Development and Validation of a Bioanalytical Method for Direct Extraction of Diclofenac Potassium from Spiked Plasma

Sarfraz, Attia; Sarfraz, Muhammad; Ahmad, Mahmood

doi:10.4314/tjpr.v10i5.16

Cited by 5 publications

(4 citation statements)

References 11 publications

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“…Validation of the developed method was performed under the International Conference on Harmonization guidelines and Guidance for Industry: Bioanalytical Method Validation [ 21 , 22 ]. The developed method was further validated against spiked plasma samples under strict compliance as we did previously [ 23 , 24 ]. Before lysis the liposome to determine OA concentration, the free OA was separated from the OA encapsulated liposomes.…”

Section: Methodsmentioning

confidence: 99%

Development of Dual Drug Loaded Nanosized Liposomal Formulation by A Reengineered Ethanolic Injection Method and Its Pre-Clinical Pharmacokinetic Studies

et al. 2018

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Oleanolic acid (OA), which is a natural pentacyclic terpenoid, has been identified for hepato-protective, nephron-protective and cardio-tonic properties. In contrast, doxorubicin (DOX) is a famous anti-cancer drug but its efficacy is a question mark because of its known cardio-toxicity. We developed a combined nanoliposomal formulation of DOX with OA, as adjuvant, to overwhelm toxic effects of DOX without compromising anticancer activity. The entrapment efficiency and the particle size were brought in limit by the reengineered ethanolic injection method (REIM), without further extrusion. The developed formulations were stable over the study period of two months. A modified HPLC method was employed for the analysis of OA (drug retention time, Tr = 12 ± 1 min). The recovery of OA against spiked plasma samples was more than 90%. MTT assay showed anti-apoptotic synergism against HepG2 cells at non-fixed ratio (combination index, CI < 1). A sustained in vivo drug release of experimental drugs was depicted over 24 h. Histopathological examination and laboratory findings indicated no visible sign of toxicity in the treated mice group against combined delivery. Hence, this combined nanoliposomal formulation was tagged as a safer therapy for the DOX based cancer treatments.

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Section: Methodsmentioning

confidence: 99%

Development of Dual Drug Loaded Nanosized Liposomal Formulation by A Reengineered Ethanolic Injection Method and Its Pre-Clinical Pharmacokinetic Studies

et al. 2018

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“…Reported dissociation constant (pKa) is found to be 4.0 ± 0.2 at 25°C in water while the partition coefficient in n-octanol at pH 7.4 is 13.4 and at pH 5.2 is 1545. [44][45]…”

Section: Introductionmentioning

confidence: 99%

Diclofenac Potassium

Ali

Zafar²,

Baloch³

et al. 2016

TPMJ

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“…Reported dissociation constant (pKa) is found to be 4.0 ± 0.2 at 25°C in water while the partition coefficient in n-octanol at pH 7.4 is 13.4 and at pH 5.2 is 1545. [44][45]…”

Section: Introductionmentioning

confidence: 99%