Genetic Resource Conservation in Poultry Reproduction

Fujihara, Noború; Xi, Yongmei; Zhang, M. J.

doi:10.1080/09712119.2001.9706708

Cited by 4 publications

(2 citation statements)

References 26 publications

(13 reference statements)

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“…This development definitely will endanger the population of indigenous breeds. Fujihara and Xi [4] pointed out genetic diversity is needed by any species in order to maintain reproductive vitality, resistance to disease, and the ability to adapt to changing conditions in the world. The Tsaiya duck, originally domesticated in China, is the major laying duck in Taiwan, which is one of the best duck egg layers in the world [5].…”

Section: Introductionmentioning

confidence: 99%

Monitoring of genetically close Tsaiya duck populations using novel microsatellite markers with high polymorphism

Lai¹,

Chang²,

Chen³

et al. 2020

Asian-Australas J Anim Sci

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Objective: A set of microsatellite markers with high polymorphism from Tsaiya duck were used for the genetic monitoring and genetic structure analysis of Brown and White Tsaiya duck populations in Taiwan.Methods: The synthetic short tandem repeated probes were used to isolate new microsatellite markers from the genomic DNA of Tsaiya ducks. Eight populations, a total of 566 samples, sourced from Ilan Branch, Livestock Research Institute were genotyped through novel and known markers. The population genetic variables were calculated using optional programs in order to describe and monitor the genetic variability and the genetic structures of these Tsaiya duck populations.Results: In total 24 primer pairs, including 17 novel microsatellite loci from this study and seven previously known loci, were constructed for the detection of genetic variations in duck populations. The average values for the allele number, the effective number of alleles, the observed heterozygosity, the expected heterozygosity, and the polymorphism information content were 11.29, 5.370, 0.591, 0.746, and 0.708, respectively. The results of analysis of molecular variance and principal component analysis indicated a contracting Brown Tsaiya duck cluster and a spreading White Tsaiya duck cluster. The Brown Tsaiya ducks and the White Tsaiya ducks with Pekin ducks were just split to six clusters and three clusters when K was set equal to 6 and 3 in the Bayesian cluster analysis. The individual phylogenetic tree revealed eight taxa, and each individual was assigned to its own population.Conclusion: According to our study, the 24 novel microsatellite markers exhibited a high capacity to analyze relationships of inter- and intra-population in those populations with a relatively limited degree of genetic diversity. We suggest that duck farms in Taiwan could use the new (novel) microsatellite set to monitor the genetic characteristics and structures of their Tsaiya duck populations at various intervals in order to ensure quality breeding and conservation strategies.

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Section: Introductionmentioning

confidence: 99%

Monitoring of genetically close Tsaiya duck populations using novel microsatellite markers with high polymorphism

Lai¹,

Chang²,

Chen³

et al. 2020

Asian-Australas J Anim Sci

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“…Also, in a similar study, the motility value was reported as 61.1% (Kasai and Izumo, 2001). It was reported that the contaminants such as blood, feces and lymph fluid coming before or after the semen during semen removal adversely affect the motility and vitality values (Fujihara et al, 2001;Lake, 1983). In addition, a study on ducks reported that artificial vagina method based on natural mating behavior positively affects the motility and vitality values (Kasai and Izumo, 2001).…”

Section: Resultsmentioning

confidence: 78%

Effects of Extenders and Cryoprotectants on Cryopreservation of Duck Semen

Taşkın

Ergün

Karadavut

et al. 2020

Turkish JAF Sci.Tech.

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In this study, it was aimed to determine the usability of different diluents and cry protectants in cryopreservation of Peking duck semen. Semen samples were collected from five male ducks, twice a week for four weeks, using the abdominal massage method. In the study, 40 semen samples were used. Semen samples from ducks were combined (mixed) after being evaluated individually. Mix semen volume (ml), density (×109 / mL), pH, motility (%) and vitality (%) values were determined as 1.84±0.04, 1.72±0.8, 6.88±0.29, 71.55±3.28 and 74.27±4.67 respectively. In the study, as cry protectant, Glucose I (GⅠ) containing 6%DMF (dimethylformamide), Tris-Glucose I (TI), Lactated Ringer's-Glucose I (LGI), Lactated Ringer's I (LI) and Glucose II (GⅠI) containing 5% DMSO, Tris-Glucose II (TII), Lactated Ringer's-Glucose II (LGII), Lactated Ringer's II (LII) were used as diluents. The mixed semen was divided into eight equal parts and 1:3 ratio diluent was added and equilibrated in straws for 90 minutes at + 4°C. The straws were first frozen to -80°C with liquid nitrogen vapour, and then stored in liquid nitrogen at -196°C. After freezing and thawing, the highest motility value was determined in diluent LGI with 41.91%. In other diluents used, the mortality values were determined as LII (33.10%), LGII (32.80%), LI (29.00%), GI (23.66%), TI (12.91%), GII (11.22%) and TII (6.90%). Vitality (%) values were determined as LGI (44.08%), LII (38.50%), LGII (35.10%), LI (33.58), GI (30.91%), GII (23.10%), TI (20.66%) and TII (14.80%) respectively. In this study, it has been determined that the lactated Ringer's-Glucose diluent containing 6% DMF is more advantageous in terms of semen vitality and mortality values when compared to other diluents and that it can be used for long-term storage of duck sperm.

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Вплив Репродуктивного Сезону На Спермопродуктивність Гермінтативних Химер Селезнів

Doroshenko¹,

Chepiha²,

Kostenko³

et al. 2018

Anim. Breed. and Genet.

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У статті наведені результати дослідження спермопродуктивності селезнів порід шаосінь, шанма та герментативних химер. Було встановлено, що за об’ємом еякуляту, концентрацією та активністю сперматозоїдів герментативні химери переважали селезнів порід шаосінь (Р < 0,05) та шанма (Р < 0,01). Результати дисперсійного аналізу свідчать, що частка впливу породи на об’єм еякуляту становить 25%, а періоду репродуктивного сезону – 13%. Спермопродуктивність гермінативних химер показує, що їх можливо використивувати у біотехнологічних процедурах по створенню трансгенних качок.

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Genetic Resource Conservation in Poultry Reproduction

Cited by 4 publications

References 26 publications

Monitoring of genetically close Tsaiya duck populations using novel microsatellite markers with high polymorphism

Monitoring of genetically close Tsaiya duck populations using novel microsatellite markers with high polymorphism

Effects of Extenders and Cryoprotectants on Cryopreservation of Duck Semen

Вплив Репродуктивного Сезону На Спермопродуктивність Гермінтативних Химер Селезнів

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