Development and validation of a reversed-phase liquid chromatographic method for analysis of D4T (Stavudine) in rat plasma

High throughput LC–MS/MS method for simultaneous quantification of lamivudine, stavudine and nevirapine in human plasma

“…Several bioanalytical methods are reported to determine 3TC, d4T and NVP individually in different biological matrices like plasma [16][17][18][19][20][21][22][23][24], tissues and amniotic fluids [16], saliva [17], urine [18,20], cerebrospinal fluids [17,21] and serum [21,25]. Sensitive and selective methods [26][27][28][29][30][31][32][33][34][35][36] based on HPLC-UV and LC-MS/MS methodologies have also been developed for simultaneous analysis of these three drugs in combination with other NRTI, NNRTI or protease inhibitors as they have higher efficacy, acceptable toxicity and broader action range.…”

Oral disintegration tablets of stavudine for HIV management: A new technological approach

“…Plasma concentrations of stavudine were determined following the HPLC procedure as described previously[18]. The mobile phase consisted of methanol:distilled water:acetic acid in 23:77:0.2 (v/v) ratio and was delivered to the system at a flow rate of 1 ml/min.…”

Use of chloramine-T and two dyes in the sensitive determination of stavudine in pharmaceuticals

“…A number of methods based on high performance liquid chromatography (Bezy et al 2005;Verweij-van Wissen et al 2005;Contreras et al 2004), liquid chromatography-tandem mass spectrometry (Compain et al, 2005;Huang et al, 2004;Raices et al, 2003Weisner et al, 2002), micellar electrokinetic chromatography and electrophoresis (Pereira et al, 2005) are known for the quantitative determination of STV in biological matrices such as blood plasma, blood serum and human cells. Several chromatographic techniques including HPLC (Dunge et al, 2005;Djurdjevic et al, 2004;Zhang et al, 2003;Sablon et al, 2004;Pai and Desai.…”