Development and validation of a novel RP-HPLC method for simultaneous determination of paracetamol, phenylephrine hydrochloride, caffeine, cetirizine and nimesulide in tablet formulation

Dewani, Anil P.; Dabhade, S.M.; Bakal, Ravindra L.; Gadewar, CK; Chandewar, A. V.; Patra, Sradhanjali

doi:10.1016/j.arabjc.2013.09.040

Cited by 54 publications

(23 citation statements)

References 18 publications

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“…Phenylephrine hydrochloride (PHE), (R)‐2‐methylamino‐1‐3(‐hydroxyphenyl)ethanol hydrochloride (Figure c) is a widely applied effective sympathomimetic agent that has vasoconstrictor effects and is used as a nasal decongestant to relieve severe cold symptoms . Various analytical methods have been reported for the quantitation of PHE in different pharmaceutical forms using spectrophotometric methods, spectrofluorometric methods, HPLC, electrochemical analysis and CE …”

Section: Introductionmentioning

confidence: 99%

Synchronous spectrofluorometric methods for simultaneous determination of diphenhydramine and ibuprofen or phenylephrine in combined pharmaceutical preparations

2020

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Simple and rapid synchronous fluorometric methods were adopted and validated for the simultaneous analysis of a binary mixture of diphenhydramine (DIP) and ibuprofen (IBU) (Mix I) or DIP and phenylephrine (PHE) (Mix II) in their co-formulated pharmaceuticals without prior separation. Analysis of Mix I is based on the measurement of the peak amplitudes (D 1 ) of synchronous fluorescence intensities at 265.1 nm for DIP and 260 nm for IBU. The relationship between the concentration and the amplitude of the first-derivative synchronous fluorescence spectra showed good linearity over the concentration ranges 0.50-10.00 μg ml −1 and 0.50-7.90 μg ml −1 for DIP and IBU, respectively. Analysis of Mix II was based on measurement of the peak amplitude (D 1 ) synchronous fluorescence intensities at 230 nm for DIP and at 253.9 nm for PHE. Moreover, for Mix II, the peak amplitude (D 2 ) synchronous fluorescence intensities were measured at 227.9 nm for DIP and at 264.9 nm for PHE. Calibration plots were rectilinear over the concentration range 0.30-3.50 μg ml −1 and 0.03-0.75 μg ml −1 for DIP and PHE, respectively. The proposed methods were successfully applied to determine the studied compounds in pure form and in pharmaceutical preparations. K E Y W O R D Sibuprofen, diphenhydramine, phenylephrine, synchronous fluorescence, pharmaceutical preparations

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Section: Introductionmentioning

confidence: 99%

Synchronous spectrofluorometric methods for simultaneous determination of diphenhydramine and ibuprofen or phenylephrine in combined pharmaceutical preparations

2020

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“…Ultra-performance liquid chromatography (UPLC) is a new category of separation technique based upon well-established principles of liquid chromatograph 4,5 . UPLC provides the benefit of small injection volumes, shortened run times (<5 min), and reduced solvent usage, making it the more economical method for quantitation 6 .…”

Section: Introductionmentioning

confidence: 99%

Development and validation of uplc method for the determination of olopatadine hydrochloride in polymeric nanoparticles

Güven¹,

Berkman²,

Yazan³

2019

actapharm

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A new, simple, rapid, precise, accurate and specific stability indicating reverse phase UPLC method developed for the determination of encapsulated olopatadine hydrochloride (OLO) in the polymeric nanoparticle formulations. Studies were carried out on a 2.1x50 mm, 1.8 µm Zorbax Eclipse Plus C 18 column with an optimized mobile phase of methanol, water and sodium acetate buffer solution (40:50:10, v/v/v) at a flow rate of 0.5 mL/min. OLO was detected and quantitated using a photodiode array detector at a wavelength of 246 nm and the column temperature was adjusted to 40 °C. The proposed method validation was carried out for specificity, linearity, accuracy, precision, limit of detection, limit of quantitation and robustness according to the ICH harmonised tripartite guideline "validation of analytical procedures Q2(R1)". Analytical curve was linear over the concentration range of 5-50 µg/mL. All the validation parameters were within the acceptance range. LOD and LOQ for OLO were 0.7652 and 2.3188 µg/mL, respectively. The developed method fulfilled the requirements for reliability and feasibility for the quantitative analysis of OLO in polymeric nanoparticles.

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“…Various methods reported in literature for analysis of phenylephrine hydrochloride. Examples of these methods are conductometric titration [6], voltammetry [7][8][9], thin-layer chromatography [10], high-performance liquid chromatography (HPLC) [11][12][13][14], flow injection [15][16][17], and fluorescence [18]. Among the different techniques, the most popular and simple method for rapid and trace analysis of drugs is spectrophotometry [19][20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Determination of Phenylephrine Hydrochloride in Pharmaceutical Preparations Using Spectrophotometric Method

Al-Uzri

2019

Asian J Pharm Clin Res

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Objective: A simple and sensitive spectrophotometric method has been presented for the determination of phenylephrine hydrochloride by coupling reaction with diazotized sulfacetamide sodium. Methods: The method is based on the diazotization reaction of sulfacetamide sodium with sodium nitrite in the presence of hydrochloric acid to form diazonium salt, which is coupled with the drug in alkaline medium to form azo dye, showing absorption maxima at 425 nm. Results: Calibration plot was linear over the concentration range of 2–24 μg/mL and detection limit of 0.278 μg/mL with a correlation coefficient of 0.9929. All different chemical and physical experimental parameters affecting on the development and stability of the colored product were carefully studied. Conclusions: The proposed method was successfully applied to the determination of phenylephrine in nasal drops with good precision and sensitivity.

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Development and validation of a novel RP-HPLC method for simultaneous determination of paracetamol, phenylephrine hydrochloride, caffeine, cetirizine and nimesulide in tablet formulation

Cited by 54 publications

References 18 publications

Synchronous spectrofluorometric methods for simultaneous determination of diphenhydramine and ibuprofen or phenylephrine in combined pharmaceutical preparations

Synchronous spectrofluorometric methods for simultaneous determination of diphenhydramine and ibuprofen or phenylephrine in combined pharmaceutical preparations

Development and validation of uplc method for the determination of olopatadine hydrochloride in polymeric nanoparticles

Determination of Phenylephrine Hydrochloride in Pharmaceutical Preparations Using Spectrophotometric Method

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