Development and validation of uplc method for the determination of olopatadine hydrochloride in polymeric nanoparticles

Güven, Umay Merve; Berkman, Murat Sami; Yazan, Yasemin

doi:10.23893/1307-2080.aps.05701

Cited by 5 publications

(4 citation statements)

References 14 publications

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“…Since the RSD% values for both intermediate precision and repeatability were <2%, the method developed for theophylline was found to be precise according to the suggestions in ICH Q2(R1) guidelines [22].…”

Section: Results Of Precision and Repeatability Tests On Different Concentrations Are Given In Tablementioning

confidence: 95%

Preparation, characterization and in-vitro evaluation of theophylline loaded microemulsion formulations

Çevikelli¹,

Onan²,

Güven³

et al. 2020

jpt

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Section: Results Of Precision and Repeatability Tests On Different Concentrations Are Given In Tablementioning

confidence: 95%

Preparation, characterization and in-vitro evaluation of theophylline loaded microemulsion formulations

Çevikelli¹,

Onan²,

Güven³

et al. 2020

jpt

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“… 117 Among liquid chromatographic techniques, UPLC has several advantages as it needs the sample in small volumes, shorter run times with decreased use of solvents and therefore is an economical method for rapid quantification of biomedical agents. 118 With this rationale, the data retrieved by UPLC was used in determining the quantitative load of rutin on rCNPs. In this regard, the retention time of standard rutin was 18.735 min; comparatively, the retention time for 0.22 micron filtered samples was 19.077 min ( Supplementary Figure 1A and B ).…”

Section: Resultsmentioning

confidence: 99%

ULK1 Mediated Autophagy-Promoting Effects of Rutin-Loaded Chitosan Nanoparticles Contribute to the Activation of NF-κB Signaling Besides Inhibiting EMT in Hep3B Hepatoma Cells

Wu,

Wang,

Yin

et al. 2024

IJN

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Background Liver cancer remains to be one of the leading causes of cancer worldwide. The treatment options face several challenges and nanomaterials have proven to improve the bioavailability of several drug candidates and their applications in nanomedicine. Specifically, chitosan nanoparticles (CNPs) are extremely biodegradable, pose enhanced biocompatibility and are considered safe for use in medicine. Methods CNPs were synthesized by ionic gelation, loaded with rutin (rCNPs) and characterized by ultraviolet–visible spectroscopy (UV-Vis), Fourier-transform infrared spectroscopy (FTIR), dynamic light scattering (DLS) and transmission electron microscopy (TEM). The rCNPs were tested for their cytotoxic effects on human hepatoma Hep3B cells, and experiments were conducted to determine the mechanism of such effects. Further, the biocompatibility of the rCNPs was tested on L929 fibroblasts, and their hemocompatibility was determined. Results Initially, UV–vis and FTIR analyses indicated the possible loading of rutin on rCNPs. Further, the rutin load was quantitatively measured using Ultra-Performance Liquid Chromatography (UPLC) and the concentration was 88 µg/mL for 0.22 micron filtered rCNPs. The drug loading capacity (LC%) of the rCNPs was observed to be 13.29 ± 0.68%, and encapsulation efficiency (EE%) was 19.55 ± 1.01%. The drug release was pH-responsive as 88.58% of the drug was released after 24 hrs at the lysosomal pH 5.5, whereas 91.44% of the drug was released at physiological pH 7.4 after 102 hrs. The cytotoxic effects were prominent in 0.22 micron filtered samples of 5 mg/mL rutin precursor. The particle size for the rCNPs at this concentration was 144.1 nm and the polydispersity index (PDI) was 0.244, which is deemed to be ideal for tumor targeting. A zeta potential (ζ-potential) value of 16.4 mV indicated rCNPs with good stability. The IC 50 value for the cytotoxic effects of rCNPs on human hepatoma Hep3B cells was 9.7 ± 0.19 μg/mL of rutin load. In addition, the increased production of reactive oxygen species (ROS) and changes in mitochondrial membrane potential (MMP) were observed. Gene expression studies indicated that the mechanism for cytotoxic effects of rCNPs on Hep3B cells was due to the activation of Unc-51-like autophagy-activating kinase (ULK1) mediated autophagy and nuclear factor kappa B (NF-κB) signaling besides inhibiting the epithelial–mesenchymal Transition (EMT). In addition, the rCNPs were less toxic on NCTC clone 929 (L929) fibroblasts in comparison to the Hep3B cells and possessed excellent hemocompatibility (less than 2% of hemolysis). Conclusion The synthesized rCNPs were pH-responsive and possessed the physicochemical properties suitable for tumor targeting. The particles were effectively cytotoxic on Hep3B cells in comparison to normal cells and possessed excellent hemocompatibility. The very low hemolytic profile of rCNPs indicates that the drug c...

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“…It is reported that polymeric NPs fabricated using spray drying module to control particle size and distribution. The NPs obtained by spray-dryer showed good performance in terms of a high yield and encapsulation efficiency [16,17]. Polymer types, polymer concentration and amount of drug were optimized.…”

Section: Preparation Of Polymeric Nanoparticlesmentioning

confidence: 99%

Evaluation of carvedilol-loaded Eudragit® nanoparticles

Güven¹,

Öztürk²,

Yenilmez³

2020

jrp

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The objective of present study was to prepare positively charged carvedilol-loaded nanoparticles providing a controlled release formulation by spray dryer technique and was to examine the effect of different derivative of Eudragit ® polymers on entrapment efficiency (EE%), dissolution profile and release kinetics. Two nonbiodegradable positively charged polymers, Eudragit ® RS100 and RL100 were used alone or in combination. The prepared formulations were evaluated for their particle size, polydispersity index, zeta potential, in vitro dissolution and in vitro release kinetics study. Particle sizes of placebo nanoparticles and carvedilol loaded nanoparticles were 418.2±18.5/ 402.3±22.0/ 416.3±12.5 and 535.5±13.8/ 529.4±10.2/ 530.4±10.4 nm, respectively with PDI values of approximately 0.4-0.6 for each. Carvedilol loading was resulted in positive electrical charge on nanoparticles. The drug encapsulation efficiency was 68.45±4.67/ 63.58±2.30/ 65.67±4.21. In vitro cumulative release from the nanoparticles was 80-90 % at 48 hour. Morphology and solid state analyzes were performed also in nanoparticles. The results of this research indicate that spray dryer technique is suitable for carvedilol loaded Eudragit ® nanoparticles and no burst effect but a prolonged drug release was observed from formulations.

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Development and validation of uplc method for the determination of olopatadine hydrochloride in polymeric nanoparticles

Cited by 5 publications

References 14 publications

Preparation, characterization and in-vitro evaluation of theophylline loaded microemulsion formulations

Preparation, characterization and in-vitro evaluation of theophylline loaded microemulsion formulations

ULK1 Mediated Autophagy-Promoting Effects of Rutin-Loaded Chitosan Nanoparticles Contribute to the Activation of NF-κB Signaling Besides Inhibiting EMT in Hep3B Hepatoma Cells

Evaluation of carvedilol-loaded Eudragit® nanoparticles

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