Development and Validation of an HPLC Method for the Determination of Dexamethasone, Dexamethasone Sodium Phosphate and Chloramphenicol in Presence of Each Other in Pharmaceutical Preparations

Iqbal, Mohammad S.; Shad, Muhammad Aslam; Ashraf, Muhammad Waqar; Bilal, Muhammad; Saeed, Maryiam

doi:10.1365/s10337-006-0019-3

Cited by 30 publications

(12 citation statements)

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“…Dexamethasone (8S,9R,10S,11S,13S,14S,1 6R,17R)-9-Fluoro-11,17-dihydroxy-17-(2-hydroxyacetyl)-10,13,16-trimethyl-, 7,8,9,10,11,12,13,14,15,16,17-dodecahydro-3H-cyclopenta[a] phenanthren-3-one [ Figure 1] a corticosteroid, is used to relieve bacterial ocular infections that require corticosteroid [1]. Many authors have reported a lot of different analytical methods for both drugs individually utilizing different techniques like HPLC [2][3][4][5][6][7][8][9], spectrophotometry and spectrofluorometric [10][11][12][13], titrimetric [14] methods for ciprofloxacin while for dexamethasone different methods involving HPLC, GC and TLC are reported [15][16][17][18][19][20][21].…”

Section: Ciprofloxacinmentioning

confidence: 99%

Stability Indicating Rp-HPLC Method for Simultaneous Determination of Ciprofloxacin and Dexamethasone in Binary Combination

Razzaq

Ashfaq

Mariam³

et al. 2017

J. Chil. Chem. Soc.

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A simple and isocratic HPLC method with stability indicating nature was developed and then subsequently validated for simultaneous determination of ciprofloxacin and dexamethasone in pharmaceutical formulations, human serum and urine. Best chromatographic separations were attained within run time of 10 minutes using C8 as stationary phase and mixture of phosphate buffer and methanol (41:59 v/v) as mobile phase. The mobile phase was flowed at 1.5 mL min -1 with detection of both the analytes at 270 nm using photodiode array detector. Validation of the method was accomplished using specificity, linearity, accuracy, precision, robustness, LOD and LOQ. The method was found linear from 3-21 mg mL -1 for ciprofloxacin (r 2 ≥ 0.999) and 1-7 mg mL -1 for dexamethasone (r 2 ≥ 0.999). The %age recoveries of ciprofloxacin in spiked human urine and serum were ≥99% and ≥85% respectively, while for dexamethasone they were ≥97% in both matrices. The method proficiently separated the peaks of ciprofloxacin and dexamethasone from all types of interfering substances including degradation products/impurities with purity index ≥ 0.9998. The method thus was stability-indicating and can be employed for simultaneous analysis of ciprofloxacin and dexamethasone in complex matrices involving multiple components in the mixture. 1-INTRODUCTION Ciprofloxacin(1-cyclopropyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-quinoline-3-carboxylic acid), a well-known antibiotic, is used to relieve bacterial infections of the eyes, corneal ulcers along with some common bacterial attacks. Dexamethasone (8S,9R,10S,11S,13S,14S,1 6R,17R)-9-Fluoro-11,17-dihydroxy-17-(2-hydroxyacetyl)-10, 13,16-trimethyl-,7,8,9,10,11,12,13,14,15,16,17-dodecahydro-3H-cyclopenta[a] phenanthren-3-one [ Figure 1] also the enforcement agencies in general to monitor the quality of the marketed products. 2-EXPERIMENTAL Chemicals and ReagentsCiprofloxacin (99.87%) and dexamethasone (99.96%) reference standards were received from Schazoo Zaka Laboratories (Lahore, Pakistan) and were used as such without further refinement. Commercial products (Zoxan D and Ciprodex) containing 3 mg mL -1 ciprofloxacin and 1 mg mL -1 dexamethasone were analyzed during the current research work. All the chemicals used were of either HPLC grade or highest grade available commercially and were used as such. Double distilled water, other liquids used in mobile phase and all the injecting solutions were subject to filtration employing 0.45 mm nylon filters (Millipore, USA). Equipment and Chromatographic ConditionsAll the chromatographic work was conducted on LC-20A system (Shimadzu, Japan) using PDA detector at 270 nm. Each time 20 mL volume of analytes were injected using the fixed loop and peak areas of ciprofloxacin and dexamethasone were integrated using system built software. For separation, Hypersil BDS C8 column (250 X 4.6 mm, 5 mm) was used at room temperature. Pre-mixed mobile phase was used which was prepared by mixing 59:41 v/v ratio of methanol and 0.018M phosphate buffer (pH 3.0) and was flowe...

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Section: Ciprofloxacinmentioning

confidence: 99%

Stability Indicating Rp-HPLC Method for Simultaneous Determination of Ciprofloxacin and Dexamethasone in Binary Combination

Razzaq

Ashfaq

Mariam³

et al. 2017

J. Chil. Chem. Soc.

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“…The determination of gatifloxacin has widely been reported using spectrophotometry (Sonali et al, 2006;Venugopal, Ranendra, 2005), HPLC (Santoro et al, 2006), atomic absorption spectrophotometry, conductometry, colorimetry (Sheikh, 2008) and spectrofluorimetry (Ocaña, Barragán, Callejón, 2005). Similarly, the estimation of dexamethasone has also been reported widely by HPLC (Iqbal et al, 2006;Huetos et al, 1999; gap, the present study was designed to optimize the chromatographic conditions in order to estimate them in different matrices (pharmaceutical formulations, human urine and serum). In the past few years, different FDC have already been reported by our research group after developing suitable analytical methods Khan et al, 2013;Razzaq et al, 2013;John et al, 2015a, b;Saleem et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination

Razzaq

Ashfaq

Khan

et al. 2017

Braz. J. Pharm. Sci.

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In this study, conditions were optimized for development of a simple RP-HPLC method for simultaneous analysis of gatifloxacin and dexamethasone in different matrices like pharmaceuticals, human serum and urine. Good separation of gatifloxacin and dexamethasone from the induced degradation products was accomplished using C8 as stationary phase; 0.02 M phosphate buffer (pH 3. .999). Both the analyte peaks were completely separated from the peaks of induced degradation products as indicated by the peak purity index (≥0.9999 for both analytes). The optimized method is recommended to be used for concurrent analysis of gatifloxacin and dexamethasone in different matrices.

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“…6 The literature survey revealed that few methods determined simultaneously Chloramphenicol and Dexamethasone Sodium Phosphate 8,9 in the presence of Dexamethasone. 10 Therefore, the aim of this work was to develop and validate a new simple stability-indicating HPLC method for simultaneous determination of Chloramphenicol, its hydrolysis derivative (AMPD), Dexamethasone sodium phosphate, its hydrolysis derivative (Dexamethasone) and Tetrahydrozoline in the ophthalmic solution.…”

Section: Introductionmentioning

confidence: 99%

Stability-Indicating HPLC Method for Simultaneous Determination of Chloramphenicol, Dexamethasone Sodium Phosphate and Tetrahydrozoline Hydrochloride in Ophthalmic Solution

AlAani

Alnukkary

2016

Adv Pharm Bull

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Development and Validation of an HPLC Method for the Determination of Dexamethasone, Dexamethasone Sodium Phosphate and Chloramphenicol in Presence of Each Other in Pharmaceutical Preparations

Cited by 30 publications

References 1 publication

Stability Indicating Rp-HPLC Method for Simultaneous Determination of Ciprofloxacin and Dexamethasone in Binary Combination

Stability Indicating Rp-HPLC Method for Simultaneous Determination of Ciprofloxacin and Dexamethasone in Binary Combination

Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination

Stability-Indicating HPLC Method for Simultaneous Determination of Chloramphenicol, Dexamethasone Sodium Phosphate and Tetrahydrozoline Hydrochloride in Ophthalmic Solution

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