Development and Validation of a New Low-Cost Enzyme-Linked Immunoassay for Serum and Dried Blood Spot Thyroglobulin

Stinca, Sara; Andersson, Maria; Erhardt, Juergen; Zimmermann, Michael

doi:10.1089/thy.2015.0428

Cited by 33 publications

(24 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More studies using different assays in various populations are needed to clarify the usefulness of a specific cut‐off value for median Tg to indicate ID in a population. An interesting approach when using Tg for monitoring is the use of dried blood spot for analyses …”

Section: Discussionmentioning

confidence: 99%

Serum thyroglobulin as a biomarker of iodine deficiency in adult populations

Krejbjerg

Bjergved

Pedersen

et al. 2016

Clinical Endocrinology

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Serum thyroglobulin as a biomarker of iodine deficiency in adult populations

Krejbjerg

Bjergved

Pedersen

et al. 2016

Clinical Endocrinology

View full text Add to dashboard Cite

show abstract

“…For transport shorter than 8 h, they were transported in room temperature, which does not influence their quality. DBS-Tg was measured with a DBS-Tg sandwich enzyme-linked immunosorbent assay at ETH Zurich (Zurich, Switzerland) [24]. Serum control samples (Liquicheck Tumor Marker Control, LOT.19970 and LOT.19980; Bio-Rad, Hercules, CA, USA) were used as standards for the DBS-Tg assay.…”

Section: Thyroglobulin Concentrationmentioning

confidence: 99%

Iodine deficiency in pregnant women in Sweden: a national cross-sectional study

et al. 2019

Self Cite

View full text Add to dashboard Cite

Purpose Voluntary salt iodization at 50 mg/kg salt ensures adequate iodine nutrition in Swedish school-aged children, but iodine status in pregnant women is uncertain. Methods We conducted a cross-sectional national study of 743 pregnant women, at median gestational age of 23 weeks (IQR 9, 38), recruited from maternal health care centers. We measured: urinary iodine concentration (UIC) and urinary creatinine concentration in spot urine samples; thyroglobulin (Tg), thyroid-stimulating hormone (TSH), and total thyroxine (tT4) on dried blood spots (DBS); and thyreoperoxidase antibodies in serum samples. Data on dietary supplement use were obtained, and women were classified as supplement users (consuming multivitamins containing ≥ 150 µg iodine/day) and non-supplement users (no supplements or < 150 µg iodine/day from supplements). Results Overall median UIC [bootstrapped 95% confidence interval (CI)] was 101 µg/L (95, 108; n = 737): 149 µg/L (132, 164) in supplement users (n = 253) and 85 µg/L (79, 92) in non-supplement users (n = 440) (p < 0.001). Overall geometric mean DBS-Tg (95% CI) was 22.1 μg/L (20.8, 23.5; n = 675) and the prevalence of elevated DBS-Tg was 19%. DBS-Tg was lower in supplement users (n = 229) than in non-supplement users (n = 405) (19.1 vs 24.4 μg/L, p < 0.001). DBS-TSH, DBS-tT4, and S-TPOab positivity did not differ between the two groups. Conclusions Pregnant women in Sweden have inadequate iodine nutrition. Women not taking iodine supplements containing ≥ 150 µg iodine/day are affected by mild iodine deficiency and are at higher risk for increased thyroid activity, while maintaining euthyroidism. Iodine intake should be improved in women both before and after conception by promotion of iodized salt instead of non-iodized salt. We urge regular monitoring of iodine status in the general Swedish population, as well as in risk groups.

show abstract

“…Thyroid function. DBS-Tg was measured at the Human Nutrition Laboratory of the ETH Zurich using a DBS-Tg enzyme-linked immunosorbent assay, as described elsewhere (44). Serum control samples (Liquicheck Tumor Marker Control, LOT.23911, 23912, 23913; Bio-Rad, Hercules, CA) were used as standards for the DBS Tg assays.…”

Section: Laboratory Analysesmentioning

confidence: 99%

Effect of Excess Iodine Intake from Iodized Salt and/or Groundwater Iodine on Thyroid Function in Nonpregnant and Pregnant Women, Infants, and Children: A Multicenter Study in East Africa

Farebrother

Zimmermann

Abdallah

et al. 2018

Thyroid

Self Cite

View full text Add to dashboard Cite

Although iodine intake was excessive and Tg concentrations were elevated, there was little impact on thyroid function. Chronic excess iodine intake thus appears to be well tolerated by women, infants, and children. However, such high iodine intake is unnecessary and should be avoided. Careful evaluation of contributions from both iodized salt and groundwater iodine is recommended before any review of iodization policy is considered.

show abstract

Development and Validation of a New Low-Cost Enzyme-Linked Immunoassay for Serum and Dried Blood Spot Thyroglobulin

Abstract: These new low-cost serum and DBS-Tg assays perform well over a wide range of Tg concentrations, and the field-friendly DBS assay may be particularly useful in population studies of iodine nutrition.

Cited by 33 publications

References 31 publications

Serum thyroglobulin as a biomarker of iodine deficiency in adult populations

Serum thyroglobulin as a biomarker of iodine deficiency in adult populations

Iodine deficiency in pregnant women in Sweden: a national cross-sectional study

Effect of Excess Iodine Intake from Iodized Salt and/or Groundwater Iodine on Thyroid Function in Nonpregnant and Pregnant Women, Infants, and Children: A Multicenter Study in East Africa

Contact Info

Product

Resources

About