Development in culture of the chick embryo from cleavage to hatch

Naito, Mitsuru; Perry, Margaret M.

doi:10.1080/00071668908417145

Cited by 39 publications

(12 citation statements)

References 13 publications

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“…For the fitness of development of donor embryos, WL eggshells which is larger than those of donor embryos (KO) were used as a recipient for the surrogate system (Naito and Perry, 1989). Initially, KO embryos at 3-day and 4-day-old (HH stage 20∼24) (Hamburger and Hamilton, 1951) were transferred into WL surrogate eggshells and incubated until hatching of chicks.…”

Section: Resultsmentioning

confidence: 99%

Normal Development and Hatchability of Korean Oge Chickens in White Leghorn Surrogate Eggshells

Kang¹,

Lee²,

Lee³

et al. 2014

Korean Journal of Poultry Science

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The avian embryos have been used as a good model to study embryonic development. Due to its unique development in the eggshell, avian embryos can be cultured and hatch in the surrogate eggshell system. In this study, we examined the viability, normal development and hatchability of Korean Oge (KO) chicken embryos in White Leghorn (WL) surrogate eggshells. Donor KO embryos at 3-day and 4-day-old were transferred into recipient WL eggshells, incubated for further 18 days at 37.5℃ with 70% of humidity until hatching. The viability of 3-day-old KO embryos at 7, 14 and 21 day in surrogate eggshell were 70.0%, 43.8% and 23.1%, respectively. In contrast, the viability of 4-day-old KO embryos at 7, 14 and 21 day in surrogate eggshells were 87.1%, 55.6% and 36.0%, respectively. The hatchability of KO embryos transferred into surrogate eggshells at 3-day-old was 23.1%, whereas embryos transferred at 4-day-old was 36.0%. Furthermore, the development of all viable embryos from 3-day group and 4-day group were normal. Our results suggested that culture of KO embryos in WL surrogate eggshells is highly possible, and transfer of donor embryos at 4-day-old may yield higher percentage of hatchability. This study may provide potential knowledge for the conservation of wild and endangered birds through surrogate system.

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Section: Resultsmentioning

confidence: 99%

Normal Development and Hatchability of Korean Oge Chickens in White Leghorn Surrogate Eggshells

Kang¹,

Lee²,

Lee³

et al. 2014

Korean Journal of Poultry Science

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“…Post-hatch survival rates of around 10% of cultured ova have been obtained with this method (Perry and Mather, 1991) and around 15% using a modification of the method (Naito et al, 1990). Early cleavage embryos obtained prematurely from the uterus by induced oviposition have also been cultured to hatch (Naito and Perry, 1989). The birds reared from cultured embryos were fertile and their reproductive performance was normal.…”

Section: The Early Avian Embryo: Routes For Gene Transfermentioning

confidence: 99%

Transgenesis in chickens

Perry

Sang

1993

Transgenic Research

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The application of transgenic technology to domestic poultry offers an alternative means to conventional practice for improvement of this highly productive agricultural species. The hen's reproductive system has unique characteristics which have imposed limitations on the use of established methods for artificial gene transfer. In this article, we review the various strategies that have been adopted to overcome the problem. Target sites for gene insertion include the fertilized ovum, the blastodermal embryo in the unincubated egg, and the primordial germ cells. Notable success in obtaining somatic and germline transformation has been achieved with the use of retroviral vectors to infect the blastodermal embryo. Current attempts to introduce DNA directly into the genome, without resort to pathogen-derived vectors, are discussed.

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“…2000). This special group of germ cells has been recognized to be the main route for obtaining more detailed mechanism of development of avian gonads during embryonic upgrowth (Naito et al, 1989;1994;Muwana .ct al., 1997;Kagaini et al, 1998;Fujihara and Xi, 2000).…”

Section: Recent Advances In Poultry Reproductionmentioning

confidence: 99%