Development, growth, propagation, and angiographic utilization of the rabbit VX2 model of liver cancer: a pictorial primer and “how to” guide

Parvinian, Ahmad; Casadaban, Leigh C.; Gaba, Ron C.

doi:10.5152/dir.2014.13415

Cited by 65 publications

(41 citation statements)

References 27 publications

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“…The VX2 line is quite cumbersome to use, as a culture protocol has not been established and the cells have to be propagated as muscle implants into syngeneic (New Zealand White) rabbits. Tumors are then dissected and implanted into syngeneic recipient target organs [19]. VX2 is characterized by very rapid, aggressive growth and hypervascularity, but with a high rate of spontaneous necrosis of tumor cells [19].…”

Section: Discussionmentioning

confidence: 99%

“…Tumors are then dissected and implanted into syngeneic recipient target organs [19]. VX2 is characterized by very rapid, aggressive growth and hypervascularity, but with a high rate of spontaneous necrosis of tumor cells [19]. Although it has been suggested that there are similarities between glioma and VX2, the clinical relevance of VX2 as a primary brain tumor model is limited, and should be rather considered as a model of brain metastatic tumor.…”

Section: Discussionmentioning

confidence: 99%

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Rabbit Model of Human Gliomas: Implications for Intra-Arterial Drug Delivery

et al. 2017

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The prognosis for malignant brain tumors remains poor despite a combination of surgery, radiotherapy, and chemotherapy. This is partly due to the blood-brain barrier, a major obstacle that prevents therapeutic agents from effectively reaching the tumor. We have recently developed a method for precise and predictable opening of the blood-brain barrier via the intra-arterial administration of mannitol, a hyperosmolar agent, in a rabbit model, whose vascular anatomy facilitates the use of standard interventional neuroradiology techniques and devices. To date, however, no protocols are available that enable human glioma modeling in rabbits. In this article, we report on the xenotransplantation of a human glioblastoma (GBM-1) in adult New Zealand rabbits. We induced multi-drug immunosuppression (Mycophenolate Mofetil, Dexamethasone, Tacrolimus) and stereotactically implanted GBM-1 tumor cells into rabbit brains. The rabbits were followed for 42 days, monitored by MRI and body weight measurements, and underwent postmortem histopathological analysis. On MRI, brain tumors were identified on T2-weighted scans. On histopathology, tumors were detected with hematoxylin/eosin and their human origin was confirmed with immunohistochemistry against human-specific antigens. Our method for human glioma modeling in rabbits provides the foundation to test novel treatment strategies, including intra-arterial therapeutic agent delivery.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Rabbit Model of Human Gliomas: Implications for Intra-Arterial Drug Delivery

et al. 2017

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“…Subsequently, skin preparation and an incision of 4–5 cm were made on the right hind limb until reaching the surrounding tumor area. Two curvilinear incisions were made within the muscle, in order to remove the tumor mass en-bloc as described in Parvivian et al[16]. The explanted tumor was bisected in order to detect any gross evidence of a necrotic core to obtain viable peripheral tumor.…”

Section: Methodsmentioning

confidence: 99%

“…It is of note that this model has been used to model various types of cancer, including those of pancreas, kidney and liver [5–15]. The transplantation of VX2 cells can be achieved either by implanting solid tumor pieces or by injecting the tumor cell suspensions (fresh or frozen) as previously described [16]. …”

Section: Introductionmentioning

confidence: 99%

Induction of VX2 para-renal carcinoma in rabbits: generation of animal model for loco-regional treatments of solid tumors

Bimonte

Leongito

Piccirillo

et al. 2016

Infect Agents Cancer

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BackgroundAnimal models of para-renal cancer can provide useful information for the evaluation of tumor response to loco-regional therapy experiments in solid tumors. The aim of our study was to establish a rabbit para-renal cancer model using locally implanted VX2 tumors.MethodsIn order to generate a rabbit model of para-renal cancer, we established four hind limb donor rabbits by using frozen VX2 tumor samples. Following inoculation, rabbits were monitored for appetite and signs of pain. Viable tumors appeared as palpable nodules within 2 weeks of inoculation. Tumor growth was confirmed in all rabbits by high-resolution ultrasound analysis and histology. Once tumor growth was established, hind limb tumors extraction was used for tumor line propagation and para-renal tumor creation. Twenty-one rabbit models bearing para-renal cancer were established by implanting VX2 tumor into the para-renal capsula. Tumors developed into discreet 2–3 cm nodules within 1–3 weeks of implantation. Serial renal ultrasonography follow-up, starting 1 week after tumor implantation, was performed. Two weeks after tumor implantation, rabbits were euthanized and tumors and other organs were collected for histopathology.ResultsTumor growth after VX2 tumor fragment implantation was confirmed in all rabbits by high-resolution ultrasound (US) imaging examinations of the para-renal regions and was measured with digital caliper. The para-renal injection of VX2 tumor fragments, achieved tumor growth in 100% of cases. All data were confirmed by histological analysis.ConclusionsWe generated for the first time, a model of para-renal cancer by surgical tumor implantation of VX2 frozen tumor fragments into rabbit’s para-renal region. This method minimizes the development of metastases and the use of non-necrotic tumors and will optimize the evaluation of tumor response to loco-regional therapy experiments.

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“…Преимущественно используется опухоль VX2 (плоскоклеточная карцинома). Перевивку этой опухоли применяют в экспериментальной онкологии для моделирования злокачественных новообразований головы и шеи, почки, головного мозга, печени [62], при исследовании колоректального рака с метастазами в печень [67]. Для исследования образования метастазов лимфатическая система кролика подходит больше, чем у грызунов [61].…”

Section: трансгенные и нокаутные кролики в биомедицинеunclassified

Rabbit Biomodels of Human Diseases Developed Using New Genomic Technologies. Crispr/Cas9 (Review)

et al. 2019

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With the advent of endonuclease methods of genome editing, particularly CRISPR/Cas9, it has become possible to obtain genetically modified rabbits by microinjection of zygotes. These highly effective human disease models can be used for various purposes. The present review aims to consider modern achievements in the creation of rabbit biomodels of human diseases using the technologies of genetic editing. It is concluded that Russian laboratories should intensify research in the development of genetically modified rabbits that can be used for various biomedical studies and biomodelling.

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Development, growth, propagation, and angiographic utilization of the rabbit VX2 model of liver cancer: a pictorial primer and “how to” guide

Cited by 65 publications

References 27 publications

Rabbit Model of Human Gliomas: Implications for Intra-Arterial Drug Delivery

Rabbit Model of Human Gliomas: Implications for Intra-Arterial Drug Delivery

Induction of VX2 para-renal carcinoma in rabbits: generation of animal model for loco-regional treatments of solid tumors

Rabbit Biomodels of Human Diseases Developed Using New Genomic Technologies. Crispr/Cas9 (Review)

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