Development and validation of an LC-MS/MS method for simultaneous quantification of hesperidin and hesperetin in rat plasma for pharmacokinetic studies

Lee, Jr Ting; Pao, Li-Heng; Hsieh, Chang Da; Huang, Pei Wei; Hu, Oliver Yoa Pu

doi:10.1039/c7ay00051k

Cited by 15 publications

(11 citation statements)

References 36 publications

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“…The six metabolites were identified and included flavonoids known with their excellent antioxidant activities owned to the presence of ortho-dihydroxyl groups and their role in the structure-activity relationship [ 89 ]. The flavonoid 7,3’,4’-trihydroxyisoflavone (3′-hydroxydaidzein) was mentioned before to inhibit the lipid peroxidation in the rat liver with IC50 4.1 µM [ 54 ]. It exerted also the same1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity as α-tocopherol and similar antimutagenic activity to 6-hydroxydaidzein [ 90 ].…”

Section: Discussionmentioning

confidence: 99%

The Ameliorative Role of Acacia senegal Gum against the Oxidative Stress and Genotoxicity Induced by the Radiographic Contrast Medium (Ioxitalamate) in Albino Rats

et al. 2021

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Arabic gum (Acacia senegal, AG) is proven effective antioxidant and cytoprotective agent. The present study was designed to test this notion by investigating the possible role of AG against the radiographic contrast medium (Ioxitalamate, Telebrix-35®, TBX)-induced oxidative stress and genotoxicity. Albino rats were divided into four groups and supplied with either; distilled water, daily 10% (w/v) AG, an intravenous dose of TBX (1600 mg I/kg b.wt) and co-administration of TBX and AG. Rats were sacrificed and blood samples were collected to assess the genotoxicity employing the peripheral blood leucocytes fluorescent double staining; namely the acridine orange/ethidium bromide (AO/EB) staining and alkaline comet assay. Further, chromosomal analyses were done in bone marrow cells. Serum urea and creatinine levels, in addition to malondialdehyde (MDA), nitric oxide (NO), catalase (CAT) and glutathione (GSH) levels in kidney tissues were measured. Liquid chromatography-mass spectrophotometry (LC-MS-MS) was performed to identify the chemical composition of AG extract. Kidney functions, single/double-stranded DNA damage, chromosomal aberrations, mitotic index, MDA and NO levels were significantly (p < 0.001) increased in TBX-treated group compared to the control and AG-treated one. Meanwhile, CAT and GSH activities were significantly diminished and the AG supplementation significantly (p < 0.001) ameliorated these effects compared with the control and AG-treated groups. Five compounds have been identified using GNPS networking including 7,3′,4′-Trihydroxyisoflavone, Noscapine, Tetrahydropapaveroline, Costunolide, Hesperidin. In conclusion, results of the present study suggest that AG exerted a protective role against TBX-induced oxidative stress and genotoxicity which may be attributed to the active metabolites in the gum.

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Section: Discussionmentioning

confidence: 99%

The Ameliorative Role of Acacia senegal Gum against the Oxidative Stress and Genotoxicity Induced by the Radiographic Contrast Medium (Ioxitalamate) in Albino Rats

et al. 2021

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“…Then, there was a second peak. 42 By comparing the results of their pharmacokinetic studies, we found that eriocitrin, naringin and hesperidin were rapidly absorbed aer oral administration, and all of them showed double peaks, which may be related to their structures; however, further investigation is needed.…”

Section: Pharmacokinetic Studymentioning

confidence: 94%

The development and validation of an HPLC-MS/MS method for the determination of eriocitrin in rat plasma and its application to a pharmacokinetic study

Feng

Xue

et al. 2020

RSC Adv.

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“…The hesperidin content in samples was identified and quantified using the multiple reaction monitoring mode with the following transition: hesperidin m/z 610.97 → m/z 302.95. 26 The relative content of hesperidin was determined based on the analytical area of sample peaks using an automated peak annotation program in the Waters MassLynx4.1 software (Milford, MA).…”

Section: Quantification Of Hesperidin Content In the Fermented Tea Samplementioning

confidence: 99%

Rapid LC-MS/MS Determination of Hesperidin in Fermented Tea Prepared from Unripe Satsuma Mandarin (Citrus unshiu) Fruits and Third-crop Green Tea (Camellia sinensis) Leaves

et al. 2020

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For improving quality control in the fermented tea production process and advancing the corresponding food labeling with function claims, a rapid and robust hesperidin analysis method using LC-MS/MS with the sample dilution approach was developed by following internationally accepted criteria of the Association of Official Analytical Chemists (AOAC). The linear correlation coefficient (r 2 ) of the regression line was 0.9997 in the concentration range of 0.025 -2.5 mg/L. The matrix effect evaluated using regression line slope values was negligible. The recovery rate of 100.7% indicated improved trueness. The performance of the newly developed method in determining the hesperidin content of fermented tea samples did not significantly vary from that of a well-established, conventional method. The HorRat values of intra-and inter-laboratory reproducibility studies were both within the acceptable range, indicating sufficient accuracy of the newly developed method according to the AOAC criteria.

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Development and validation of an LC-MS/MS method for simultaneous quantification of hesperidin and hesperetin in rat plasma for pharmacokinetic studies

Abstract: A simple and sensitive method for the determination of hesperidin and hesperetin was necessary for a pharmacokinetic (PK) study in Wistar rats.

Cited by 15 publications

References 36 publications

The Ameliorative Role of Acacia senegal Gum against the Oxidative Stress and Genotoxicity Induced by the Radiographic Contrast Medium (Ioxitalamate) in Albino Rats

The Ameliorative Role of Acacia senegal Gum against the Oxidative Stress and Genotoxicity Induced by the Radiographic Contrast Medium (Ioxitalamate) in Albino Rats

The development and validation of an HPLC-MS/MS method for the determination of eriocitrin in rat plasma and its application to a pharmacokinetic study

Rapid LC-MS/MS Determination of Hesperidin in Fermented Tea Prepared from Unripe Satsuma Mandarin (Citrus unshiu) Fruits and Third-crop Green Tea (Camellia sinensis) Leaves

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