Stability-Indicating HPLC Method for Simultaneous Determination of Chloramphenicol, Dexamethasone Sodium Phosphate and Tetrahydrozoline Hydrochloride in Ophthalmic Solution

AlAani, Hashem; Alnukkary, Yasmin

doi:10.15171/apb.2016.020

Cited by 15 publications

(13 citation statements)

References 4 publications

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“…Each formulation was developed and assessed in triplicate. Drug release of dexamethasone phosphate and ketorolac tris salt was quantified by HPLC as previously described by H. AlAani et al with some modifications [28]. The study was performed with a Waters Alliance 2695 separation module equipped with Waters photodiode array 2996 (Waters corp, Barcelona, Spain).…”

Section: In Vitro Release Studiesmentioning

confidence: 99%

Thermo-Responsive PLGA-PEG-PLGA Hydrogels as Novel Injectable Platforms for Neuroprotective Combined Therapies in the Treatment of Retinal Degenerative Diseases

et al. 2021

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The present study aims to develop a thermo-responsive-injectable hydrogel (HyG) based on PLGA-PEG-PLGA (PLGA = poly-(DL-lactic acid co-glycolic acid); PEG = polyethylene glycol) to deliver neuroprotective agents to the retina over time. Two PLGA-PEG PLGA copolymers with different PEG:LA:GA ratios (1:1.54:23.1 and 1:2.25:22.5) for HyG-1 and HyG-2 development respectively were synthetized and characterized by different techniques (gel permeation chromatography (GPC), nuclear magnetic resonance (NMR), dynamic light scattering (DLS), critical micelle concentration (CMC), gelation and rheological behaviour). According to the physicochemical characterization, HyG-1 was selected for further studies and loaded with anti-inflammatory drugs: dexamethasone (0.2%), and ketorolac (0.5%), alone or in combination with the antioxidants idebenone (1 µM) and D-α-Tocopherol polyethylene glycol 1000 succinate (TPGS) (0.002%). In vitro drug release and cytotoxicity studies were performed for the active substances and hydrogels (loaded and drug-free). A cellular model based on oxidative stress was optimized for anti-inflammatory and antioxidant screening of the formulations by using retinal-pigmented epithelial cell line hTERT (RPE-1). The copolymer 1, used to prepare thermo-responsive HyG-1, showed low polydispersity (PDI = 1.22) and a strong gel behaviour at 25% (w/v) in an isotonic buffer solution close to the vitreous temperature (31–34 °C). Sustained release of dexamethasone and ketorolac was achieved between 47 and 62 days, depending on the composition. HyG-1 was well tolerated (84.5 ± 3.2%) in retinal cells, with values near 100% when the anti-inflammatory and antioxidant agents were included. The combination of idebenone and dexamethasone promoted high oxidative protection in the cells exposed to H2O2, with viability values of 86.2 ± 14.7%. Ketorolac and dexamethasone-based formulations ameliorated the production of TNF-α, showing significant results (p ≤ 0.0001). The hydrogels developed in the present study entail a novel biodegradable tool to treat neurodegenerative processes of the retina overtime.

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Section: In Vitro Release Studiesmentioning

confidence: 99%

Thermo-Responsive PLGA-PEG-PLGA Hydrogels as Novel Injectable Platforms for Neuroprotective Combined Therapies in the Treatment of Retinal Degenerative Diseases

et al. 2021

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“…Za opisivanje reakcija koristi se zakon brzine reakcije, koji može imati diferencijalni i integralni oblik što je opisano u literaturi [14,30], a način određivanja reda reakcije dat je u radovima [31,32]. Kao konačan podatak izračunava se konstanta brzine reakcije [33][34][35].…”

Section: Rezultati I Diskusijaunclassified

Investigation of olopatadine hydrochloride under stress conditions by hydrophilic interaction liquid chromatography

Maksić

Tumpa²,

Popović

et al. 2016

Hem Ind

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IzvodU savremenoj farmaceutskoj analizi neke farmaceutski aktivne supstance studije forsirane degradacije imaju veliki značaj jer se iz njih može dobiti veliki broj značajnih posataka o stabilnosti leka. U ovom radu, opisano je ispitivane stabilnosti olopatadin-hidrohlorida pod utcajem stres agenasa a u cilju dobijanja podataka o njegovoj stabilnosti. Za praćenje ponašanja olopatadin-hidrohlorida primenjena je metoda tečne hromatografije hidrofilnih interakcija (eng. Hidrophilic Interaction Liquid Chromatography -HILIC). Olopatadin-hidrohlorid izložen je dejstvu kiseline, baze, povišene temperature i oksidacionog sredstva, a stepen degradacije praćen je u definisanim vremenskim intervalima. Takođe, određena je i kinetika degradacije u cilju sticanja boljeg uvida u stabilnost supstance i njen mehanizam razgradnje. Definisani su red reakcije, konstanta brzine reakcije i poluvreme degradacije nakon izlaganja dejstvu kiseline, baze i oksidacionog sredstva.Ključne reči: olopatadin-hidrohlorid; studije forsirane degradacije; kinetika degradacije; tečna hromatografija hidrofilnih interakcija.

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“…Reviewing the literature, various spectrophotometric methods were used to evaluate this mixture in its pharmaceutical dosage form [17,18]. Three HPLC chromatographic methods for evaluating this mixture have also been reported [19][20][21]. Also a voltammetric method was mentioned [22].…”

Section: Introductionmentioning

confidence: 99%

“…According to this, the present work introduces first, green, selective and sensitive TLC-densitometric methods which are time-saving for the determination of ophthalmic ternary mixture either in absence or presence of PNA by using GAC. The developed TLC methods have been compared to the reported HPLC method regarding eco-scaling for green assessment [21]. Eco-scaling of TLC methods was calculated depending on penalty points (based on reagents and instruments) and subtracted from a base of 100 (the score of an ideal green analytical method) [23].…”

Section: Introductionmentioning

confidence: 99%

Smart TLC–densitometric methods for determination of ophthalmic ternary mixture containing chloramphenicol in the presence of its synthetic precursor: Comparative eco-scaling for greenness assessment

Eissa

El-Hadi

Zaazaa

et al. 2020

JPC-J Planar Chromat

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Green analytical methods have gained a growing interest in the field of pharmaceutical research to reduce impacts on the environment and enhance analysts' health safety. Chloramphenicol (CHL), dexamethasone sodium phosphate (DSP) and tetrahydrozoline HCl (THZ) form an ophthalmic ternary mixture that is co-formulated for conjunctivitis treatment. In the present work, for time saving and higher sensitivity, two green thin-layer chromatography (TLC) methods were developed for the determination of this ophthalmic ternary mixture in the absence or presence of p-nitroacetophenone (PNA), a synthetic precursor of chloramphenicol. In both proposed methods, silica gel 60 F 254 plates were used as the stationary phase. The mobile phase used for method (A) was ethanol-water-ammonia (7.0:2.5:0.5, V/V), while, for method (B), acetonitrilewater-ammonia (10.0:3.0:0.5, V/V) was used as the mobile phase. TLC separation was followed by quantitative determination of the aforementioned drugs at wavelengths 242.0 nm and 220.0 nm. Both methods were validated in compliance with the International Conference on Harmonisation (ICH) guidelines, where both methods were found to be reliable, reproducible, and selective. Statistical comparison of the developed methods was done with a reported high-performance liquid chromatography (HPLC) method where no significant difference was found. Analytical eco-scaling depends on penalty point which was calculated to be 92, 88 and 87 for methods A, B and the reported HPLC, respectively, suggesting that the proposed methods are eco-friendlier with penalty point scoring very high on the scale than the reported one.

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Stability-Indicating HPLC Method for Simultaneous Determination of Chloramphenicol, Dexamethasone Sodium Phosphate and Tetrahydrozoline Hydrochloride in Ophthalmic Solution

Cited by 15 publications

References 4 publications

Thermo-Responsive PLGA-PEG-PLGA Hydrogels as Novel Injectable Platforms for Neuroprotective Combined Therapies in the Treatment of Retinal Degenerative Diseases

Thermo-Responsive PLGA-PEG-PLGA Hydrogels as Novel Injectable Platforms for Neuroprotective Combined Therapies in the Treatment of Retinal Degenerative Diseases

Investigation of olopatadine hydrochloride under stress conditions by hydrophilic interaction liquid chromatography

Smart TLC–densitometric methods for determination of ophthalmic ternary mixture containing chloramphenicol in the presence of its synthetic precursor: Comparative eco-scaling for greenness assessment

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