Development and validation of a sensitive LC–MS/MS method for the simultaneous quantitation of theophylline and its metabolites in rat plasma

Chae, Jung-Woo; Kim, Dong-Hyun; Lee, Byung‐yo; Kim, Eun jung; Kwon, Kwang‐il

doi:10.1016/j.jchromb.2012.01.028

Cited by 14 publications

(6 citation statements)

References 16 publications

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“…The mean integrated peak areas of the LQC (lowest quality control) and HQC (highest quality control) samples were compared before and after the stability testing described in the experimental section. No stability issues were observed from any of these experiments (Chae et al, 2012).…”

Section: Analytical Methods Validationmentioning

confidence: 79%

“…The following precursors to product ion transitions were used in the SRM of theophylline, 1-MX, 3-MX, 1,3-DMU, 1-MU, and the IS, respectively, m/z 179-164, m/z 165-108, m/z 165-122, m/z 195-110, m/z 181-138, and m/z 168-132 with dwell times of 250 ms. The mass spectrometer was operated at unit mass resolution for both the first and third quadrupoles (Chae et al, 2012).…”

Section: Liquid Chromatography/mass Spectrometrymentioning

confidence: 99%

See 1 more Smart Citation

Effect of decursin on the pharmacokinetics of theophylline and its metabolites in rats

Chae¹,

Baek²,

Kwon³

2012

Journal of Ethnopharmacology

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Section: Analytical Methods Validationmentioning

confidence: 79%

Section: Liquid Chromatography/mass Spectrometrymentioning

confidence: 99%

Effect of decursin on the pharmacokinetics of theophylline and its metabolites in rats

Chae¹,

Baek²,

Kwon³

2012

Journal of Ethnopharmacology

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“…The use of MS has decreased detection limits to ng/mL and using ultra-high performance liquid chromatography (UHPLC) has led to a significant reduction of run times (under 10 minutes). However, many of the published methods for theophylline determination involve multistep extractions, large volumes of organic solvent, and require a complicated sample preparation (17,18). A hydrophilic interaction liquid chromatography (HILIC) separation mode has emerged as a valuable complementary technique to reversed-phase (RP), being especially suitable for an analysis of polar and ionized solutes which are difficult to retain in RP.…”

Section: A C T a M E D I C A M A R T I N Imentioning

confidence: 99%

Quantification of Theophylline in Guinea Pig Plasma by LC-MS/MS Using Hydrophilic Interaction Liquid Chromatography Stationary Phase: Method Development, Validation, and Application in Study

Kertys

Urbanová

Mokrý

2017

Acta Medica Martiniana

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Theophylline has been used in the treatment of bronchial asthma and chronic obstructive pulmonary disease (COPD) for over 70 years. In order to maximize the effectiveness and safety of theophylline therapy it is important to individualize the dosage of the drug. In our study we focused on determination of theophylline concentrations in guinea pig plasma. A rapid, specific, and reliable LC-MS/MS-based method was developed and validated according to European Medicine Agency (EMA) guidelines. A hydrophilic interaction liquid chromatography (HILIC) separation mode for reduction time of sample preparation was used. The analysed sample was quantified in a positive ionization mode. Multiple reaction monitoring (MRM) using transition m/z 181.06→124.06 and m/z 187.17→127.06 was performed to quantify theophylline with deuterated internal standard ([2H6]-theophylline), respectively. Modification of collision energies was performed in parallel with chromatographic separation to further eliminate interference from the matrix. The method was validated for a range of 0.5 to 30 μg/mL of plasma sample. The intra-day and inter-day precision and accuracy of the quality control samples at low, me dium, and high concentration levels exhibited relative standard deviations (RSD) of less than 10 %. The method was successfully applied for the quantitation of theophylline in guinea pig plasma for better understanding its effects in a model of ovalbumin-induced allergic inflammation.

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“…[21][22][23][24] However, this technique was applicable to urine and blood samples and not measurable for all demethylated metabolites in environmental samples (i.e., wastewater and river water). For biodegradation of organic compounds, such as nonylphenol, structural isomers of the metabolites were expected to occur in wastewater samples as the treatments involve complicated degradation processes.…”

Section: Introductionmentioning

confidence: 99%

Determination of Caffeine and Its Metabolites in Wastewater Treatment Plants Using Solid-Phase Extraction and Liquid Chromatography–Tandem Mass Spectrometry

Echigo

Itoh

2018

ANAL. SCI.

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For caffeine and its seven major metabolites (i.e., theobromine, theophylline, paraxanthine, 1-methylxanthine, 3-methylxanthine, 7-methylxanthine, and xanthine), an optimized analytical method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for their detection in wastewater samples was developed in this study. Extraction of these compounds (recoveries ranged from 60.3 to 83.2%) was made possible by combining universal polymeric reversedphase cartridge and polymeric strong cation exchange cartridge. This method was applied to the determination of caffeine and its metabolites in the influent and effluent of an anaerobic-anoxic-oxic (A2O) process. In the A2O influent, caffeine and its metabolites (except xanthine) ranged from 1.39 to 5.45 μg/L, and their concentrations in the A2O effluent ranged from 10.2 to 171.3 ng/L. The mass load of caffeine was 14.9 g/day/1000 inhabitants, considering the population served by the target wastewater treatment plant (WWTP). The concentration of caffeine derivatives in wastewater influent is a tool for estimating the population size in the area served by WWTPs.

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Development and validation of a sensitive LC–MS/MS method for the simultaneous quantitation of theophylline and its metabolites in rat plasma

Cited by 14 publications

References 16 publications

Effect of decursin on the pharmacokinetics of theophylline and its metabolites in rats

Effect of decursin on the pharmacokinetics of theophylline and its metabolites in rats

Quantification of Theophylline in Guinea Pig Plasma by LC-MS/MS Using Hydrophilic Interaction Liquid Chromatography Stationary Phase: Method Development, Validation, and Application in Study

Determination of Caffeine and Its Metabolites in Wastewater Treatment Plants Using Solid-Phase Extraction and Liquid Chromatography–Tandem Mass Spectrometry

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