Transgenesis in chickens

Perry, Margaret M.; Sang, Helen

doi:10.1007/bf01972605

Cited by 27 publications

(6 citation statements)

References 60 publications

(49 reference statements)

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“…2005). However, transgenic chicken production has been hampered by the yolk‐laden structure of the ovum and the unique reproductive system of the birds (Perry and Sang 1993; Sang 1994). Transgenic animals have been successfully generated by injection of foreign DNA into the oocytes, however, this technique is extremely complex and of low efficiency in chicken (Love et al.…”

Section: Discussionmentioning

confidence: 99%

Establishment of the Long‐Term In Vitro Culture System for Chicken Primordial Germ Cells

Shiue

Tailiu

Liou

et al. 2009

Reprod Domestic Animals

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The objective of this study was to establish the long-term in vitro culture system for chicken gonadal primordial germ cells (gPGCs). Primitive gonads collected from 5.5-day-old chicken embryos were dissociated and explanted onto plates pre-coated with 0.1% gelatin. Each of the four different conditioned media from proliferating and mitotically inactivated chicken embryonic fibroblast (CEF) cells and murine embryonic fibroblasts (STO cells, CRL-1053, ATCC, USA), respectively, was supplemented with growth factors and used to support the growth of gPGCs. The result showed that all the conditioned media could promote the growth and colony formation of gPGCs in vitro, in particular the medium conditioned by inactivated CEF cells. The gPGC-derived colonies maintained in inactivated CEF cells-conditioned medium up to 281 days were positively stained by periodic acid Schiff reaction and antibodies specific to anti-SSEA-1, SSEA-3, SSEA-4, integrin alpha6 and integrin beta1. Their capacities of migration via vascular system and taking up residence in the primary gonadal ridge were further demonstrated by transferring to the dorsal aorta of stage 17 recipient embryos. These results suggested that our culture system is able to maintain chicken gPGCs for long-term in vitro culture without losing their capacity to express pluripotent markers and to integrate into the gonads.

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Section: Discussionmentioning

confidence: 99%

Establishment of the Long‐Term In Vitro Culture System for Chicken Primordial Germ Cells

Shiue

Tailiu

Liou

et al. 2009

Reprod Domestic Animals

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“…In the same way as in mammals, the first method developed to transfer genes into birds was microinjection of DNA into the germinal disk of fertilized ova [75]. Although successful germ line transmission has been reported [76], the method is labor-intensive, ineffective, and frequently results in mosaicism.…”

Section: Gene Transfer In Poultrymentioning

confidence: 99%

Transgenic Modification of Production Traits in Farm Animals

Brem¹,

Müller²

2006

Genetically Engineered Food

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“…Mention should also be made of the use of a variant of microinjection in the production of transgenic chickens. This approach has been reviewed in detail elsewhere (Perry & Sang, 1993;Sang, 1994) and consists of microinjecting large quantities of DNA into the cytoplasm of fertilized eggs in the vicinity of the male and female pronuclei and then allowing the eggs to develop using a specialized culture technique. The ®rst generation of chickens produced by this technique are highly mosaic for the integrated DNA but lines of chickens with stable germ-line integrations can be established by normal breeding techniques (Mather et al 1994;Karapetiyan, 1997).…”

Section: Microinjectionmentioning

confidence: 99%

Altering nutrient utilization in animals through transgenesis

Ward

1999

Nutr. Res. Rev.

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Improved domestic animal productivity is necessary in order to provide for an increasing world population over the next two to three decades and such improvement would be aided by an increase in the ef®ciency of nutrient utilization. This can be achieved by conventional genetic selection protocols but progress by this approach is slow. A more rapid but as yet largely unproven technique is the direct modi®cation of the genome which can be achieved by the transfer of recombinant DNA to the nuclei of early embryos. This new technology is potentially powerful because it allows the direct transfer of genes without regard to inter-species barriers to breeding. However, it raises a new set of problems associated with the integration and expression of the foreign genetic information in the new genome. In this review the application of the technology to increasing nutrient utilization and increased productivity are discussed. Two areas have received substantial attention in the 15 years since the technique was ®rst applied to domestic animals. First, the current status of the modi®cation of growth hormone levels to improve productivity and feed utilization ef®ciency is reviewed, with current results suggesting that several of the projects may soon be approaching ®eld trial status.

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Transgenesis in chickens

Cited by 27 publications

References 60 publications

Establishment of the Long‐Term In Vitro Culture System for Chicken Primordial Germ Cells

Establishment of the Long‐Term In Vitro Culture System for Chicken Primordial Germ Cells

Transgenic Modification of Production Traits in Farm Animals

Altering nutrient utilization in animals through transgenesis

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