Development and validation of a fast SFC method for the analysis of flavonoids in plant extracts

Huang, Youliang; Feng, Yi; Tang, Guangyun; Li, Minyi; Zhang, Tingting; Fillet, Marianne; Crommen, Jacques; Jiang, Zhengjin

doi:10.1016/j.jpba.2017.03.012

Cited by 47 publications

(21 citation statements)

References 42 publications

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“…Almost all stationary phases used in HPLC are also feasible for SFC and diverse detectors such as diode array detector (DAD), evaporative light scattering detector (ELSD), MS, DC or charged aerosol detector (CAD) can be fitted to the instrument [ 268 , 269 ]. For plant extracts SFC has already been used for the isolation of nonpolar compounds such as terpenes [ 270 ] or flavonoids [ 271 ] but it also has proven to be a useful tool for the isolation of more polar natural products such as carsonic acid from rosemary extracts [ 272 ] and valerenic acid from Valeriana officinalis [ 273 ]. Notwithstanding, to our knowledge SFC has never been used for the discovery of new antibiotics from actinomycetes.…”

Section: Metabolomics For the Discovery Of New Antibiotics Producementioning

confidence: 99%

Concepts and Methods to Access Novel Antibiotics from Actinomycetes

et al. 2018

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Actinomycetes have been proven to be an excellent source of secondary metabolites for more than half a century. Exhibiting various bioactivities, they provide valuable approved drugs in clinical use. Most microorganisms are still untapped in terms of their capacity to produce secondary metabolites, since only a small fraction can be cultured in the laboratory. Thus, improving cultivation techniques to extend the range of secondary metabolite producers accessible under laboratory conditions is an important first step in prospecting underexplored sources for the isolation of novel antibiotics. Currently uncultured actinobacteria can be made available by bioprospecting extreme or simply habitats other than soil. Furthermore, bioinformatic analysis of genomes reveals most producers to harbour many more biosynthetic gene clusters than compounds identified from any single strain, which translates into a silent biosynthetic potential of the microbial world for the production of yet unknown natural products. This review covers discovery strategies and innovative methods recently employed to access the untapped reservoir of natural products. The focus is the order of actinomycetes although most approaches are similarly applicable to other microbes. Advanced cultivation methods, genomics- and metagenomics-based approaches, as well as modern metabolomics-inspired methods are highlighted to emphasise the interplay of different disciplines to improve access to novel natural products.

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Section: Metabolomics For the Discovery Of New Antibiotics Producementioning

confidence: 99%

Concepts and Methods to Access Novel Antibiotics from Actinomycetes

et al. 2018

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“…Indeed, there have already been developments and successful implementations in the past, however, now the constantly growing use of high resolutions MS instruments (HRMS), hyphenated not only to LC but also to SFC, has pushed the latter even further in this area. Besides the analysis of lipophilic compounds including lipids in plants [56][57][58][59], there is an interesting and growing trend, namely the analysis of compounds with increasing polarity, such as monosaccharides [60], saponins [61] and flavonoids [62]. Other natural compounds are also being analyzed under SFC-MS, such as plant metabolites with interesting potential as drugs (Fig.…”

Section: Applications Of Sfc-msmentioning

confidence: 99%

Supercritical fluid chromatography – Mass spectrometry: Recent evolution and current trends

Losacco

Veuthey

Guillarme

2019

TrAC Trends in Analytical Chemistry

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• A detailed summary on SFC-MS interfaces is given, with emphasis on current issues and potential solutions. • Differences between LC-MS and SFC-MS in terms of matrix effects generated are highlighted. • Sensitivity under SFC-MS has been demonstrated to be comparable to what it can be reached in LC-MS conditions. • Applications for SFC-MS are shifting towards the analysis of compounds with increasing polarity and analytes available in complex matrices.

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“…For this task, phosphoric acid (0.15% v/v) was added to MeOH and the column screening with the Fluoro Phenyl, BEH 2-EP, DIOL columns was repeated. Phosphoric acid was selected as acidic additive, as in previous studies, dealing with the SFC analysis of similar compounds, it was found to be most appropriate, explained by the minor baseline drift compared to other acidic additives at one side and its lower pK a at the other side [33,34]. However, only on the Acquity UPC 2 Torus DIOL column, a hybrid silica stationary phase with high-density diol ligands, a significant improvement could be observed, resulting finally in good peak shape and satisfying resolution for all compounds.…”

Section: F I G U R E 1 Chemical Structures Of the Major Compounds Inmentioning

confidence: 99%

Towards eco‐friendly secondary plant metabolite quantitation: Ultra high performance supercritical fluid chromatography applied to common vervain (Verbena officinalis L.)

Gibitz-Eisath

Eichberger

Gruber

et al. 2019

J of Separation Science

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This report presents the first ultra high performance supercritical fluid chromatography diode array detector based assay for simultaneous determination of iridoid glucosides, flavonoid glucuronides, and phenylpropanoid glycosides in Verbena officinalis (Verbenaceae) extracts. Separation of the key metabolites was achieved in less than 7 min on an Acquity UPC2 Torus Diol column using a mobile phase gradient comprising subcritical carbon dioxide and methanol with 0.15% phosphoric acid. Method validation for seven selected marker compounds (hastatoside, verbenalin, apigenin‐7‐O‐glucuronide, luteolin‐7‐O‐glucuronide, apigenin‐7‐O‐diglucuronide, verbascoside, and luteolin‐7‐O‐diglucuronide) confirmed the assay to be sensitive, linear, precise, and accurate. Head‐to‐head comparison to an ultra high performance liquid chromatography comparator assay did prove the high orthogonality of the methods. Quantitative result equivalence was evaluated by Passing‐Bablok‐correlation and Bland‐Altman‐plot analysis. This cross‐validation revealed, that one of the investigated marker compound peaks was contaminated in the ultra high performance liquid chromatography assay by a structurally related congener. Taken together, it was proven that the ultra high performance supercritical fluid chromatography instrument setup with its orthogonal selectivity is a true alternative to conventional reversed phase liquid chromatography in quantitative secondary metabolite analysis. For regulatory purposes, assay cross‐validation with highly orthogonal methods seems a viable approach to avoid analyte overestimation due to coeluting, analytically indistinguishable contaminants.

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Development and validation of a fast SFC method for the analysis of flavonoids in plant extracts

Cited by 47 publications

References 42 publications

Concepts and Methods to Access Novel Antibiotics from Actinomycetes

Concepts and Methods to Access Novel Antibiotics from Actinomycetes

Supercritical fluid chromatography – Mass spectrometry: Recent evolution and current trends

Towards eco‐friendly secondary plant metabolite quantitation: Ultra high performance supercritical fluid chromatography applied to common vervain (Verbena officinalis L.)

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