Rapid LC-MS/MS Determination of Hesperidin in Fermented Tea Prepared from Unripe Satsuma Mandarin (Citrus unshiu) Fruits and Third-crop Green Tea (Camellia sinensis) Leaves

Miura, Masashi; Nogami, Mamiko; Sakai, Miho; Sato, Miwa; Yatsushiro, Takeshi

doi:10.2116/analsci.20p100

Cited by 10 publications

(4 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hesperidin and vitex were analyzed as described previously with some modifications (Miura et al, 2020). A 200 mg sample of powder was weighed and extracted overnight at 4°C in 1.0 ml of 70% aqueous methanol.…”

Section: Methodsmentioning

confidence: 99%

Microbial biotransformation of Pericarpium Citri Reticulatae (PCR) by Aspergillus niger and effects on antioxidant activity

Wang

Chen

et al. 2020

Food Science & Nutrition

View full text Add to dashboard Cite

Pericarpium Citri Reticulatae (PCR), the mature fruit peel of Citrus reticulata Blanco and its different cultivars, is an important citrus by‐product with beneficial health and nutritive properties. However, due to the lack of value‐added methods for its development and utilization, a large amount of PCR is discarded or wasted. To explore a possibly more effective method to utilize PCR, we compared the chemical and biological differences before (CK) and after (CP) microbial transformation of PCR by Aspergillus niger. UPLC‐ESI‐MS/MS, HPLC, and LC‐MS methods were used to compare the chemical profiles of CK and CP. The results demonstrated that microbial biotransformation by A. niger could transform flavonoid compounds by utilizing the carbohydrate and amino acid nutrients in PCR. This could also promote the accumulation of polyhydroxyflavones compounds in CP. The antioxidant assay demonstrated that CP had significantly greater free radical‐scavenging activity than CK. The higher antioxidant activity of CP may result from the high level of flavonoids with associated phenolic hydroxyl groups. Microbial biotransformation is an effective method for improving the antioxidant capacity of PCR and may be effective and useful in other natural product situations.

show abstract

Section: Methodsmentioning

confidence: 99%

Microbial biotransformation of Pericarpium Citri Reticulatae (PCR) by Aspergillus niger and effects on antioxidant activity

Wang

Chen

et al. 2020

Food Science & Nutrition

View full text Add to dashboard Cite

show abstract

“…In the flavedo, the treatments led to irregular outcomes, with the compound decreasing in 'Tarocco' pectinase-treated. Hesperidin is a flavanone glycoside that is composed by the aglycone hesperetin attached to a rutinose disaccharide [26]. Since in 'Tarocco' flavedo the reduction in hesperidin was offset by an increase in hesperetin, it is very likely that the enzyme pectinase performed an efficient digestion of the tissue, which led to the hydrolysis of the flavanone glycoside with consequent release of the aglycones moiety.…”

Section: Enzyme-assisted Extraction Of Phenolic Compoundsmentioning

confidence: 99%

Flavedo and albedo of five citrus fruits from Southern Italy: physicochemical characteristics and enzyme-assisted extraction of phenolic compounds

et al. 2021

View full text Add to dashboard Cite

Peels are major byproducts of the juice processing industry. Citrus peels are composed of two tissues, namely the flavedo (outer layer) and the albedo (inner layer). Here, flavedo and albedo were obtained from the peel of grapefruit cv. 'Star ruby', lemon cv. 'Akragas', and sweet orange cvs. 'Tarocco', 'Sanguinello' and 'Moro'. Freeze dried flavedo and albedo were investigated for their functional properties, and subjected to enzyme-assisted extraction with pectinase and cellulase (concentration of 6 U g −1 DW) to extract the phenolic compounds. Albedo showed superior physicochemical characteristics than flavedo. Albedo from 'Tarocco' exhibited the maximum water holding capacity (10.9 ± 0.48 g/g). Grapefruit albedo revealed the highest water swelling (13.4 ± 2.62 mL/g) and oil binding (6.31 ± 2.62 g/g) capacities. As regards the enzyme-assisted extraction of phytochemicals, both enzymes enhanced the extraction of phenolics (p < 0.05) compared to the controls. In flavedo, pectinase and cellulase increased the recovery of phenolics by approx. 30%, whereas in albedo the yields were increased above 60%. Results from this investigation suggest that albedo might be used by the food industry as a novel raw material, due to its superior physicochemical properties. Moreover, results provide evidence that the enzyme-assisted extraction is advantageous to recover phenolics from the citrus peels.

show abstract

“…Efficient and accurate analytical methods for rapid and sensitive detection of hesperidin content in such an agricultural product, therefore, continue to be a topic of interest. Although separation-based analytical methods such as chromatography enable highly sensitive and selective hesperidin detection [ 8 , 9 , 10 ], these methods are often time-consuming and require complex procedures and costly equipment.…”

Section: Introductionmentioning

confidence: 99%

Portable Microelectrochemical Sensors for Rapid and Sensitive Determination of Hesperidin in Citrus reticulate ‘Chachi’ Peel

et al. 2023

View full text Add to dashboard Cite

Portable and low-cost analytical devices are essential for rapid detection of bioactive substrates in agricultural products. This study presents the first highly integrated microelectrochemical sensor based on pencil graphite for rapid and sensitive detection of hesperidin in Citrus reticulate ‘Chachi’ peel. The surface morphology and characterization as well as the electrochemical property of pencil graphite was investigated and discussed. A high electrocatalytic efficiency of hesperidin has been found at used pencil graphite-based microelectrodes. Kinetic analysis was carried out to further understand the electrochemical process of hesperidin at a pencil graphite microelectrode. Consequently, a portable and highly-integrated microelectrochemical sensor exhibits a sensitivity of 0.7251 μA cm−2 μM−1 and a detection limit as low as 25 nM (S/N = 3), and high selectivity was fabricated. Proposed microelectrochemical sensors were applied to electrochemically determinate the hesperidin content in the extract of Citrus reticulata “chachi” peel. As a result, the concentration of hesperidin in the actual real sample detected electrochemically with the proposed portable and low-cost microelectrochemical sensors is highly consistent to that obtained with a common chromatographic method, thus indicating the good reliability and that it can be used in practical applications.

show abstract

Rapid LC-MS/MS Determination of Hesperidin in Fermented Tea Prepared from Unripe Satsuma Mandarin (Citrus unshiu) Fruits and Third-crop Green Tea (Camellia sinensis) Leaves

Cited by 10 publications

References 28 publications

Microbial biotransformation of Pericarpium Citri Reticulatae (PCR) by Aspergillus niger and effects on antioxidant activity

Microbial biotransformation of Pericarpium Citri Reticulatae (PCR) by Aspergillus niger and effects on antioxidant activity

Flavedo and albedo of five citrus fruits from Southern Italy: physicochemical characteristics and enzyme-assisted extraction of phenolic compounds

Portable Microelectrochemical Sensors for Rapid and Sensitive Determination of Hesperidin in Citrus reticulate ‘Chachi’ Peel

Contact Info

Product

Resources

About