Supercritical fluid chromatography – Mass spectrometry: Recent evolution and current trends

Losacco, Gioacchino Luca; Veuthey, Jean‐Luc; Guillarme, Davy

doi:10.1016/j.trac.2019.07.005

Cited by 63 publications

(25 citation statements)

References 84 publications

(141 reference statements)

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“…In fact, more selectivity could be gained thanks to the MS detector, which would allow having a specific response depending on the differences concerning mass/charge ratios of the analytes. Several studies have demonstrated that UHPSFC can be successfully coupled to MS detectors, and this chromatographic method can be easily transferred to MS detection by integrating it with an appropriate make‐up solution to promote analyte ionization (for instance, in ESI‐MS, MeOH/water 98/2% v/v with 0.1% of ammonium formate) 25 . However, some considerations must be made in this context.…”

Section: Resultsmentioning

confidence: 99%

The analysis of cannabinoids in cannabis samples by supercritical fluid chromatography and ultra‐high‐performance liquid chromatography: A comparison study

Deidda

Schelling

Roussel³

et al. 2020

Analytical Science Advances

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The aim of this work was to develop a supercritical fluid chromatographic method to study the applicability of this emerging technique to cannabinoid analysis and showcase its advantages. During method development, the authors focused on nine phyto‐cannabinoids to assess the selectivity needed to potentially perform the quantitation of each cannabinoid. After method development, robustness studies were carried out on this method to gain more information about its qualitative behavior (in terms of critical resolutions) when varying some crucial parameters (concentration of additive, column temperature, starting gradient conditions and column batch). Once the robustness was evaluated and the parameters most affecting the selected responses were individuated, the SFC method was applied for a simulated routine use to generate quantitative results concerning the concentrations of the main cannabinoids in real cannabis samples. The samples were also analyzed by means of an ultra‐high‐performance liquid chromatographic method currently used in the laboratory for the same objective. Finally, the results obtained with both analytical methods were compared to evaluate their accordance. The Bland–Altman method was applied as a statistical strategy to evaluate the degree of accordance between the results generated and display the data in a difference plot. The ultra‐high performance supercritical fluid chromatography quantitative results were in accordance with the ultra‐high performance liquid chromatography results, demonstrating the applicability of this technique for cannabinoid analysis.

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Section: Resultsmentioning

confidence: 99%

The analysis of cannabinoids in cannabis samples by supercritical fluid chromatography and ultra‐high‐performance liquid chromatography: A comparison study

Deidda

Schelling

Roussel³

et al. 2020

Analytical Science Advances

View full text Add to dashboard Cite

show abstract

“…24) Moreover, a packed column, filled with bare silica, which was developed as a stationary phase for HPLC, was shown to be applicable to SFC. 25) Upon supercritical carbon dioxide silica gel column chromatography, the chromatographic behaviors of hydrophobic compounds were similar to those on normal-phase HPLC. In addition, SFC gave a better separation of chiral compounds when compared with HPLC.…”

Section: Analytical Chemistry In Biology and Medicinementioning

confidence: 88%

Emerging Separation Techniques in Supercritical Fluid Chromatography

Yamamoto

Machida

Kotani

et al. 2021

CHEMICAL & PHARMACEUTICAL BULLETIN

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Supercritical fluid chromatography (SFC) has unique separative characteristics distinguished from those of HPLC and gas chromatography. At present, SFC is widely used and there are many applications in various biological, medical, and pharmaceutical fields. In this review, we focus on recently developed novel techniques related to SFC separation including: new column stationary phases, microfluidics, twodimensional separation, and gas-liquid separation. In addition, we discuss the application of SFC using a water-containing modifier to biological molecules such as amino acids, peptides, and small proteins that had been challenging analytes.

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“…Supercritical fluid chromatography (SFC) is an alternative technique for separation of chiral compounds and provides several advantages over HPLC. [12][13][14][15] The mobile phase contains a large fraction of carbon dioxide, which has lower viscosity and higher diffusivity than liquid chromatographic solvents. As a consequence, high flow rates can be used without loss of resolution or efficiency and shorter equilibration times are achieved.…”

Section: Several High Performance Liquid Chromatography (Hplc) Methodsmentioning

confidence: 99%

Enantiomeric separation and quantification of R/S‐amphetamine in serum using semi‐automated liquid‐liquid extraction and ultra‐high performance supercritical fluid chromatography‐tandem mass spectrometry

Havnen

Hansen

Spigset

et al. 2020

Drug Testing and Analysis

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The amphetamine molecule contains a chiral center and its enantiomers exhibit differences in pharmacological effects, with the S‐enantiomer mediating most of the central nervous system stimulating activity. The majority of prescribed amphetamine consists of the pure S‐enantiomer, but therapeutic formulations containing the R‐enantiomer in various proportions are also available. Illegal amphetamine remains available mainly as a racemic mixture of the R‐ and S‐enantiomers. To distinguish between legal and illegal consumption of amphetamine a method for enantiomeric separation and quantification of R/S‐amphetamine in serum was developed and validated using ultra‐high performance supercritical fluid chromatography‐tandem mass spectrometry (UHPSFC‐MS/MS). Sample preparation prior to UHPSFC‐MS/MS analysis was performed by a semi‐automated liquid–liquid extraction method. The UHPSFC‐MS/MS method used a Chiralpak AD‐3 column with a mobile phase consisting of CO2 and 0.1% ammonium hydroxide in 2‐propanol/methanol (50/50, v/v). The injection volume was 2 μL and run time was 4 minutes. MS/MS detection was performed with positive electrospray ionization and two multiple reaction monitoring transitions (m/z 136.1 > 119.0 and m/z 136.1 > 91.0). The calibration range was 12.5–1,000 nM for each analyte. The between‐assay relative standard deviations were in the range of 1.3–3.0%. Recovery was 73% and matrix effects ranged from 95 to 100% when corrected with internal standard. After development and validation, the method has been successfully implemented in our laboratory for both separation and quantification of R/S‐amphetamine and has proved to be a reliable and useful tool for distinguishing intake of R‐ and S‐amphetamine in authentic patient samples.

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Supercritical fluid chromatography – Mass spectrometry: Recent evolution and current trends

Cited by 63 publications

References 84 publications

The analysis of cannabinoids in cannabis samples by supercritical fluid chromatography and ultra‐high‐performance liquid chromatography: A comparison study

The analysis of cannabinoids in cannabis samples by supercritical fluid chromatography and ultra‐high‐performance liquid chromatography: A comparison study

Emerging Separation Techniques in Supercritical Fluid Chromatography

Enantiomeric separation and quantification of R/S‐amphetamine in serum using semi‐automated liquid‐liquid extraction and ultra‐high performance supercritical fluid chromatography‐tandem mass spectrometry

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