Development and Validation of a UV-Spectrophotometric Method for Determination of Flucloxacillin Sodium in Capsules

Abstract

Flucloxacillin (FLU) is a semi-synthetic penicillin active against many gram-positive bacteria such as streptococci and penicilinase-producing staphylococci, including methicillin-susceptible S. aureus. A simple, rapid, reproducible and cost-effective UV-spectrophotometric analytical method was validated to assay flucloxacillin sodium (FLU) in capsules. Measurements were taken at 274 nm using ultra-purified water as the solvent. This new method was validated in accordance with ICH requirements, which include linearity, precision, accuracy, specificity, robustness, detection and quantitation limits. The method demonstrated good linearity over the range of 50.0 to 100.0 mg L -1 FLU with a correlation coefficient (r) equal to 0.9998. The accuracy of the method was 100.09%. The precision demonstrated a relative standard deviation of less than 2.5%. The excipient in the capsules did not interfere with the assay. The results were satisfactory when compared with the literature. The proposed method might be applied in routine quality control in the pharmaceutical industries since it is precise, accurate, simple, economic and produces very low amounts of solvents and residues.

Besifloxacin: A Critical Review of Its Characteristics, Properties, and Analytical Methods

“…[61] Spectrophotometry in the UV region is an instrumental method widely used for the quantification of drugs, since it is considered simple, fast, economical, and reproducible. [62][63][64][65] However, it is a non-selective method and is not suitable for the detection of degradation products and impurities. [66] Spectrophotometric methods are also found in literature for other fluoroquinolones, such as ciprofloxacin, [67] norfloxacin, [68,69] orbifloxacin, [70] lomefloxacin, [71] gatifloxacin, [72] and sparfloxacin.…”

A green approach for the quantification of daptomycin in pharmaceutical formulation by UV spectrophotometry

“…In addition, they can be developed without the use of organic solvents, which makes this technique more environmentalfriendly (Moreno, Salgado, 2009; Bonfílio, Araújo, Salgado, 2011;Cazedey, Salgado, 2012;Fiorentino, Salgado, 2012;Silva, Almeida, Salgado, 2012;Pedroso, Salgado, 2013;Cazedey, Salgado, 2014;Chierentin, Salgado, 2014;Corrêa, Serra, Salgado, 2014). To our knowledge, there are no studies in the literature that used spectrophotometry to analyze daptomycin.…”

A CdTe–MPA quantum dot fluorescence enhancement flow method for chlorhexidine determination

“…1), is an antiseptic drug with a biguanidic structure active against Gram-negative and Gram-positive bacteria. Several methods, recently reviewed, 13 have been proposed for chlorhexidine determination in pharmaceuticals and biological samples. These methods include chromatography, [14][15][16][17] uorimetry, 18 UV/Vis spectrophotometry 19 and capillary electrophoresis.…”