High throughput LC–MS/MS method for simultaneous quantification of lamivudine, stavudine and nevirapine in human plasma

Mistri, Hiren N.; Jangid, Arvind G.; Pudage, Ashutosh; Gomes, Noel A.; Sanyal, Mallika; Shrivastav, Pranav S.

doi:10.1016/j.jchromb.2007.03.047

Cited by 47 publications

(38 citation statements)

References 40 publications

(38 reference statements)

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“…Several LC-MS/MS methods have been reported for the determination of lamivudine, zidovudine, and nevirapine in biological samples either alone or in combination with other drugs (Font et al 1999;Kenney et al 2000;Pereira et al 2000;Chi et al 2003;Mistri et al 2007;Elase et al 2010;Gehrig et al 2007;Murali Krishna et al 2012;Zhou et al 2010). However, these methods had their own problems in respect to the sample preparation, gradient elution, run time, polarity switching, etc.…”

Section: Introductionmentioning

confidence: 99%

A LC–MS/MS method with column coupling technique for simultaneous estimation of lamivudine, zidovudine, and nevirapine in human plasma

Reddy¹,

Thomas²,

Santoshkumar³

et al. 2016

J Anal Sci Technol

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Background: A new LC-MS/MS method for the simultaneous determination of lamivudine, zidovudine, and nevirapine in human plasma is developed using column-coupling technique. Method: Labeled compound of respective analyte was used as an internal standard. After extraction from 100 μL plasma by solid phase extraction method, analytes were separated on a C18 column coupled with a cation exchange column. Total run time was 4.5 min. A tandem mass spectrometric detection was conducted using multiple reaction monitoring under positive ionization mode with an electrospray ionization interface. The method was validated as per the FDA guidelines over the concentration range of 9.47-1466.67 ng/mL for lamivudine, 10. 32-1600.00 ng/mL for zidovudine, and 15.05-2426.67 ng/mL for nevirapine. Results: Precision was in the range 0.86-5.77 (intraday) and 1.92-8.19 (interday) while accuracy was 93.25-104.36 % (intraday) and 96.83-103.28 % (interday). Stabilities of stock in aqueous solutions and in plasma were also determined. Conclusion: The method can be applied to the pharmacokinetic study of a combination treatment.

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Section: Introductionmentioning

confidence: 99%

A LC–MS/MS method with column coupling technique for simultaneous estimation of lamivudine, zidovudine, and nevirapine in human plasma

Reddy¹,

Thomas²,

Santoshkumar³

et al. 2016

J Anal Sci Technol

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“…To be able to evaluate drug exposure a fast, accurate and simple method for determination of isoniazid, rifampin, pyrazinamide and ethambutol in serum is valuable. Like for other antimicrobial drugs, it would be ideal to determine all four compounds in the same sample using a single method of analysis [4,5]. Song et al published a method for the simultaneous determination of all firstline anti-TB drugs using liquid chromatography-tandem mass spectrometry (LC-MS/MS) [6].…”

Section: Introductionmentioning

confidence: 99%

Quantification of isoniazid, pyrazinamide and ethambutol in serum using liquid chromatography-tandem mass spectrometry

Sturkenboom¹,

Lijke²,

Jongedijk³

et al. 2015

J Appl Bioanal

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Clinical studies on tuberculosis have shown a correlation between low drug exposure and treatment failure and acquired drug resistance. Objective was to develop a LC-MS/MS method for the quantification of isoniazid, pyrazinamide and ethambutol. Stable isotope-labelled isoniazid-D4 and ethambutol-D4 were used as internal standards. Protein binding of isoniazid, pyrazinamide and ethambutol was investigated and proved low. Therefore, sample preparation using ultrafiltration could be applied, resulting in linear calibration curves in the range of 0.2-8 mg/L for isoniazid and ethambutol and 2-80 mg/L for pyrazinamide. The method was validated according to the guidelines of the FDA. A fast, simple and reliable LC-MS/MS method has been developed for the simultaneous determination of isoniazid, pyrazinamide and ethambutol in human serum for therapeutic drug monitoring and pharmacokinetic studies.

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“…3 Several analytical methods have been developed for analyzing nevirapine and other antiretroviral drugs including derivative UV spectrophotometry 4 and high performance thin layer chromatography (HPTLC). 5 High performance liquid chromatography (HPLC) has been widely used for determination of antiretrovirals in both biological fluids and pharmaceutical formulations, [6][7][8][9][10][11] hyphenated HPLC-MS [12][13][14][15][16][17] systems have also been introduced for the determination of antiretrovirals in plasma. Many of these methods require the use of expensive and hazardous chemicals which make the process complex, time consuming and costly.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a simple and rapid capillary zone electrophoresis method for determination of nnrti nevirapine in pharmaceutical formulations

Filho

Galdez²,

Silva

et al. 2011

J. Braz. Chem. Soc.

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Um método simples e rápido por eletroforese capilar foi desenvolvido e validado para a quantificação do inibidor não nucleosídeo da transcritase reversa (NNRTI) nevirapina, em medicamentos. A análise foi otimizada utilizando tampão fosfato de sódio 10 mmol L -1 , pH 2,5, voltagem aplicada de +25 kV, injeção hidrodinâmica a 0,5 psi por 5 s e detecção UV em 200 nm. Diazepam (50,0 µg mL -1 ) foi usado como padrão interno. Sob estas condições, nevirapina foi analisada em aproximadamente menos de 2,5 min. A curva analítica apresentou um coeficiente de correlação de 0,9994. Os limites de detecção e quantificação foram 1,4 µg mL -1 e 4,3 µg mL -1 , respectivamente. Precisões intra e inter-dia expressas como desvio padrão relativo foram 1,4% e 1,3%, respectivamente e a recuperação média foi de 100,81%. O fármaco foi submetido a testes de hidrólises (ácida, básica e neutra) e a estresse oxidativo. Não foi observada interferência por parte dos produtos de degradação, nem dos excipients na análise da nevirapina. Este método mostrou ser rápido, simples, preciso, exato e econômico para a determinação de nevirapina em produtos farmacêuticos e é apropriado para o controle de qualidade em análise de rotina uma vez que a eletroforese capilar oferece benefícios em termos de desenvolvimento rápido dos métodos e custos muito reduzidos de operação.A simple and fast capillary zone electrophoresis (CZE) method has been developed and validated for quantification of a non-nucleoside reverse transcriptase inhibitor (NNRTI) nevirapine, in pharmaceuticals. The analysis was optimized using 10 mmol L -1 sodium phosphate buffer pH 2.5, +25 kV applied voltage, hydrodynamic injection 0.5 psi for 5 s and direct UV detection at 200 nm. Diazepam (50.0 µg mL -1 ) was used as internal standard. Under these conditions, nevirapine was analyzed in approximately less than 2.5 min. The analytical curve presented a coefficient of correlation of 0.9994. Limits of detection and quantification were 1.4 µg mL -1 and 4.3 µg mL -1 , respectively. Intra-and inter-day precision expressed as relative standard deviations were 1.4% and 1.3%, respectively and the mean recovery was 100.81%. The active pharmaceutical ingredient was subjected to hydrolysis (acid, basic and neutral) and oxidative stress conditions. No interference of degradation products and tablet excipients were observed. This method showed to be rapid, simple, precise, accurate and economical for determination of nevirapine in pharmaceuticals and it is suitable for routine quality control analysis since CE offers benefits in terms of quicker method development and significantly reduced operating costs.

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High throughput LC–MS/MS method for simultaneous quantification of lamivudine, stavudine and nevirapine in human plasma

Cited by 47 publications

References 40 publications

A LC–MS/MS method with column coupling technique for simultaneous estimation of lamivudine, zidovudine, and nevirapine in human plasma

A LC–MS/MS method with column coupling technique for simultaneous estimation of lamivudine, zidovudine, and nevirapine in human plasma

Quantification of isoniazid, pyrazinamide and ethambutol in serum using liquid chromatography-tandem mass spectrometry

Development and validation of a simple and rapid capillary zone electrophoresis method for determination of nnrti nevirapine in pharmaceutical formulations

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