Development and validation of bioanalytical method for the determination of hydrazinocurcumin in rat plasma and organs by HPLC-UV

Satyavert,; Gupta, Sumeet; Nair, Anroop B.; Attimarad, Mahesh

doi:10.1016/j.jchromb.2020.122310

Cited by 14 publications

(9 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The linearity (r 2 > 0.999) of HZC was found in the range 0.05-5 μg/mL with a retention time of 8 min for HZC and 4 min for IS. The developed bioanalytical method using plasma and various tissue samples was found to be accurate and precise, which had been established in intraday and inter-day accuracy and precision [23]. The lower limit of quantification of HZC in plasma and tissues was 0.025 μg/mL.…”

Section: Bioanalytical Methodsmentioning

confidence: 97%

“…Quantitative estimation of HZC in plasma and various organ samples was carried out by our developed and validated bioanalytical method using high-performance liquid chromatography (HPLC) system (Shimadzu LC-10, Kyoto, Japan) [23]. Photodiode array detector used for identifying the analytes and LC solution software (Version; 7.1, Japan) was employed for chromatographic evaluation.…”

Section: Bioanalytical Methodsmentioning

confidence: 99%

“…HZC was separated by C 18 Inertsil ® ODS −3 V with a guard column Lichrospher (Merck, Germany) and an isocratic mobile phase. The mobile phase was consisting of methanol: acetonitrile: water: 3.6:2.7:3.7, which was maintained at a flow rate of 1 mL/min [23]. The sample injection volume was 50 μL and detection of HZC and internal standard (IS) (p-nitro aniline) was carried out in dual-wavelength mode at 332 and 380 nm, respectively.…”

Section: Bioanalytical Methodsmentioning

confidence: 99%

“…Even though various biological activities with enhanced pharmacological role have been documented for HZC; however, very few pharmacokinetic studies with detailed analysis have been reported in the literature [22,23]. Therefore, pharmacokinetics and tissue distribution studies of HZC in animal models following administration via different routes of administration would shed light on the prospect of modulating the bioavailability of HZC and ensuing biological activity based on the structural modifications.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Pharmacokinetics and tissue distribution of hydrazinocurcumin in rats

Satyavert¹,

Gupta²,

Choudhury

et al. 2021

Pharmacol. Rep

Self Cite

View full text Add to dashboard Cite

Background Curcumin, a natural polyphenol from Curcuma longa, is known to possess diversified pharmacological roles including anti-inflammatory, antioxidant, antiproliferative and antiangiogenic properties; however, its bioavailability is severely limited due to its poor solubility, poor absorption, rapid metabolism, and significant elimination. Hydrazinocurcumin (HZC), a novel analogue of curcumin has been reported to overcome the limitations of curcumin and also possesses multiple pharmacological activities. The present study aimed to evaluate the unexplored pharmacokinetic profile of this agent in experimental rats. Methods Drug formulations were administered to the experimental animals via oral, intravenous and intraperitoneal routes. Blood samples were collected at different pre-determined time intervals to determine the pharmacokinetic parameters. To understand the biodistribution profile of HCZ, tissue samples were isolated from different groups of Sprague-Dawley rats at different time points. The pharmacokinetic parameters of HZC were evaluated after administration through oral (100 mg/ kg), intraperitoneal (100 mg/kg) and intravenous (10 mg/kg) routes. Results Significantly (p < 0.05) higher total AUC along with maximum concentration were evident with intraperitoneal administration when compared to the results of oral administration at a similar dose. In addition, shorter time to peak was observed with intraperitoneal administration. These results revealed a faster rate and longer duration of absorption with intraperitoneal administration, which further resulted in enhanced absolute bioavailability of HZC (29.17%) when compared to 5.1% upon oral dosing. The obtained data from the pharmacokinetic study indicated that HZC was instantaneously distributed and moderately eliminated from body fluids. Conclusion Based on the findings, it could be concluded that absorption of HZC is much higher via intraperitoneal route of administration compared to the oral administration.

show abstract

Section: Bioanalytical Methodsmentioning

confidence: 97%

Section: Bioanalytical Methodsmentioning

confidence: 99%

Section: Bioanalytical Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Pharmacokinetics and tissue distribution of hydrazinocurcumin in rats

Satyavert¹,

Gupta²,

Choudhury

et al. 2021

Pharmacol. Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…The organs viz. lungs, liver, and spleen were harvested from the animals to study the targeting efficiency [32]. Dexamethasone was extracted from the harvested organs.…”

Section: Pharmacokinetic Biodistribution and Targeting Efficiencymentioning

confidence: 99%

Lung Targeted Lipopolymeric Microspheres of Dexamethasone for the Treatment of ARDS

et al. 2021

Self Cite

View full text Add to dashboard Cite

Acute respiratory distress syndrome (ARDS), a catastrophic illness of multifactorial etiology, involves a rapid upsurge in inflammatory cytokines that leads to hypoxemic respiratory failure. Dexamethasone, a synthetic corticosteroid, mitigates the glucocorticoid-receptor-mediated inflammation and accelerates tissue homeostasis towards disease resolution. To minimize non-target organ side effects arising from frequent and chronic use of dexamethasone, we designed biodegradable, lung-targeted microspheres with sustained release profiles. Dexamethasone-loaded lipopolymeric microspheres of PLGA (Poly Lactic-co-Glycolic Acid) and DPPC (Dipalmitoylphosphatidylcholine) stabilized with vitamin E TPGS (D-α-tocopheryl polyethylene glycol succinate) were prepared by a single emulsion technique that had a mean diameter of 8.83 ± 0.32 μm and were spherical in shape as revealed from electron microscopy imaging. Pharmacokinetic and biodistribution patterns studied in the lungs, liver, and spleen of Wistar rats showed high selectivity and targeting efficiency for the lung tissue (re 13.98). As a proof-of-concept, in vivo efficacy of the microspheres was tested in the lipopolysaccharide-induced ARDS model in rats. Inflammation markers such as IL-1β, IL-6, and TNF-α, quantified in the bronchoalveolar lavage fluid indicated major improvement in rats treated with dexamethasone microspheres by intravenous route. Additionally, the microspheres substantially inhibited the protein infiltration, neutrophil accumulation and lipid peroxidation in the lungs of ARDS bearing rats, suggesting a reduction in oxidative stress. Histopathology showed decreased damage to the pulmonary tissue upon treatment with the dexamethasone-loaded microspheres. The multipronged formulation technology approach can thus serve as a potential treatment modality for reducing lung inflammation in ARDS. An improved therapeutic profile would help to reduce the dose, dosing frequency and, eventually, the toxicity.

show abstract

Validation of a bioanalytical HPLC–UV method to quantify Α‐Bisabolol in rat plasma applied to pharmacokinetic pilot study with the drug nanoemulsion

da Hora Borges,

Santos de Araújo,

Pereira

et al. 2024

Biomedical Chromatography

View full text Add to dashboard Cite

Abstractα‐Bisabolol (α‐BIS) is a sesquiterpene alcohol present in chamomile essential oil [Chamomilla recutita (L.) Rauschert]. Despite its numerous pharmacological effects, its pharmacokinetics remain understudied. An analytical method capable of quantifying α‐BIS in plasma is crucial to enable pharmacokinetic analysis. Presently, only one study has quantified it using mass spectrometry. Administering α‐BIS requires a nanoemulsion for intravenous injection. This study aimed to develop and validate a bioanalytical method using high‐performance liquid chromatography with an ultraviolet detector to quantify α‐BIS in rat plasma. The method employed acetonitrile and ultrapure water (80:20, v/v) as the mobile phase, with a flow rate of 1 ml/min and concentrations ranging from 465 to 29.625 μg/ml. All US Food and Drug Administration‐designated assays were successful, indicating the method's precision, accuracy, sensitivity and linearity in determining α‐BIS in rat plasma. The developed nanoemulsion, assessed through dynamic light scattering analysis, the ensemble collection of particles and polydispersity index evaluation, proved safe and effective for intravenous administration. The pharmacokinetic parameters such as volume of distribution, clearance and half‐life indicated that α‐BIS tends to persist in the body. This study provides a foundation for further research to explore α‐BIS's potential pharmaceutical applications in the future.

show abstract

Development and validation of bioanalytical method for the determination of hydrazinocurcumin in rat plasma and organs by HPLC-UV

Cited by 14 publications

References 26 publications

Pharmacokinetics and tissue distribution of hydrazinocurcumin in rats

Pharmacokinetics and tissue distribution of hydrazinocurcumin in rats

Lung Targeted Lipopolymeric Microspheres of Dexamethasone for the Treatment of ARDS

Validation of a bioanalytical HPLC–UV method to quantify Α‐Bisabolol in rat plasma applied to pharmacokinetic pilot study with the drug nanoemulsion

Contact Info

Product

Resources

About