Rabbit Biomodels of Human Diseases Developed Using New Genomic Technologies. Crispr/Cas9 (Review)

Abstract

With the advent of endonuclease methods of genome editing, particularly CRISPR/Cas9, it has become possible to obtain genetically modified rabbits by microinjection of zygotes. These highly effective human disease models can be used for various purposes. The present review aims to consider modern achievements in the creation of rabbit biomodels of human diseases using the technologies of genetic editing. It is concluded that Russian laboratories should intensify research in the development of genetically modified rabbits that can be used for various biomedical studies and biomodelling.

Conflict of interest: the authors declare no conflict of interest.

“…Появление эндонуклеазных методов и технологий редактирования генома позволило получать крупных генетически модифицированных животных -кроликов. Более ранний метод получения таких животных -классический трансгенез -был низкоэффективным [4].…”

Genetically modified rabbits as bio-producers and biomodels

“…In recent years, using CRISPR/Cas9 technology there were produced GM rabbitsmodels of such human diseases as: cardiovascular (hypertrophic cardiomyopathy, atherosclerosis, arterial thrombosis); immune diseases, including immunodeficiency; infectious diseases; metabolic syndromes due to both nutrition and heredity; Xchromosome-related diseases -hypophosphatemia (a common cause of inherited rickets) and Duchenne Muscular Dystrophy; progeria syndrome; hermaphroditism; ectodermal dysplasia; cleft palate; Wilson's disease (copper metabolism disorder, leading to severe hereditary diseases of the Central nervous system and internal organs); models were created to study the metabolism of lipids and lipoproteins [14].…”