritional and the bioactive compounds of the fruit and may offer knowledge of the potential health benefits for bell pepper consumers in Côte d'Ivoire..

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Bioactive compounds and some vitamins from varieties of pepper (Capsicum) grown in CÔTE D’IVOIRE

Kouassi¹

2012

PAB

View full text Add to dashboard Cite

show abstract

“…Yellow-colored petals of chrysanthemums accumulate carotenoids, while whitecolored petals lack them because of the presence of carotenoid cleavage dioxygenase (CCD), which degrades carotenoids (Ohmiya et al 2006). Cassava (Manihot esculenta Crányz) cultivars accumulate carotenoids in their yellow tubers (Welsch et al 2010). The expression level of genes encoding carotenoid biosynthetic enzymes did not differ from other cultivars.…”

Section: Discussionmentioning

confidence: 99%

Biochemical and molecular characterization of orange- and tangerine-colored rice calli

Ishihara

Ohishi

Yamada

et al. 2015

Plant Biotechnology

View full text Add to dashboard Cite

We established an orange (L) and a tangerine (D) colored callus line by repeatedly subculturing rice callus line (N). The L line accumulated lycopene, β-carotene, and two lycopene isomers, while the D line accumulated much higher concentration of lycopene as well as a lycopene isomer than the L line. Genomic sequencing revealed the presence of SNP that results in the incomplete lycopene β-cyclase (β LCY) enzyme in the D line. We analyzed expression levels of carotenoid biosynthetic genes by qRT-PCR. Regarding the 2-C-methyl-D-erythritol-4-phosphate pathway genes, expression of the gene encoding geranylgeranyl diphosphate synthase 1 (GGPS1) was enhanced in both L and D lines as compared to the N line. Regarding the carotenoid pathway genes, the transcript amounts of the genes encoding phytoene synthase 2, phytoene desaturase, and ζ-carotene desaturase, were larger in the L line than in the N line; however, this pattern was not found in the D line. The expression levels of β LCY and lycopene ε-cyclase (ε LCY) gene in the D line and ε LCY in the L line were lower than in the N line. The transcript amounts of two carotenoid oxygenase genes were decreased both in L and D lines. We concluded that increased expression of GGPS1 and reduced expression of ε LCY, β LCY, and carotenoids oxygenase genes might allow an excess accumulation of carotenoids in L and D lines, and that the mutation in β LCY is responsible for the conspicuous phenotype in the D line as compared to the L line.

show abstract

“…The overexpression of DXP synthase in tomato is an example of the first strategy, producing a carotenoid precursor that increases flux in the entire pathway and enhances the total carotenoid content (Enfissi et al 2005). Cassava roots expressing the bacterial CrtB gene accumulated up to 21 lg/g of carotenoids, a 34-fold increase with respect to the wild type (Welsch et al 2010). The replacement of the daffodil gene with its maize ortholog is the basis of Golden Rice 2, which produces up to 37 lg of carotenoids per gram dry weight (DW) of grain, of which 31 lg/g is b-carotene (Paine et al 2005).…”

Section: Vitamin Amentioning

confidence: 99%

The contribution of transgenic plants to better health through improved nutrition: opportunities and constraints

et al. 2012

View full text Add to dashboard Cite

Malnutrition is a prevalent and entrenched global socioeconomic challenge that reflects the combined impact of poverty, poor access to food, inefficient food distribution infrastructure, and an over-reliance on subsistence mono-agriculture. The dependence on staple cereals lacking many essential nutrients means that malnutrition is endemic in developing countries. Most individuals lack diverse diets and are therefore exposed to nutrient deficiencies. Plant biotechnology could play a major role in combating malnutrition through the engineering of nutritionally enhanced crops. In this article, we discuss different approaches that can enhance the nutritional content of staple crops by genetic engineering (GE) as well as the functionality and safety assessments required before nutritionally enhanced GE crops can be deployed in the field. We also consider major constraints that hinder the adoption of GE technology at different levels and suggest policies that could be adopted to accelerate the deployment of nutritionally enhanced GE crops within a multicomponent strategy to combat malnutrition.

show abstract

Provitamin A Accumulation in Cassava (Manihot esculenta) Roots Driven by a Single Nucleotide Polymorphism in a Phytoene Synthase Gene

Cited by 256 publications

References 38 publications

Bioactive compounds and some vitamins from varieties of pepper (Capsicum) grown in CÔTE D’IVOIRE

Bioactive compounds and some vitamins from varieties of pepper (Capsicum) grown in CÔTE D’IVOIRE

Biochemical and molecular characterization of orange- and tangerine-colored rice calli

The contribution of transgenic plants to better health through improved nutrition: opportunities and constraints

Contact Info

Product

Resources

About