The development and validation of an HPLC-MS/MS method for the determination of eriocitrin in rat plasma and its application to a pharmacokinetic study

Li, Luya; Feng, Rui; Xue, Feng; Chen, Yuting; Liu, Xin; Sun, Wenjing; Zhang, Lantong

doi:10.1039/c9ra10925k

Cited by 7 publications

(4 citation statements)

References 43 publications

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“…A peak demonstrated an [M-H]- m/z at 333 and daughter ions at m/z 253 [M-H-SO 3 ]-, 225 [M-H-SO 3 -CO]-, 224 [M-H-SO3-CHO]-, 209 [M-H-SO 3 -CO 2 ]-, 197 [M-H-SO 3 -2CO]-, 185 [M-H-SO3-2CO + C]-, and 135 [M-H-SO 3 -C 8 H 6 O]; it was assigned as daidzein 4′-sulfate ( Zhao et al, 2018 ). A peak demonstrated an [M-H]- m/z at 595 and daughter ions at m/z 433 [M-H-Glc]-, 287 [M-H-Glc-Rha]-; it was assigned as eriocitrin ( Li et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

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Bio-fabricated zinc oxide nanoparticles mediated by endophytic fungus Aspergillus sp. SA17 with antimicrobial and anticancer activities: in vitro supported by in silico studies

Abdelrahman,

El Hawary,

Mohsen

et al. 2024

Front. Microbiol.

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IntroductionIn recent years, the world’s attention has been drawn to antimicrobial resistance (AMR) because to the frightening prospect of growing death rates. Nanomaterials are being investigated due to their potential in a wide range of technical and biological applications.MethodsThe purpose of this study was to biosynthesis zinc oxide nanoparticles (ZnONPs) using Aspergillus sp. SA17 fungal extract, followed by characterization of the produced nanoparticles (NP) using electron microscopy (TEM and SEM), UV-analysis, X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FT-IR).Results and DiscussionThe HR-TEM revealed spherical nanoparticles with an average size of 7.2 nm, and XRD validated the crystalline nature and crystal structure features of the generated ZnONPs, while the zeta potential was 18.16 mV, indicating that the particles’ surfaces are positively charged. The FT-IR was also used to identify the biomolecules involved in the synthesis of ZnONPs. The antibacterial and anticancer properties of both the crude fungal extract and its nano-form against several microbial strains and cancer cell lines were also investigated. Inhibition zone diameters against pathogenic bacteria ranged from 3 to 13 mm, while IC50 values against cancer cell lines ranged from 17.65 to 84.55 M. Additionally, 33 compounds, including flavonoids, phenolic acids, coumarins, organic acids, anthraquinones, and lignans, were discovered through chemical profiling of the extract using UPLC-QTOF-MS/MS. Some molecules, such pomiferin and glabrol, may be useful for antibacterial purposes, according to in silico study, while daidzein 4’-sulfate showed promise as an anti-cancer metabolite.

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Section: Resultsmentioning

confidence: 99%

“…; it was assigned as daidzein 4′-sulfate (Zhao et al, 2018). A peak demonstrated an [M-H]-m/z at 595 and daughter ions at m/z 433 [M-H-Glc]-, 287 [M-H-Glc-Rha]-; it was assigned as eriocitrin (Li et al, 2020).…”

Section: Phenolic Organic and Fatty Acids And Their Derivativesmentioning

confidence: 99%

Bio-fabricated zinc oxide nanoparticles mediated by endophytic fungus Aspergillus sp. SA17 with antimicrobial and anticancer activities: in vitro supported by in silico studies

Abdelrahman,

El Hawary,

Mohsen

et al. 2024

Front. Microbiol.

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“…In this work, chromatographic and mass parameters were extensively optimized for the analytes and internal standard determination. To the best of our knowledge, chromatographic conditions, particularly mobile phase comparison, played a critical role in developing good chromatographic behavior, which includes peak symmetry sensitivity, short run duration, and suitable ionization [27][28][29].…”

Section: Chromatography and Tandem Mass Spectrometrymentioning

confidence: 99%

Lc-MS/MS-Based Quantitative Profiling of Papain Enzyme in Carica Papaya L.: Method Development and Validation

C.,

AGALAVE,

TYAGI

et al. 2024

Int J App Pharm

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Objective: To develop and validate a sensitive Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) method for quantifying the Papain enzyme in Carica papaya L. plant extracts. Methods: As an internal standard (IS), Papain was procured. A gradient-based LC-MS/MS method was developed using the Quasar C18, 150 mm × 2.1 mm, 3 µm column; the drug separation was accomplished using a gradient elution mode with a mobile phase consisting of 0.2% formic acid in water (A) and a 98:2% ratio of formic acid in acetonitrile (B). The mobile phase was injected with a volume of 10 µl at a flow rate of 0.5 ml/min. Results: Matrix-matched calibration adhering to SANCO guidelines was employed for accurate quantification, covering a concentration range of 1 to 2000 ng/ml. The method's selectivity and linearity were confirmed with an R2 correlation value of 0.990. Limits of detection (LOD) and quantification (LOQ) were determined as 170.5 ng/ml and 516.8 ng/ml, respectively. The developed LC-MS/MS method demonstrated robust analytical performance, providing a solid basis for quantifying Papain enzyme levels in Carica papaya L. plant extracts. The matrix-matched calibration approach, with a well-defined standard calibration curve, showcased the method's selectivity and linearity. The LOD and LOQ values further underscored the method's sensitivity. Conclusion: This study successfully establishes and validates a gradient-based LC-MS/MS method for accurate quantification of Papain enzyme levels in Carica papaya L. plant extracts. The demonstrated precision, accuracy, and sensitivity support the reliability of this analytical approach, offering a valuable tool for future investigations into the biological effects of Papain.

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“…Aer being vortexed for 3 min, the mixture was subjected to a 21,380g centrifugation for 10 min at 4 C. The collected supernatant was evaporated by nitrogen gas and redissolved into 100 ml of methano l. The sample was puried by twice centrifugation (21,380g) for 10 min, and a 10 ml of the supernatant was loaded on the LC-MS/MS for analysis. 22…”

Section: Sample Preparationmentioning

confidence: 99%

Assessment of a developed HPLC-MS/MS approach for determining plasma eupatorin in rats and its application in pharmacokinetics analysis

Feng

Zhang

et al. 2020

RSC Adv.

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The development and validation of an HPLC-MS/MS method for the determination of eriocitrin in rat plasma and its application to a pharmacokinetic study

Abstract: Eriocitrin is one of the major active constituents of lemon fruit, and it possesses strong antioxidant, lipid-lowering, anticancer and anti-inflammatory activities and has long been used in food, beverages and wine.

Cited by 7 publications

References 43 publications

Bio-fabricated zinc oxide nanoparticles mediated by endophytic fungus Aspergillus sp. SA17 with antimicrobial and anticancer activities: in vitro supported by in silico studies

Bio-fabricated zinc oxide nanoparticles mediated by endophytic fungus Aspergillus sp. SA17 with antimicrobial and anticancer activities: in vitro supported by in silico studies

Lc-MS/MS-Based Quantitative Profiling of Papain Enzyme in Carica Papaya L.: Method Development and Validation

Assessment of a developed HPLC-MS/MS approach for determining plasma eupatorin in rats and its application in pharmacokinetics analysis

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