Development and validation of liquid chromatography–tandem mass spectrometry method for simultaneous determination of six steroidal saponins in rat plasma and its application to a pharmacokinetics study

Liu, Zhirui; Qin, Wenxing; Zhu, Zhenyu; Liu, Yao; Sun, Fengjun; Chai, Yifeng; Xia, Peiyuan

doi:10.1016/j.steroids.2015.01.006

Cited by 15 publications

(10 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The plasma concentration-time curves of timosaponin BII, anemarsaponin BIII and timosaponin E1 after oral administration showed a double peak, which was also reported in [23,24,25]. It is well known that drug absorption is a very complex process that manifests itself through potential interaction with a host of physicochemical and physiological variables.…”

Section: Resultssupporting

confidence: 61%

Anemarrhena asphodeloides Non-Steroidal Saponin Components Alter the Pharmacokinetic Profile of Its Steroidal Saponins in Rat

Tang

Yang

et al. 2015

Molecules

View full text Add to dashboard Cite

A rapid, selective and sensitive UPLC-MS/MS assay was established to determine the plasma concentrations of four steroidal saponins. Sprague-Dawley rats were allocated to four groups which were orally administered Anemarrhena asphodeloides extracts (ASE), ASE combined with macromolecular fraction (ASE-MF), ASE combined with small molecule fraction (ASE-SF) and ASE combined with small molecule and macromolecular fraction (ASE-SF-MF) containing approximately the same dose of ASE. At different time points, the concentration of timosaponin BII, anemarsaponin BIII, timosaponin AIII and timosaponin E1 in rat plasma were determined and main pharmacokinetic parameters including Cmax, Tmax, T1/2, AUC were calculated using the DAS 3.2 software package. The statistical analysis was performed using the Student's t-test with p < 0.05 as the level of significance. MF had no effect on the pharmacokinetic behaviors and parameters of four steroidal saponins. It was found that Cmax and AUC of four steroidal saponins in group ASE-SF and ASE-SF-MF, were significantly increased compared with those in group ASE. OPEN ACCESSMolecules 2015, 20 11778These results indicate that SF in A. asphodeloides extracts could increase the absorption and improve the bioavailability of the steroidal saponins.

show abstract

Section: Resultssupporting

confidence: 61%

Anemarrhena asphodeloides Non-Steroidal Saponin Components Alter the Pharmacokinetic Profile of Its Steroidal Saponins in Rat

Tang

Yang

et al. 2015

Molecules

View full text Add to dashboard Cite

show abstract

“…As seen in Figure 3, neomangiferin and mangiferin reached C max at around 4-6 h after oral administration, indicating that these two xanthones had a moderate absorption rate. Distinct double peaks were observed in the mean plasma concentration-time curves of timosaponin BII and timosaponin BIII, and this finding is in agreement with that of previous studies [25,27]. The first peak was observed at about 10 min after oral administration, indicating that timosaponin BII and timosaponin BIII could be quickly absorbed into the blood circulatory system.…”

Section: Pharmacokinetic Studysupporting

confidence: 92%

“…Therefore, both xanthones and steroidal saponins can be used as marker constituents of AR in pharmacokinetic studies. During the last decade, different analytical techniques including LC coupled with different detectors, such as LC-UV [17,18], LC-MS [19,20], and LC-MS/MS [21][22][23][24][25][26][27][28] have been described for the quantitative determination of xanthones and steroidal saponins in AR in various biological specimens. Among these studies, only two publications established analytical methods capable of simultaneously analyzing xanthones and steroidal saponins [22,28].…”

Section: Introductionmentioning

confidence: 99%

A novel ultra high‐performance liquid chromatography‐tandem mass spectrometry method for the simultaneous determination of xanthones and steroidal saponins in crude and salt‐processed Anemarrhenae Rhizoma aqueous extracts

Huang

et al. 2018

J of Separation Science

View full text Add to dashboard Cite

We established a rapid and sensitive ultra high-performance liquid chromatography tandem mass spectrometry method for the simultaneous quantification of xanthones and steroidal saponins in rat plasma. Chromatographic separation was achieved on a C column with a mobile phase comprising acetonitrile and 0.1% formic acid. The detection was performed by negative electrospray ionization in multiple reaction monitoring mode. The validated method showed good linearity within the tested range (r > 0.9945). The intra- and interday precision at high, medium, and low concentrations was less than 7.96%. The bias of accuracies ranged from -1.92 to 9.62%. The extraction recoveries of the compounds ranged from 84.78 to 88.69%, and the matrix effects ranged from 96.76 to 108.59%. This method was successfully applied to a pharmacokinetic comparison of crude and salt-processed Anemarrhenae Rhizoma aqueous extracts after oral administration in rats. The maximum plasma concentration and area under concentration-time curve of timosaponin BIII and timosaponin AIII increased significantly (P < 0.05 or 0.01) and those of timosaponin BII decreased significantly (P < 0.05) after processing. These results could contribute to the clinical application of crude and salt-processed Anemarrhenae Rhizoma and reveal the processing mechanism.

show abstract

“…Based on chromatographic theory, ultra‐performance liquid chromatography (UPLC), compared with traditional HPLC, which provides higher peak capacity, greater resolution and increased sensitivity, is widely accepted as an ideal separation tool (Liu etal. , ; Lu etal. , ; Wu etal., ).…”

Section: Introductionmentioning

confidence: 99%

Development and validation of an UPLC‐Q/TOF‐MS assay for the quantitation of neopanaxadiol in beagle dog plasma: Application to a pharmacokinetic study

Geng

Wang

et al. 2016

Biomedical Chromatography

View full text Add to dashboard Cite

Neopanaxadiol (NPD), the main panaxadiol constituent of Panax ginseng C. A. Meyer (Araliaceae), has been regarded as the active component for the treatment of Alzheimer's disease. However, few references are available about pharmacokinetic evaluation for NPD. Accordingly, a rapid and sensitive method for quantitative analysis of NPD in beagle dog plasma based on ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry was developed and validated. Analytes were extracted from plasma by liquid-liquid extraction and chromatographic separation was achieved on an Agilent Zorbax Stable Bond C column. Detection was performed in the positive ion mode using multiple reaction monitoring of the transitions both at m/z 461.4 → 425.4 for NPD and internal standard of panaxadiol. All validation parameters, such as lower limit of quantitation, linearity, specificity, precision, accuracy, extraction recovery, matrix effect and stability, were within acceptable ranges and the method was appropriate for multitude sample determination. After oral intake, NPD was slowly absorbed and eliminated from circulatory blood system and corresponding plasma exposure was low. Application of this quantitative method will yield the first pharmacokinetic profile after oral administration of NPD to beagle dog. The information obtained here will be useful to understand the pharmacological effects of NPD.

show abstract

Development and validation of liquid chromatography–tandem mass spectrometry method for simultaneous determination of six steroidal saponins in rat plasma and its application to a pharmacokinetics study

Cited by 15 publications

References 15 publications

Anemarrhena asphodeloides Non-Steroidal Saponin Components Alter the Pharmacokinetic Profile of Its Steroidal Saponins in Rat

Anemarrhena asphodeloides Non-Steroidal Saponin Components Alter the Pharmacokinetic Profile of Its Steroidal Saponins in Rat

A novel ultra high‐performance liquid chromatography‐tandem mass spectrometry method for the simultaneous determination of xanthones and steroidal saponins in crude and salt‐processed Anemarrhenae Rhizoma aqueous extracts

Development and validation of an UPLC‐Q/TOF‐MS assay for the quantitation of neopanaxadiol in beagle dog plasma: Application to a pharmacokinetic study

Contact Info

Product

Resources

About