Development and validation of analytical methods by HPLC for quality control of avermectins and milbemycins in bulk samples

Abstract

This work aims to develop analytical methods using high-performance liquid chromatography with a diode array detector (HPLC-DAD) for analysis and quantification of avermectins (AVMs) and milbemycins (MBMs) in bulk samples. First, the methods were optimized and some parameters such as temperature, flow rate, injection volume and mobile phase with different proportions of solvents were evaluated. The best chromatographic conditions were obtained using the Phenomenex ® C18 (150 × 4.60 mm, 5 μm) column at a temperature of 20 °C, flow rate of 1.2 mL min -1 , injection volume of 20 μL, and detection at 250 nm. Acetonitrile: ultrapure water (87: 13, v/v) was used as mobile phase for moxidectin and eprinomectin, and acetonitrile: methanol: ultrapure water (53: 35: 12, v/v/v) for abamectin and ivermectin. Under these conditions satisfactory results were obtained, with appropriate limits of detection and quantification, acceptable linearity, precision, accuracy, and robustness. These methods satisfy the need for analytical methods for the multi-determination of MBMs and the B1a and B1b forms of AVMs by HPLC-DAD, which can be considered simple, effective, innovative and should aid in the development of the field.

Validation and Applicability of an HPLC Method for Analysis of Eprinomectin in Responsive Systems for Pour-on Administration in Cattle

“…The chromatographic analyses were performed using an HPLC system [27][28][29][30] model Alliance 2696 (Waters ® , Wexford, Ireland), equipped with a C18 reversed phase column (4.6 x 75mm, 3.5µm) (Symmetry Waters ® , Wexford, Ireland), a photodiode array detector (Waters ® , Wexford, Ireland) and the Empower 3 ® software (Waters ® , Milford, MA, USA). The study was performed using isocratic elution of mobile phase comprised of acetonitrile:methanol:water (47:33:20, v/v/v), flow of 1.5 mL/min, and at the temperature of 30 ºC.…”

“…However, no study reported the application of the method for quantification of the active in a polymeric system (containing Poloxamer P407, Carbopol C974P and isopropanol) for veterinary use. Most studies are applied to detection in milk [27,28]. Therefore, the aim of this work was to develop and validate a high-performance liquid chromatographic (HPLC) method for analysis of EPR from the polymeric systems.…”

Quantitative Estimation of Rivaroxaban in Bulk and Pharmaceutical Formulation using High-Pressure Liquid Chromatography in Reverse Phase

“…Its molecular weight is 435.89 g/mol, and its IUPAC nomenclature is 5-chloro-N-[(5S) [4-(3oxomorpholin -4-yl) phenyl]-2-oxo-3-Thiophene-2carboxamide, or [-1, 3-oxazolidin-5-yl]methylthiophene [1][2][3]. It belongs to a class of an anticoagulant [4]. It is used in the treatment of deep venous thrombosis.…”