Development and Validation of an Ecological, New and Rapid Stability-Indicating High Performance Liquid Chromatography for Quantitative Determination of Aztreonam in Lyophilized Powder for Injection

Abstract

Aztreonam is a monocyclic synthetic antimicrobial with bactericidal activity against Gram-negative bacteria, the first agent from the monobactam family to be therapeutically approved. It was developed and validated analytical method using high performance liquid chromatography with UV detection at 292 nm to quantify the aztreonam. Furthermore, assessing stability through stress tests was carried out. The chromatographic separation was carried out by reverse phase on an Agilent C18 column (250 x 4.6 mm, 5 µm) with a mobile phase composed of water:ethanol (70:30, v/v) adjusted to pH 2.5 with acetic acid, pumped isocratically at a flow rate of 0.5 mL/min. The validation parameters linearity, selectivity, precision, accuracy, robustness, limits of detection and quantification were determined. The method proposed provides linear response within the concentration range of 45-95 µg/mL for aztreonam. Results obtained were found to be satisfactory. The proposed method is linear, accurate, precise, selective, and robust being able to quantify the aztreonam in raw material and in pharmaceutical preparations. The validated method was suitable for applications in quality control laboratories.

Eco-Friendly Green Liquid Chromatographic for Determination of Doxycycline in Tablets and in the Presence of Its Degradation Products

“…Besides that, ethanol is a biodegradable solvent, the environmental impacts are minimized and can be easily replaced and reused because it is the least toxic (19). Currently, the use of water and ethanol as mobile phase is already a reality; methods to determine drugs such as sodium cefepime hydrochloride (20), ceftriaxone sodium (21), cefadroxil (22), daptomycin (23), ampicillin (24), cefazolin sodium (25) aztreonam (26) and rifaximin (27) Drug Anal Res, 2017; 02, 49-55 were developed and validated using green analytical chemistry. In addition, DOX has highly solubility in water, which it was possible to prepare the samples in aqueous medium, further contributing to green analytical chemistry.…”

Evolution of green chemistry and its multidimensional impacts: A review

“…Lack of knowledge, negligence of consequences, ease and/or accommodation? (Tótoli and Salgado, 2014, Pedroso et al, 2016, Spagnol et al, 2016, Rodrigues and Salgado, 2016, Figueiredo et al, 2017, Kogawa et al, 2017, Marco and Salgado, 2017).…”

A Critical Review of Analytical Methods for Determination of Ceftriaxone Sodium

“…Many methods use buffer solutions that are non-toxic to the environment and the operator, but can shorten the life of the equipment and accessories, for example, the chromatographic columns, impacting the cost of the analysis. [60][61] It is necessary to develop methods that use solvents with low toxicity (ethanol and water, for example), as well as to use them in low concentrations. The commitment to using reduced samples through miniaturization, decreasing process steps and pretreatment of samples, are alternatives that directly influence the amount of reagents used, time of analysis, number of materials required and cost involved.…”